Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Kumari, Deepa; Nair, Neena; Bedwal, R. S.

doi:10.3109/19396368.2011.584500

Cited by 29 publications

(19 citation statements)

References 67 publications

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“…Previous studies have shown similar results, suggesting that Zn 2+ can inhibit maturation of caspase 3, an apoptotic protease, which acts upstream of the endonuclease in apoptotic cell death [22,50]. In addition, Kumari et al (2011) [51] showed that prepubertal dietary Zn 2+ deficiency induced apoptotic changes in the testes of Wistar rats. Our results indicate that Cd 2+ induces apoptosis via the mitochondrial pathway, and Zn 2+ supplementation inhibits this induced cell death process.…”

Section: Discussionmentioning

confidence: 64%

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

Rahman

Ukiana

Uson-Lopez

et al. 2017

Chemico-Biological Interactions

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Cadmium (Cd) is a well studied inducer of cellular necrosis and apoptosis. Zinc (Zn) is known to inhibit apoptosis induced by toxicants including Cd both in vitro and in vivo. The mechanism of Zn-mediated protection from Cd-induced cytotoxicity is not established. In this study, we aimed to understand the effects of Zn on Cd-induced cytotoxicity and apoptosis using PC12 cells. Cell viability and DNA fragmentation assays in PC12 cells exposed to Cd and/or Zn revealed that Cd (5 and 10 μmol/L) alone induced significant cell death, and co-exposure to Zn (5, 10, and 100 μmol/L) for 48 h had a protective effect. Assessment of intracellular free sulfhydryl levels and lactate dehydrogenase activity suggested that Cd (10 μmol/L) induced oxidative stress and disrupted cell membrane integrity. Addition of Zn (10 and 100 μmol/L) reduced Cd-mediated cytotoxicity. Changes in expression of the apoptotic factors Bax, Bcl-2, Bcl-x, and cytochrome c were measured via western blot and expression of caspase 9 was detected via reverse transcriptase polymerase chain reaction. Western blots showed that Zn (10 and 100 μmol/L) suppressed Cd-induced apoptosis (10 μmol/L) by reducing cytochrome c release into the cytosol, and downregulating the proapoptotic protein, Bax. In addition, expression of caspase 9 was lower in Cd (5 μmol/L)-treated PC12 cells when co-treated with Zn (2 and 5 μmol/L). These findings suggest that the effective inhibition of Cd-induced apoptosis in PC12 cells by Zn might be due to suppression of mitochondrial apoptosis pathway and inhibition of Cd-induced production of reactive oxygen species.

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Section: Discussionmentioning

confidence: 64%

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

Rahman

Ukiana

Uson-Lopez

et al. 2017

Chemico-Biological Interactions

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“…Zn deficiency enhances apoptotic processes in the testis and negatively affects semen quality by reducing the number and motility of sperm (Kumari et al 2011). Research conducted by Koehler et al (2009) does not show a statistically significant increase in the concentration of TT and FT in young men supplemented with Zn (Koehler et al 2009).…”

Section: Bioelementsmentioning

confidence: 89%

Analysis of the relationship between the blood concentration of several metals, macro- and micronutrients and endocrine disorders associated with male aging

Rotter

Kosik-Bogacka

Dołęgowska

et al. 2015

Environ Geochem Health

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Beyond 30 years of age, men experience a decline in the production of testosterone, yet only a few develop late-onset hypogonadism. This study was designed to determine the relationship between blood concentrations of metals, macro- and micronutrients and age-related testosterone deficiency and associated hormonal changes in aging men. The research involved 313 men aged 50-75 years. We used ELISA to determine the concentrations of total testosterone (TT), free testosterone (FT), estradiol (E2), dehydroepiandrosterone sulfate (DHEAS) and sex hormone-binding globulin (SHBG). We calculated free androgen index (FAI). With the use of emission spectrometry in inductively coupled argon plasma, we determined the whole-blood concentrations of lead (Pb), cadmium (Cd), mercury (Hg), arsenic (As) and tungsten (W), as well as serum concentrations of magnesium (Mg), iron (Fe), calcium (Ca), copper (Cu), zinc (Zn), selenium (Se), chromium (Cr), manganese (Mn) and molybdenum (Mo). The study showed no relationship between TT and FT and the concentrations of metals. Men with TT deficiency had significantly lower concentrations of Mg and Fe and increased Mn. Men with FT deficiency had higher W and Cr levels and lower Fe. Assessing the correlation between the concentrations of hormones, SHBG and FAI, and the concentration of metals and macro- and microelements in the blood of the men, we found positive correlations between the concentrations of TT-Mg, TT-Fe, TT-Mo, FT-Fe, E2-As, SHBG-Mn, FAI-W, FAI-As, FAI-Zn and FAI-Ca, and negative correlations between the concentrations of TT-Mn, FT-Cd, FT-Cr, E2-Hg, E2-Cr, SHBG-W, SHBG-As, SHBG-Zn, SHBG-Ca, FAI-Pb and FAI-Mn. Positive correlations between As and E2 and between As and FAI may suggest a lack of association between this metal and hypogonadism in people not exposed to excess As levels. Our research indicates a positive relationship between the concentrations of Mg, Fe and Zn and endocrine system in aging men, in contrast to Mn and Cr. Toxic metals (Cd, Pb) seemed to negatively affect the level of bioavailable testosterone. In persons not exposed to As, As does not contribute late-onset hypogonadism. Heavy metals (Pb, Cd, Hg and W) may contribute to a lower concentration of DHEAS. The role of W in men with LOH was found to be ambiguous, as on the one hand its concentration was higher in men with FT deficiency, and on the other hand it positively correlated with FAI, which in turn indirectly indicates testosterone availability. Copper and selenium do not seem to play any significant role in the occurrence of TT deficiency in aging men.

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“…RNAs encoding various members of the metallothionein family, e.g., MT1E, MT1M, MT1F, and MT1G, are distinctively abundant in seminal fluid (Suzuki et al 1994). These proteins are able to bind zinc, an ion important for sperm motility (Lopez Rodriguez et al 2013), genome integrity (reviewed in Bjorndahl and Kvist 2011) and likely fertilization (Sorensen et al 1999;Kumari et al 2011). Only 14 seminal fluid transcripts that are not observered to be of testicular origin are observed as present in sperm.…”

Section: Semen Rna Profilementioning

confidence: 92%

The protein and transcript profiles of human semen

2015

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The increasing use of "-omics" (genomic, transcriptomic, proteomic, epigenomic, and metabolomic) high-throughput measurement technologies over the past decade is beginning to reveal the complexity of human biology and physiology through the interactions of DNA, RNA, related proteins and small molecules. In reproductive medicine, the majority of this work, has thus far focused on the female factors, e.g., the oocyte, since they provide both the environment and the majority of elements required for embryogenesis. State-of-the-art sequencing and computational analyses have enabled a deeper understanding of the underlying components. Contrary to being simply a silent delivery vehicle to the oocyte of the packaged male DNA, sperm provide both a specific epigenetically marked genome together with a complex population of RNAs and proteins that are crucial for early embryogenesis. In addition to the sperm, seminal fluid appears to serve multiple roles providing a supplementary series of components that allow the sperm to successfully reach and fertilize the oocyte and prepare the female immune system to tolerate the semiallosteric embryo. A global analysis and review of what is presently known regarding the unique role of each component of the male factor and their associated interactions begins to shed light on this emergent field.

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Testicular apoptosis after dietary zinc deficiency: Ultrastructural and TUNEL studies

Cited by 29 publications

References 67 publications

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

Analysis of the relationship between the blood concentration of several metals, macro- and micronutrients and endocrine disorders associated with male aging

The protein and transcript profiles of human semen

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