Testicular development and establishment of spermatogenesis in Nili-Ravi buffalo bulls

Ahmad, Nasim; Umair, Saleh; Shahab, Muhammad; Arslan, Muhammad

doi:10.1016/j.theriogenology.2009.06.034

Cited by 28 publications

(23 citation statements)

References 15 publications

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“…The relationship between age and body weight throughout the study period was significant (r=0.91). This finding is consistent with the earlier work in Holstein bulls (r=0.96; Values with different letters within each row differ significantly (P<0.05) NAR normal apical ridge, PMI plasma membrane integrity Coulter and Foote 1977), Wagyu bulls (r=0.94; Sosa et al 2002), and buffalo bulls (r=0.99; Ahmad et al 2010). It appears that the body weight is lower in Sahiwal bulls as compared to beef (Silva-Mena 1997), dairy (Devkota et al 2008), and even buffalo bulls (Ahmad et al 2010) at the corresponding ages.…”

Section: Discussionsupporting

confidence: 92%

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Relationship of age to body weight, scrotal circumference, testicular ultrasonograms, and semen quality in Sahiwal bulls

Ahmad

Naseer

et al. 2010

Trop Anim Health Prod

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The objective of the present study was to determine the relationship of age to body weight (BW), scrotal circumference (SC), number of pixels of testicular ultrasonograms (NP), and semen quality in Sahiwal bulls. The study was based on 128 Sahiwal bulls of different age groups (from 0 to >100 months of age). Bulls were evaluated for SC, BW, and NP. Semen was evaluated once a week for five consecutive weeks from regularly collected donor bulls (n = 86) ranging in age from 25-30 to >100 months. Ejaculate volume, sperm motility, sperm concentration, sperm morphology, percent live sperms, sperm plasma membrane integrity, and normal acrosome were compared among different age groups. Mean SC and BW increased (P < 0.05) in a curvilinear manner from birth to >100 months of age. Mean NP of testicular ultrasonograms increased (P < 0.05) from 0 to 24 months and then plateaued until >100 months of age. Body weight, SC, and NP were positively correlated with age from birth until >100 months (r = 0.91, 0.87, and 0.40, respectively). Ejaculate volume (5.7 ± 0.2 vs. 4.6 ± 0.09 ml) and sperm concentration (1,281.6 ± 17.7 vs. 1,115.8 ± 55.9 × 10(6)/ml) increased (P < 0.05) in mature bulls compared to younger ones. However, motility (68.6 ± 0.3%), plasma membrane integrity (50.8 ± 1.0%), and normal acrosome (74.8 ± 0.5%) remained insignificant due to age. In six of eight age groups studied, morphological abnormalities were well within the range (18.1 ± 0.3%). In conclusion, the BW, SC, and NP of testicular ultrasonograms, ejaculate volume, and concentration increased with age. Moreover, semen quality is fairly independent of age except volume and concentration in Sahiwal bulls.

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Section: Discussionsupporting

confidence: 92%

“…The information on body and testicular development has been well studied in dairy bulls (Coulter and Foote 1977), beef bulls (Sosa et al 2002), and buffalo bulls (Ahmad et al 2010). However, information on zebu bulls is generally scarce and particularly with Sahiwal bulls.…”

Section: Introductionmentioning

confidence: 99%

Relationship of age to body weight, scrotal circumference, testicular ultrasonograms, and semen quality in Sahiwal bulls

Ahmad

Naseer

et al. 2010

Trop Anim Health Prod

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“…Photomicrograph, hematoxylin-eosin method.). Although spermatids were present in high numbers at 18 months in Nili-Ravi buffalo testes [15], spermatozoa were not present in the tubule lumens analyzed here.…”

Section: Resultsmentioning

confidence: 99%

The Correlation between Age, Body Weight and Testicular Parameters in Murrah Buffalo Bulls Raised in Brazil

Luz

Santos

Andrighetto

et al. 2013

Journal of Reproduction and Development

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Buffalo are an economically important source for meat and milk production, especially in Brazil. However, important aspects of their biology remain unknown thus far. Herein, we describe the reproductive characteristics of male Murrah buffalo (Bubalus bubalis) raised under extensive management conditions by applying biometrics associated with testicular weight. We analyzed seven males, divided into two groups: G1, which consisted of four 18-month-old animals, and G2, which consisted of three 24-month-old animals. Testicular development occurs slowly in Murrah buffalo, suggesting a delay of sexual maturity. The biometric testicular parameters analyzed were scrotal circumference, testicular weight, testicular length, testicular width, testicular thickness and testicular circumference. Our data indicate strong correlations between SC, age and body weight, and additional significant relationships were identified between body weight, age and other testicular parameters. Thus, these parameters are suitable indicators when selecting bulls for breeding purposes.

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“…In this study, the grafts recovered from intact recipients showed the presence of elongated spermatids, indicating that complete germ cell differentiation is possible in grafts collected from intact recipients 24 weeks after grafting. Elongated spermatids are not detected in buffalo testis until 72-84 weeks of age (Rana & Bilaspuri 2004, Ahmad et al 2010. This complete differentiation of germ cells in intact recipients can again be attributed to the elevated testosterone level compared with castrated recipients.…”

Section: Discussionmentioning

confidence: 95%

Gonadal status of male recipient mice influences germ cell development in immature buffalo testis tissue xenograft

Reddy

Mahla²,

Thathi³

et al. 2012

Reproduction

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Growth and development of immature testis xenograft from various domestic mammals has been shown in mouse recipients; however, buffalo testis xenografts have not been reported to date. In this study, small fragments of testis tissue from 8-week-old buffalo calves were implanted subcutaneously onto the back of immunodeficient male mouse recipients, which were either castrated or left intact (non-castrated). The xenografts were retrieved and analyzed 12 and 24 weeks later. The grafted tissue survived and grew in both types of recipient with a significant increase in weight and seminiferous tubule diameter. Recovery of grafts from intact recipients 24 weeks post-grafting was significantly lower than that from the castrated recipients. Seminal vesicle indices and serum testosterone levels were lower in castrated recipients at both collection time points in comparison to the intact recipients and non-grafted intact mouse controls. Pachytene spermatocytes were the most advanced germ cells observed in grafts recovered from castrated recipients 24 weeks post-grafting. Complete spermatogenesis, as indicated by the presence of elongated spermatids, was present only in grafts from intact recipients collected 24 weeks post-grafting. However, significant number of germ cells with DNA damage was also detected in these grafts as indicated by TUNEL assay. The complete germ cell differentiation in xenografts from intact recipients may be attributed to efficient Sertoli cell maturation. These results suggest that germ cell differentiation in buffalo testis xenograft can be completed by altering the recipient gonadal status.

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Testicular development and establishment of spermatogenesis in Nili-Ravi buffalo bulls

Cited by 28 publications

References 15 publications

Relationship of age to body weight, scrotal circumference, testicular ultrasonograms, and semen quality in Sahiwal bulls

Relationship of age to body weight, scrotal circumference, testicular ultrasonograms, and semen quality in Sahiwal bulls

The Correlation between Age, Body Weight and Testicular Parameters in Murrah Buffalo Bulls Raised in Brazil

Gonadal status of male recipient mice influences germ cell development in immature buffalo testis tissue xenograft

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