Testicular tissue oxidative stress in azoospermic patients: Effect of cryopreservation

Abstract

This study evaluated oxidative stress (OS) in human testicular tissue in obstructive (OA) and nonobstructive azoospermia (NOA) before and after cryopreservation. Azoospermic patients were subjected to surgical sperm retrieval (SSR) and cryopreservation. Assay of catalase activity (CAT) and malondialdehyde level (MDA) using colorimetric methods was done. The number of retrieved spermatozoa and the Johnson score were assessed in fresh testicular samples. The study included 21 OA (group A) and 16 positive sperm retrieval (group B, positive NOA). The basal CAT activity in the positive NOA group was significantly higher than that in the OA group. Basal MDA level was also significantly higher in NOA than in OA group. CAT activity and MDA level correlated negatively with the mean number of retrieved spermatozoa. After cryopreservation, there was a significant increase in CAT activity in OA, while there was no significant difference in MDA level in both OA and positive NOA. We concluded that patients with NOA seem to have increased testicular OS compared to OA. The extent of OS correlated negatively with spermatogenesis grade. Testicular tissue of patients with OA seems to withstand OS induced by cryopreservation more than NOA.

Impact of Spermatozoa Cryopreservation on Clinical Outcomes of Intracytoplasmic Sperm Injection in Patients with Azoospermia: A Retrospective Cross-Sectional Study

“…Such results were not observed in OA patients. Our results are supported by the study of Moubasher et al [34]. They showed that catalase activity increased in testicular tissue from OA patients which was not observed in the NOA group, suggesting that testicular tissue from OA patients can withstand the oxidative stress caused by freezing more than NOA [7].…”

Varicoceles in Men With Non-obstructive Azoospermia: The Dilemma to Operate or Not

“…Furthermore, a study by Mubasher et al ( 38 ) indicates high production of reactive oxygen species in a number of azoospermic subjects with varicoceles. Varicocelectomy has the potential to diminish the reactive oxygen species generation and up-regulates the antioxidant activity of seminal plasma ( 39 , 40 ).…”

ОЦЕНКА ОКИСЛИТЕЛЬНОЙ МОДИФИКАЦИИ БЕЛКОВ СПЕРМОПЛАЗМЫ ЖЕРЕБЦОВ (Equus ferus caballus L.) РАЗНОГО ВОЗРАСТА

“…Окислительный стресс (ОС) приводит к повреждению сперматозоидов (1, 2) и снижению качества нативной и криоконсервированной спермы (3)(4)(5). Причиной развития ОС становится чрезмерная продукция активных форм кислорода (АФК), истощение антиоксидантных возможностей организма или совокупность этих факторов.…”