Testicular Histomorphometric Evaluation of Zebu Bull Breeds

Andreussi, Paulo Antonio Terrabuio; Faria, F. J. C.; Fernandes, C. A. C.; Santos, Marcelo Diniz dos; Silva, Juliana Corrêa Borges

doi:10.1590/s1516-8913201402262

Cited by 7 publications

(8 citation statements)

References 26 publications

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“…In the present study, the values observed for the volumetric proportions of the testicular parenchyma constituents were in line with those observed by Andreussi et al [1], with 80.9% of the testicular parenchyma occupied by seminiferous tubules. However, this result differed from the overall average of all other breeds as, according to Andreussi et al [1], animals of the Nelore breed had a higher volume of seminiferous tubules compared to all other Zebu breeds studied.…”

Section: Discussionsupporting

confidence: 93%

“…The gonadosomatic index in the Gir breed found in the present study (0.12%) was similar to that observed in other studies performed on zebuine cattle. Andreussi et al [1] also found a gonadosomatic index of 0.12% in Gir animals, with an average of 0.13% in the Nelore, Nelore mocho, Gir, Tabapuã, and Guzerá breeds. In young taurine animals of the Shorthorn breed, Swierstra [27] obtained an GSI of 0.8%.…”

Section: Discussionmentioning

confidence: 90%

“…Moreover, the dominant taurine genetics were challenged by huge problems with endo-and ecto-parasites, causing great concern to rural producers. With this scenario came the need to import cattle of Indian origin, which is rustic and better adapted to the tropical environment [1] [2].…”

Section: Introductionmentioning

confidence: 99%

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Functional Morphology of the Spermatogenesis of Gir Bulls

Silva¹,

Faria²,

Furtado³

et al. 2020

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The purpose of this study was to evaluate the functional morphology of the spermatogenesis of Gir bulls, with emphasis on the testicular biometry and testicular parenchyma histomorphometry. Testicular fragments from eight Gir bulls were used. The fragments were fixed by tissue perfusion with Karnovsck's solution, and inclusion was subsequently performed using glycol methacrylate. Histological sections of 4 μm were made and stained with a 1% toluidine blue-sodium borate solution. The mean age of the bulls was 8.0 ± 1.3 years and the mean body weight was 467.5 ± 26.7 kg. Testicular weight was 289.2 ± 30.5 g on average, and the average gonadosomatic index was 0.12% ± 0.02%. The seminiferous tubules, Leydig cells, stroma, and intertubular tissue composed 80.9% ± 1.7%, 5.4% ± 1.3%, 13.7% ± 1.1%, and 19.1% ± 1.7% of the total testicular weight, respectively. The mean length of the seminiferous tubule per gram of testicle was 17 ± 0.8 m. Losses during spermatogonia mitosis averaged 75.5% ± 3.22%, while losses were 30.6% ± 8.17% during the meiotic phase, which resulted in an average total loss of 81.49% ± 2.58% of cells during the entire process of spermatogenesis. The average daily sperm production per gram of parenchyma was 28.0 × 10 6 cells. It was concluded that the histometric measurements of testicular parameters in the Gir breed are within the averages reported for other zebu cattle breeds.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 90%

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Functional Morphology of the Spermatogenesis of Gir Bulls

Silva¹,

Faria²,

Furtado³

et al. 2020

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“…In addition, no significant differences were observed in pixel intensities between the right and left testicles, reinforcing observations by Aravindakshan et al (2000), Cardilli et al (2009), andPinho et al (2013). The observed homogeneity has already been reported in previous studies, in which fragments extracted from different sections of the testicle presented morphometric counts that were not significantly different from each other (Andreussi et al 2014).…”

Section: Resultssupporting

confidence: 86%

“…Significant differences in daily sperm production have been observed in sexually mature Zebu bulls (Andreussi et al 2014), therefore, a new method to track these differences would significantly improve breeding options.…”

Section: Introductionmentioning

confidence: 99%

Testicular echotexture is not a viable method to indirectly evaluate the spermatogenic parameters in Nelore bulls

Dias

Faria

Fernandes

et al. 2017

Austral j. vet. sci.

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ABSTRACT. The objective of this study was to evaluate the testicular echotexture of Nelore bulls and to correlate it with spermatogenic parameters. Forty one bulls aged 3-5 years, with body weight of 510.2 ± 21.61 kg were selected for the study. The animals were subjected to ultrasonography, using a portable device coupled with a linear transducer at a frequency of 7.5 MHz. Immediately afterwards they were castrated and the testicular tissue was treated with Karnovsky fixative. Next, the fragments were embedded in glycol methacrylate and stained with toluidine blue-1% sodium borate solution. The average pixel intensity in the testicular parenchyma was determined for 1,600-mm 2 surface sections. The right and left testicles and the area between the two testicles resulted in average pixel intensities of 105.22 ± 13.58, 107.95 ± 12.82, and 106.59 ± 11.43, respectively. The efficiency coefficient of spermatogonial mitosis was 19.74; meiotic index was 2.63, and overall yield of spermatogenesis was 52.0. No correlation (P>0.05) was observed between testicular echotexture and any of the measured morphometric parameters. These results suggest that testicular echotexture was not a viable method for the indirect analysis of cellular population in the seminiferous epithelium, intrinsic spermatogenic performance, or the Sertoli cell index in Nelore bulls.Key words: testicular parenchyma, morphometry, ultrasonography, bovine. RESUMEN.El objetivo de este estudio fue evaluar la ecotextura testicular de los toros Nelore y correlacionarlo con los parámetros de espermatogénesis. Para este propósito, 41 toros de edades de 3-5 años y con pesos corporales en el intervalo de 510,2 ± 21,61 kg fueron seleccionados para el estudio. Los toros se sometieron a ecografía, utilizando un dispositivo portátil junto con un transductor lineal a una frecuencia de 7,5 MHz. Inmediatamente después, los animales fueron castrados, y el tejido testicular se trató con Karnovsky fijador. Los fragmentos testiculares se incluyeron en metacrilato de glicol y se tiñeron con una solución de borato de sodio-azul de toluidina al 1%. La intensidad media de pixeles en el parénquima testicular se determinó para secciones de superficie de 1.600 mm 2 . Los El área entre los dos testículos derecho e izquierdo dieron lugar a la intensidad media de los pixeles de 105,22 ± 13,58, 107,95 ± 12,82 y 106,59 ± 11,43, respectivamente. El coeficiente de eficiencia de la mitosis espermatogonial fue 19,74; Índice meiótico fue 2,63, y el rendimiento global del espermatogénesis alcanzó el 52,0. No se observó correlación (P>0,05) entre ecotextura testicular y cualquiera de los parámetros morfométricos medidos. Estos resultados sugieren que ecotextura testicular no es un método viable para el análisis indirecto de la población celular en el epitelio seminífero, el rendimiento de la espermatogénesis intrínseco, o el índice de células de Sertoli en toros Nelore.

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