Testicular Torsion-Detorsion- Histological and Biochemical Changes in Rat Testis

Fa, Elshaari; Elfagih, RI; Sheriff, Dhastagir Sultan; If, Barassi

doi:10.4172/2157-7099.1000136

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…Torsion-detorsion was performed by rotating the testicles 720° counterclockwise for 90 minutes. In the experimental and control groups that underwent torsion, detorsion continued for ten days when treatments were applied [2].…”

Section: Induction Of Torsionmentioning

confidence: 99%

“…There are several reasons why the blood may not reach the testicular tissue, which may be due to the lack of oxygen to the tissue that causes hypoxia and the accumulation of large amounts of material [1]. Injury to the testicles due to trauma or torsion requires emergency surgical intervention [2]. Impaired testicular blood flow, following primary trauma or due to surgical procedures, can result in testicular cell death due to depletion of stored energy in the cells and the accumulation of toxic metabolites [3].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats

Masoumi,

Salehi,

Angaji

et al. 2021

GMJ

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Background: Ischemia-reperfusion (I/R) induced by testicular torsion can damage the testicles. In the present study, we assessed the effects of coenzyme Q10 (CoQ10) and diamond nanoparticles on sperm parameters in I/R testes in rats. Materials and Methods: Forty-eight Wistar adult male rats were divided into eight groups: healthy control (Ch), diamond nanoparticle healthy control group (Ch+Dia), CoQ10 healthy control group (Ch+Q10), diamond nanoparticles+CoQ10 healthy control group (Ch+Q10+Dia), torsion/detorsion group (Ct), the Ct group that received diamond nanoparticles (Ct+Dia), the Ct group that received CoQ10 (Ct+Q10), and Ct group that received diamond nanoparticles and CoQ10 (Ct+Q10+Dia). The rats were euthanized, and we collected the semen from the epididymal tissues to evaluate sperm viability, motility, concentration, and morphology parameters. Results: The I/R of the testicles significantly reduced sperm concentration, motility, viability, and altered sperm morphology in the rats. However, the administration of CoQ10 significantly improved sperm parameters in the rats with testicular I/R. Diamond nanoparticles decreased the sperm parameters; however, simultaneous administration of diamond nanoparticles and CoQ10 led to improved sperm parameters. Conclusion: CoQ10 potentially appeared to have protective effects against the long-term side-effects of I/R in testes in rats. Co-administration of diamond nanoparticles with CoQ10 significantly improved sperm parameters and greatly reduced the negative effects of diamond nanoparticles alone. Therefore, green synthesis of nanoparticles with the use of antioxidants such as CoQ10 is recommended. [GMJ.2021;10:e2029]

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Section: Induction Of Torsionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats

Masoumi,

Salehi,

Angaji

et al. 2021

GMJ

View full text Add to dashboard Cite

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Incidence of Low-Grade Testicular Injury in Orchidectomy Specimens Post-testicular Torsion

et al. 2017

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The finding of histopathological features that may represent salvageability of a torted testis occurs relatively rarely. Because of this possibility, appropriate intraoperative steps to check for reperfusion must be undertaken prior to orchidectomy. More evidence for the use of antioxidants and tunica albuginea decompression to improve testes salvage rates is required. The potential for exocrine and endocrine function if partial testicular atrophy occurs and the evidence for contralateral autoimmune testicular damage in pre- and postpubertal males require further investigation.

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Vitamin D3 Prevents the Deleterious Effects of Testicular Torsion on Testis by Targeting miRNA-145 and ADAM17: In Silico and In Vivo Study

et al. 2021

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Testicular torsion (TT) is the most common urological emergency in children and young adults that can lead to infertility in many cases. The ischemia-reperfusion (IR) injury due to TT has been implicated in the pathogenesis of testicular damage. The main pathological mechanisms of contralateral injury after ipsilateral TT are not fully understood. In the presented study, we investigated the molecular and microscopic basis of ipsilateral and contralateral testicular injury following ipsilateral testicular torsion detorsion (T/D) and explored the possible protective role of vitamin D3. The biochemical analysis indicated that IR injury following T/D significantly decreased the activity of testicular glutathione peroxidase (GPx) enzyme, level of serum testosterone, serum inhibin B, and expression of testicular miRNA145, while increased the activity of testicular myeloperoxidase (MPO) enzyme, level of testicular malondialdehyde (MDA), level of serum antisperm-antibody (AsAb), and expression of ADAM-17. The histological and semen analysis revealed that torsion of the testis caused damages on different tissues in testis. Interestingly, administration of vitamin D3 prior to the IR injury reversed the deterioration effect of IR injury on the testicular tissues as indicated by biochemical and histological analysis which revealed normal appearance of the seminiferous tubules with an apparent decrease in collagen fiber deposition in both ipsilateral and contralateral testes. Our results revealed that the protective effect of vitamin D3 treatment could be attributed to target miRNA145 and ADAM17 protein. To further investigate these findings, we performed a detailed molecular modelling study in order to explore the binding affinity of vitamin D3 toward ADAM17 protein. Our results revealed that vitamin D3 has the ability to bind to the active site of ADAM17 protein via a set of hydrophobic and hydrophilic interactions with high docking score. In conclusion, this study highlights the protective pharmacological application of vitamin D3 to ameliorate the damages of testicular T/D on the testicular tissues via targeting miRNA145 and ADAM17 protein.

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Testicular Torsion-Detorsion- Histological and Biochemical Changes in Rat Testis

Cited by 3 publications

References 27 publications

Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats

Effects of Coenzyme Q10 and Diamond Nanoparticles on Ischemia-Reperfusion-Induced Testicular Damages in Rats

Incidence of Low-Grade Testicular Injury in Orchidectomy Specimens Post-testicular Torsion

Vitamin D3 Prevents the Deleterious Effects of Testicular Torsion on Testis by Targeting miRNA-145 and ADAM17: In Silico and In Vivo Study

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