Teste de tetrazólio para avaliação da qualidade de sementes de Tabebuia roseoalba (Ridl.) Sandwith - bignoniaceae, submetidas ao armazenamento

Abbade, Leticia Caravita; Takaki, Massanori

doi:10.1590/s0100-67622014000200003

Cited by 36 publications

(23 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(LAMARCA;LEDUC;BARBEDO, 2009), Tabebuia roseo-alba (Ridley) Sandwith. (ABBADE;TAKAKI, 2014) e Simira gardneriana M.R. Barbosa & Peixoto (OLIVEIRA et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Viability and vigour in seeds of Poincianella pyramidalis (Tul.) L. P. Queiroz by tetrazolium test

Sousa

Bruno

Silva

et al. 2017

Revista Ciência Agronômica

View full text Add to dashboard Cite

-The Caatinga has a wide diversity of species with potential for exploitation, among which is the catingueira (Poincianella pyramidalis (Tul.) L. P. Queiroz), which is notable for its importance in the areas of timber and forestry. The use of rapid tests in quality control programs for seeds is an essential tool for evaluating physiological quality. The aim of this study was therefore to develop procedures for a tetrazolium test to evaluate viability and vigour in seeds of P. pyramidalis. Two procedures for preconditioning the seeds were tested: between moist paper towels and directly in water. In preparing the seeds, both manual scarification and cutting the seed at the opposite end to the hilum were tested. For staining, two concentrations of tetrazolium solution were used (0.075% and 0.15%) and three staining periods (30, 60 and 90 minutes), with the seeds being sorted into classes of viability and vigour. The experimental design was completely randomised in a 3 x 3 x 2 factorial scheme (harvest year x staining time x tetrazolium concentration), with four replications of 25 seeds. The tetrazolium test was efficient in assessing viability and vigour in seeds of P. pyramidalis. After cutting the opposite end to the hilum, the seeds were preconditioned directly in water for 24 hours at 25 °C, when the integument could be removed. Staining was achieved by immersing the seeds in a 0.075% tetrazolium solution for 90 minutes at 41 °C.

show abstract

“…(LAMARCA;LEDUC;BARBEDO, 2009), Tabebuia roseo-alba (Ridley) Sandwith. (ABBADE;TAKAKI, 2014) e Simira gardneriana M.R. Barbosa & Peixoto (OLIVEIRA et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Viability and vigour in seeds of Poincianella pyramidalis (Tul.) L. P. Queiroz by tetrazolium test

Sousa

Bruno

Silva

et al. 2017

Revista Ciência Agronômica

View full text Add to dashboard Cite

show abstract

“…], para o qual é recomendada a concentração de 0,1%, a 30°C, por 24 horas (Pinho et al, 2011), e ipê-branco [Tabebuia roseoalba (Ridl.) Sandwith], para o qual é indicada a concentração de 0,5%, a 36°C, durante 24 horas (Abbade & Takaki, 2014). No caso da araucária, Oliveira et al (2014) obtiveram melhores resultados com as concentrações de 0,1 ou 0,5% por 1 hora, a 25°C, porém incluíram um período de pré-umedecimento das sementes por 18 horas antes da extração dos embriões, o que acarretou expressivo aumento do tempo de análise.…”

Section: Resultsunclassified

Critérios para condução do teste de tetrazólio em sementes de araucária

Silva

Nogueira

Vieira

et al. 2016

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi desenvolver critérios para condução do teste de tetrazólio em sementes de araucária (Araucaria angustifolia), com maior rapidez de execução e caracterização detalhada dos grupos de viabilidade. Inicialmente, foram conduzidos testes de germinação e de tetrazólio, de acordo as Regras para Análise de Sementes para Araucaria spp. Em seguida, avaliaram-se procedimentos mais práticos para condução do teste de tetrazólio, sem o pré-umedecimento das sementes e com a utilização de embriões excisados imersos diretamente na solução de tetrazólio; foram testadas três concentrações da solução (0,075, 0,2 e 0,5%), três períodos de coloração (1, 2 e 4 horas) e duas temperaturas de coloração (30 e 40°C). As médias obtidas por cada teste realizado foram comparadas pelo teste de Scott-Knott, a 1% de probabilidade. O teste de tetrazólio pode ser empregado com eficiência e maior rapidez por meio da excisão dos embriões de araucária e da imersão direta na solução de tetrazólio, ao se utilizar as seguintes combinações de concentração/período/temperatura: 0,5%/2 horas/30°C, 0,075%/4 horas/40°C ou 0,5%/2 horas/40°C, as quais permitem a classificação das sementes em dois grupos distintos de viabilidade (viáveis e inviáveis).Termos para indexação: Araucaria angustifolia, embrião excisado, grupo de viabilidade. Criteria for conduction of tetrazolium test on araucaria seedsAbstract -The objective of this work was to determine criteria for the conduction of tetrazolium test on araucaria (Araucaria angustifolia) seeds, with greater execution speed and detailed characterization of viability groups. Initially, germination and tetrazolium tests were conducted according to the Rules for Seed Testing for Araucaria spp. After that, more practical methods for carrying out the tetrazolium test were evaluated, without pre-wetting of the seeds and using excised embryos immersed directly in the tetrazolium solution; three concentrations of the solution (0.075, 0.2, and 0.5%), three staining periods (1, 2, and 4 hours), and two staining temperatures (30 and 40°C) were tested. The means obtained for each conducted test were compared by the Scott-Knott test, at 1% probability. The tetrazolium test may be effectively used through the excision of araucaria embryos and the direct immersion in the tetrazolium solution using the following combinations of concentration/period/temperature: 0.5%/2 hours/30°C, 0.075%/4 hours/40°C, or 0.5%/2 hours/40°C, allowing the classification of the seeds into two different viability groups (viable and unviable).

show abstract

“…In addition to the seeds preconditioning and preparation steps, the use of a solution with different concentrations of tetrazolium, the staining time and temperature, and the proper interpretation of the seeds color are also crucial steps to ensure successful testing (Abbade and Takaki, 2014).…”

Section: Tetrazolium Testmentioning

confidence: 99%

“…The information provided by this test may help the nursery technician make safer decisions on the discard of lots with unsuitable quality for seedlings production (Azerêdo et al, 2011), or estimate the feasibility of seeds that require long time to germinate (Pinto et al, 2008), or determine the viability of seeds that have short shelf life during storage (Abbade and Takaki, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…However, some research efforts have been conducted in order to adjust the method, such as for seeds of Peltophorum dubium ("canafístula") (Oliveira et al, 2005), Lafoensia pacari ("mangaba-brava") (Mendonça et al, 2006), Gleditschia amorphoides ("sucará") (Fogaça et al, 2006), Schizolobium parahyba ("guapuruvu") (Ferreira et al, 2007), Poecilanthe parviflora ("coração-de-negro") (Pinto et al, 2008), Parkia velutina ("visgueiro") (Mendes et al, 2009), Eugenia pleurantha ("pitanga-do-mato") (Masetto et al, 2009) and in seeds under storage, such as Acca sellowiana ("Brazilian guava") (Sarmento et al, 2013) and Tabebuia roseoalba ("white ipe") (Abbade and Takaki, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

et al. 2014

View full text Add to dashboard Cite

-The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.Index terms: viability test, surinam cherry, native fruit.Adequação da metodologia do teste de tetrazólio para estimativa da viabilidade de sementes de Eugenia uniflora L. submetidas ao armazenamento RESUMO -O estudo teve como objetivo adequar a metodologia do teste de tetrazólio para estimativa da viabilidade de sementes de Eugenia uniflora (pitanga). As sementes colhidas em setembro de 2012 foram divididas em quatro lotes: recém-colhidas (lote I), armazenadas em sacos plásticos em refrigerador a 10 °C durante 15 (lote II); 30 (lote III) e 45 dias (lote IV). As sementes recém-colhidas foram submetidas aos pré-condicionamentos: embebição direta em água e em rolo de papel umedecido, ambos por 24 horas a 25 ºC e, em seguida, submetidas ou não ao corte longitudinal. Posteriormente, as sementes foram imersas em solução de tetrazólio a 0,1% por quatro horas a 30 ºC. O método de embebição e preparo mais adequados foram aplicados aos lotes, utilizando-se três concentrações de tetrazólio: 0,1; 0,5 e 1,0% e quatro tempos de coloração (2, 4, 6 e 8 horas) a 30 ºC. Os resultados obtidos pelo teste de tetrazólio foram comparados com os de germinação. A imersão direta das sementes em água por 24 horas a 25 ºC, seguida do corte longitudinal, apresentou eficiência no pré-condicionamento de sementes de pitanga. O teste de tetrazólio a 0,5% por 2 horas a 30 ºC pode ser utilizado para estimar a viabilidade tanto de sementes recém-colhidas como armazenadas.Termos para indexação: teste de viabilidade, pitanga, frutífera nativa.

show abstract

Teste de tetrazólio para avaliação da qualidade de sementes de Tabebuia roseoalba (Ridl.) Sandwith - bignoniaceae, submetidas ao armazenamento

Abstract: TETRAZOLIUM TEST FOR QUALITY ASSESSMENT OF SEEDS OF Tabebuia roseoalba (Ridl.) Sandwith BIGNONIACEAE, SUBJECTED TO STORAGE

Cited by 36 publications

References 11 publications

Viability and vigour in seeds of Poincianella pyramidalis (Tul.) L. P. Queiroz by tetrazolium test

Viability and vigour in seeds of Poincianella pyramidalis (Tul.) L. P. Queiroz by tetrazolium test

Critérios para condução do teste de tetrazólio em sementes de araucária

Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

Contact Info

Product

Resources

About