Testicular Sperm Extraction and Intracytoplasmic Sperm Injection: A Chance of Fertility in Nonobstructive Azoospermia

Gil-Salom, Manuel; Romero, J.L.; Mı́nguez, Yolanda; Molero, M.D.; Remohı́, José; Pellicer, A.

doi:10.1016/s0022-5347(01)62243-2

Cited by 56 publications

(21 citation statements)

References 17 publications

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“…In NOA, patient age, testicular size, and various molecular markers (e.g., FSH receptor genes) have been determined to be predictive parameters for successful spermatozoa retrieval before assisted reproduction [Gil-Salom et al 1998;Plas et al 1999;Song et al 2000]. These different results may be due to differences in patient selection in terms of FSH concentration and/or testis size before biopsy, degree of spermatogenic defect [Plas et al 1999], number of testicular biopsies, laboratory techniques used for testicular spermatozoa preparation and spermatozoa selection, protocol of ovarian Values in parentheses are percentages.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, testicular tissue cryopreservation is necessary to avoid repeat TESE for subsequent ICSI trials [Nogueira et al 1999], and successful fertilization and pregnancy have been reported using frozen-thawed testicular spermatozoa and testicular tissue [Oates et al 1997;Gianaroli et al 1999]. The fertilization rate using frozen-thawed spermatozoa from men with NOA has been shown to range from 38-70% Gil-Salom et al 1998;Wood et al 2002]. There have been no reports comparing the result of fertilization and pregnancy using fresh and frozen-thawed testicular spermatozoa in each of the NOA subgroups.…”

Section: Introductionmentioning

confidence: 99%

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Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

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This study was performed to assess and compare the outcomes of testicular sperm extraction (TESE)-intracytoplasmic sperm injection (ICSI) using spermatozoa from fresh and frozen testicular tissue from men with subgroups of non-obstructive azoospermia (NOA). A total of 110 cycles of TESE-ICSI were performed. Patients were classified into one of the following NOA subgroups: hypospermatogenesis (HS), maturation arrest (MA), or Sertoli cell-only syndrome (SCO). Laboratory (fertilization, cleavage stage of embryo, and good quality embryo) and clinical (pregnancy, clinical pregnancy, implantation, and delivery) outcomes were assessed. No statistically significant differences were observed in any of the other measured parameters between the three subgroups of NOA. No significant differences in laboratory outcomes were observed between spermatozoa from fresh and frozen testicular spermatozoa; however, statistically significant differences were observed in the pregnancy and implantation rates between groups (p50.05). The outcomes of using spermatozoa retrieved from fresh testicular tissue in each of the three subgroups were also compared; although clinical outcomes showed low results, no significant differences were observed between the three subgroups. Similarly, no significant differences were observed in spermatozoa retrieved from frozen testicular tissue. Once spermatozoa have been successfully obtained, acceptable laboratory outcomes can be achieved for NOA, whether or not the spermatozoa are cryopreserved. However, satisfactory clinical outcomes may be more difficult to achieve as the results showed in each group of fresh and frozen testicular spermatozoa. Therefore, achieving acceptable clinical pregnancy results and efficient cryopreservation of testicular spermatozoa should be considered in patients with NOA.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Park

Lee

Lim

et al. 2015

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…Other studies have been published evaluating fertilization and pregnancy rates after ICSI, comparing fresh and cryopreserved human spermatozoa (10,12,15). However, the different results among the studies published may be due to the fact that some of the studies were published evaluating testicular or epididymal sperm instead of ejaculated sperm.…”

Section: Discussionmentioning

confidence: 99%

“…Even though there is no doubt that cryopreserved spermatozoa can be used to fertilize oocytes by ICSI, more studies comparing the efficacy of ICSI with either fresh or cryopreserved ejaculated spermatozoa from infertile patients should be performed. Also, the majority of studies comparing fresh and cryopreserved sperm have shown results from sperm surgically retrieved (10,12,15).…”

mentioning

confidence: 99%

Fertilization and pregnancy outcome after intracytoplasmic injection with fresh or cryopreserved ejaculated spermatozoa

Borges

Rossi

Freitas

et al. 2007

Fertility and Sterility

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“…In non-obstructive azoospermia, the finding of testicular spermatozoa constitutes a key element predicting treatment success as only half of these men will have sperm. 19 Epididymal sperm extraction is not usually feasible in non-obstructive azoospermic men as sperm are rarely present in epididymal tissue.…”

Section: Discussionmentioning

confidence: 99%

Cryptorchidism and its impact on male fertility: a state of art review of current literature

Chung

Brock

2013

CUAJ

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Cryptorchidism is associated with impairment of germ cell maturation and subsequent infertility in adulthood. The present report details common scenarios of referral of men with prior history of cryptorchidism and orchidopexy seeking advice for infertility, and examines the association between cyrptorchidism and male infertility. The increase in the understanding of the hormonal profiles and patho-physiological changes in germ cell maturation in cryptorchid boys may potentially change our approach and management strategies. Improvement in sperm retrieval techniques and micromanipulation techniques, such as intracytoplasmic sperm injection, has led to excellent fertilization and pregnancy outcomes of treatment cycles.

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Testicular Sperm Extraction and Intracytoplasmic Sperm Injection: A Chance of Fertility in Nonobstructive Azoospermia

Cited by 56 publications

References 17 publications

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Effect of testicular spermatozoa on embryo quality and pregnancy in patients with non-obstructive azoospermia

Fertilization and pregnancy outcome after intracytoplasmic injection with fresh or cryopreserved ejaculated spermatozoa

Cryptorchidism and its impact on male fertility: a state of art review of current literature

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