Testican-1 is dispensable for mouse development

Röll, Sandra; Seul, Judith; Paulsson, Mats; Hartmann, Ursula

doi:10.1016/j.matbio.2006.05.004

Cited by 34 publications

(26 citation statements)

References 27 publications

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“…Interestingly, NTG significantly down-regulated testican-1, a multidomain proteoglycan that was first isolated from human seminal plasma [39], and expressed differentially in the human prostate, heart, cartilage and blood with the highest concentrations in the brain [40]. Testican contains calcium ion binding and three potential inhibitory domains (N-terminal region, thyroglobulin and follistatin-like domain), which are targeted towards three different classes of proteolytic enzymes, viz., metalloproteinase, cysteine and serine proteases.…”

Section: Discussionmentioning

confidence: 99%

Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin

Krishnatry¹,

Brazeau²,

Fung³

2010

Nitric Oxide

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Although nitroglycerin (NTG) is effective for the acute relief in coronary ischemic diseases, its longterm benefits in mortality and morbidity have been questioned. The possibility has been raised that NTG may increase the activity of matrix metalloproteinases (MMP), which could lead to disruption and dislodging of atherosclerotic plaques. This study examined the broad effects of acute NTG exposure on the expression and activity of genes encoding MMP-9, as well as an array of ECM and adhesion molecules in THP-1 human macrophages. Gene array studies identified that while NTG exposure (100 µM, 48 hours) did not significantly increase MMP-9 gene expression, genes encoding testican-1, integrin α-1, thrombospondin-3, fibronectin-1 and MMP-26 were significantly downregulated. On the other hand, genes encoding catenin β-1 and vascular cell adhesion molecule-1 were up-regulated. Real-time PCR studies confirmed significant down-regulation of testican-1 gene expression, but its protein expression was not significantly altered. NTG exposure, caused a significant increase in total MMP-9 protein expression (1.96-fold) and active MMP-9 (3.7-fold) concentrations. Recombinant MMP-9 was significantly activated by NTG and its dinitrate metabolites, indicating post-translation modification of this protein by organic nitrates. These results indicate that NTG exposure could broadly affect the gene expression and activity of proteases that govern the ECM cascade, thereby potentially altering atherosclerotic plaque stability.

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Section: Discussionmentioning

confidence: 99%

Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin

Krishnatry¹,

Brazeau²,

Fung³

2010

Nitric Oxide

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“…Abrogation of expression of testican-1 in mice did not reveal any demonstrable phenotypic differences during development or in adult mice (Roll et al, 2006). Histological examination of the brains of testican-1 null mice (the site of highest testican-1 expression) exhibited no significant differences from that of wild-type mice.…”

Section: Testican/spockmentioning

confidence: 91%

“…Histological examination of the brains of testican-1 null mice (the site of highest testican-1 expression) exhibited no significant differences from that of wild-type mice. Although increases in levels of testican-2 and -3 were not detected in the absence of testican-1, compensation was likely conferred for the lack of testican-1 by these related family members (Roll et al, 2006). Of note, abrogation of many matricellular proteins, have resulted in relatively mild phenotypes that are more pronounced in response to injury or challenge.…”

Section: Testican/spockmentioning

confidence: 97%

Diverse biological functions of the SPARC family of proteins

Bradshaw

2012

The International Journal of Biochemistry & Cell Biology

224

165

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The SPARC family of proteins represents a diverse group of proteins that modulate cell interaction with the extracellular milieu. The eight members of the SPARC protein family are modular in nature. Each shares a follistatin-like domain and an extracellular calcium binding E–F hand motif. In addition, each family member is secreted into the extracellular space. Some of the shared activities of this family include, regulation of extracellular matrix assembly and deposition, counter-adhesion, effects on extracellular protease activity, and modulation of growth factor/cytokine signaling pathways. Recently, several SPARC family members have been implicated in human disease pathogenesis. This review discusses recent advances in the understanding of the functional roles of the SPARC family of proteins in development and disease.

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“…Considering the known functional redundancy of SPARC family of proteins in the brain and the entailing difficulties in using traditional knock-out approach to delineate functional significance of SPARC family members (Gilmour et al, 1998; McKinnon et al, 2000; Roll et al, 2006), this approach provides a useful model to analyze SC1 function in a cell type specific manner in the brain.…”

Section: Discussionmentioning

confidence: 99%

A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system

Weimer

Stanco

Cheng

et al. 2008

Glia

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Extracellular matrix associated Sparc-like 1 (SC1/SPARCL1) can influence the function of astroglial cells in the developing and mature central nervous system (CNS). To examine SC1’s significance in the CNS, we generated a BAC transgenic mouse model in which Sc1 is expressed in radial glia and their astrocyte derivatives using the astroglial-specific Blbp (Brain-lipid binding protein; [Feng et al., (1994) Neuron 12:895–908]) regulatory elements. Characterization of these Blbf-Sc1 transgenic mice show elevated Sc1 transcript and protein in an astroglial selective pattern throughout the CNS. This model provides a novel in vivo system for evaluating the role of SC1 in brain development and function, in general, and for understanding SC1’s significance in the fate and function of astroglial cells, in particular.

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Testican-1 is dispensable for mouse development

Cited by 34 publications

References 27 publications

Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin

Broad regulation of matrix and adhesion molecules in THP-1 human macrophages by nitroglycerin

Diverse biological functions of the SPARC family of proteins

A BAC transgenic mouse model to analyze the function of astroglial SPARCL1 (SC1) in the central nervous system

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