Testicular echotexture and seminal quality of young Montana Tropical Compound bulls classified as sound and unsound for breeding

Pinho, Rogério Oliveira; Siqueira, Jeanne Broch; Chaya, Alberto Yukio; Neto, Tamires Miranda; Martins, Leonardo Franco; Guimarães, Simone Eliza Facioni; Guimarães, José Domingos

doi:10.1590/s1516-35982012000800023

Cited by 5 publications

(4 citation statements)

References 12 publications

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“…(2015) showed that mean testicular heterogeneity was positively correlated with the total spermatozoal output. However, other researchers did not find a correlation between pixel intensity and sperm production or semen quality in dogs (Dias et al., 2017; Pinho et al., 2012; Tomlinson et al., 2017). Many researchers have reported that testicular echogenicity increases during sexual development directly with seminiferous tubules growth (Camela et al., 2018; Hamm & Fobbe, 1995; Pozor et al., 2017; Silva et al., 2012).…”

Section: Discussionmentioning

confidence: 92%

Salmon oil supplementation in dogs affects the blood flow of testicular arteries

Santos

Milani

Zucchini

et al. 2021

Reprod Domestic Animals

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The administration of fish oils is known to cause changes in several reproductive parameters of domestic animals. The ingestion of polyunsaturated fatty acids (PUFAs) of the omega‐3 family, such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has been described and correlated with changes in the semen quality, testosterone levels and male fertility. Nevertheless, few studies monitored and registered effects after ceasing supplementation. In the present study, we monitored the Doppler velocimetric and ultrasonographic parameters of nine dogs' testis for 90 days (D90) checking the effect of salmon oil supplementation, and monitoring continued for 60 days more, after ceasing supplementation (D150). Ultrasonographic evaluations comprised determining the Doppler velocimetric parameters, testicular and epididymal volume, and testicular echotexture. Peak systolic velocity (PSV) as well as final diastolic velocity (EDV) in the supratesticular arteries (STA), and marginal artery (MA) increased during the period of treatment and kept that level up to D150. There was no difference between the fish‐oil supplementation period and the unsupplemented one regarding the testicular and epididymal volume and echogenicity and heterogeneity characteristics. A negative correlation was found between heterogeneity of testis and sperm production (r = −.41, p = .008). Doppler velocimetry indices were affected by the supplementation, leading to an increase in testicular blood flow.

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Section: Discussionmentioning

confidence: 92%

Salmon oil supplementation in dogs affects the blood flow of testicular arteries

Santos

Milani

Zucchini

et al. 2021

Reprod Domestic Animals

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“…Moreover, Kastelic et al (2001) reported an intensity of 158.5 while working with Angus cattle, and Carmo et al (2012) analysed Guzerat cattle that were 21-24, 24.1-27, and 27.1-30 months old and obtained values of 102.3, 112.0, and 127.5, respectively. Pinho et al (2012) worked with Montana cattle, obtaining values of 95.7 and 94.0 for animals that could and could not reproduce, respectively. Finally, Cardilli et al (2012) worked with sexually mature 18 month old Nelore bulls and obtained a value of 115.3.…”

Section: Resultsmentioning

confidence: 99%

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

Dias

Faria

Fernandes

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm 2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.Key words: testicular parenchyma, morphometry, ultrasonography, bovine. RESUMEN.El objetivo de este estudio fue evaluar la ecotextura testicular de los toros Nelore y correlacionarlo con los parámetros de espermatogénesis. Para este propósito, 41 toros de edades de 3-5 años y con pesos corporales en el intervalo de 510,2 ± 21,61 kg fueron seleccionados para el estudio. Los toros se sometieron a ecografía, utilizando un dispositivo portátil junto con un transductor lineal a una frecuencia de 7,5 MHz. Inmediatamente después, los animales fueron castrados, y el tejido testicular se trató con Karnovsky fijador. Los fragmentos testiculares se incluyeron en metacrilato de glicol y se tiñeron con una solución de borato de sodio-azul de toluidina al 1%. La intensidad media de pixeles en el parénquima testicular se determinó para secciones de superficie de 1.600 mm 2 . Los El área entre los dos testículos derecho e izquierdo dieron lugar a la intensidad media de los pixeles de 105,22 ± 13,58, 107,95 ± 12,82 y 106,59 ± 11,43, respectivamente. El coeficiente de eficiencia de la mitosis espermatogonial fue 19,74; Índice meiótico fue 2,63, y el rendimiento global del espermatogénesis alcanzó el 52,0. No se observó correlación (P>0,05) entre ecotextura testicular y cualquiera de los parámetros morfométricos medidos. Estos resultados sugieren que ecotextura testicular no es un método viable para el análisis indirecto de la población celular en el epitelio seminífero, el rendimiento de la espermatogénesis intrínseco, o el índice de células de Sertoli en toros Nelore.

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“…Testicular echogenicity was obtained from the grayscale of pixels (0–255), where the minimum numbering (0) equaled the black tone pixel and the maximum numbering (255) equaled the white tone pixel, as previously described [ 46 , 47 ]. The analyses were performed in the sagittal (longitudinal) plane.…”

Section: Methodsmentioning

confidence: 99%

Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)

Benchimol

Santos

Lopes³

et al. 2023

Viruses

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During the Zika virus (ZIKV) outbreak and after evidence of its sexual transmission was obtained, concerns arose about the impact of the adverse effects of ZIKV infection on human fertility. In this study, we evaluated the clinical-laboratory aspects and testicular histopathological patterns of pubertal squirrel monkeys (Saimiri collinsi) infected with ZIKV, analyzing the effects at different stages of infection. The susceptibility of S. collinsi to ZIKV infection was confirmed by laboratory tests, which detected viremia (mean 1.63 × 106 RNA copies/µL) and IgM antibody induction. Reduced fecal testosterone levels, severe testicular atrophy and prolonged orchitis were observed throughout the experiment by ultrasound. At 21 dpi, testicular damage associated with ZIKV was confirmed by histopathological and immunohistochemical (IHC) analyses. Tubular retraction, the degeneration and necrosis of somatic and germ cells in the seminiferous tubules, the proliferation of interstitial cells and an inflammatory infiltrate were observed. ZIKV antigen was identified in the same cells where tissue injuries were observed. In conclusion, squirrel monkeys were found to be susceptible to the Asian variant of ZIKV, and this model enabled the identification of multifocal lesions in the seminiferous tubules of the infected group evaluated. These findings may suggest an impact of ZIKV infection on male fertility.

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Testicular echotexture and seminal quality of young Montana Tropical Compound bulls classified as sound and unsound for breeding

Cited by 5 publications

References 12 publications

Salmon oil supplementation in dogs affects the blood flow of testicular arteries

Salmon oil supplementation in dogs affects the blood flow of testicular arteries

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

Zika Virus Infection Damages the Testes in Pubertal Common Squirrel Monkeys (Saimiri collinsi)

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