Testicular transcriptome analysis under the dietary inclusion of l-carnitine reveals potential key genes associated with oxidative defense and the semen quality factor in aging roosters

Elokil, Abdelmotaleb A.; Abouzaid, Mostafa; Magdy, Mahmoud; Xiao, Tengfei; Liu, H.; Xu, Renfeng; Li, S.

doi:10.1016/j.domaniend.2020.106573

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…This is particularly important since artificial insemination (AI) is utilized throughout the poultry industry. Ejaculate volume, sperm concentration, and percentage of live spermatozoa are considered core indicators of reproductive capacity in roosters ( 1 ). However, the reproductive performance of breeder animals is negatively influenced by aging, with these animals typically showing a decline in performance at ~45 weeks of age ( 2 ).…”

Section: Introductionmentioning

confidence: 99%

Dietary Lasia spinosa Thw. improves reproductive performance of aged roosters

et al. 2022

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The application of artificial insemination is particularly, owing to which breeder animals are considered an important resource in breeding farms. However, the reproductive performance of roosters typically declines with age, and the economic loss experienced by breeders is attributable to this shortened reproductive lifespan. Lasia spinosa Thw. (LST) reportedly improved reproductive capacity in male rodents. The objective of this study was to investigate the effects of LST on the reproductive performance of aged roosters. Male Guangxi Partridge chicken (mean weight, 3032.41 ± 34.48 g; age, 500 days; n = 72) randomly received the following three dietary treatments: LST0 group (a basal diet), LST2 group (a basal diet with 2% LST powder), and LST4 group (a basal diet with 4% LST powder). Computer-aided sperm analysis revealed that dietary LST supplementation significantly improved semen volume, sperm motility, and concentration. Furthermore, the most potent effects were observed in the treatment group with the administration of 2% LST, which significantly improved the weight of the testes. Hematoxylin-eosin staining revealed the increase in diameter of the seminiferous tubule and height of the seminiferous tubule epithelium possibly caused as a result of LST treatment. A significant increase in fructose and glucose concentrations were observed in the testis and seminal plasma; in addition, a significant increase was observed in the α-glycosidase levels in the testis and spermatozoa. However, the monoaldehyde levels in the spermatozoa appeared to decline significantly. Additionally, the fertility rate increased significantly following 2% LST supplementation. RNA-seq analysis revealed that 34 and 16 unigenes were upregulated and downregulated, respectively, in testicular tissues from roosters that received dietary supplementation of 2% LST. The assigned functions of the unigenes revealed that LST primarily influenced the mechanisms underlying catalytic activity and cellular processes. Kyoto Encyclopedia of Genes and Genomes enrichment analysis suggested that spermatogenesis-related pathways were significantly enriched, including ABC transporters, ribosome biogenesis in eukaryotes, and VEGF, cAMP, and ErbB signaling pathways.

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Section: Introductionmentioning

confidence: 99%

Dietary Lasia spinosa Thw. improves reproductive performance of aged roosters

et al. 2022

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“…According to the UniProt database, all the genes identified with codeml (which passed the initial test with a p-value < 0.05) are involved in general regulatory and metabolic processes, but seven were also found to be associated with various reproductive processes. According to our data, three genes associated with reproductive processes were under positive selection (with dN/dS > 1): two in L. raddei-DSG2, which is associated with spermatogonial differentiation [53], and ATP2A3, which takes part in the elaborate courtship evolution in Manakin birds, Passeriformes [54] and is also possibly associated with oxidative defense and the semen quality factor in cocks [55]; and one in L. gregalis-FBXO34, which plays a critical role in oocyte meiosis [56]. Two genes under positive selection in L. raddei were associated with different kinds of environmental adaptations-MRPS31, which is probably related to the ability to adapt to temperature gradients [57], and DSG2, which may be helpful for high-altitude adaptation [58] (Figure 4).…”

Section: Molecular Mechanisms Underlying Speciation Between Cryptic S...mentioning

confidence: 68%

Narrow-Headed Voles Species Complex (Cricetidae, Rodentia): Evidence for Species Differentiation Inferred from Transcriptome Data

et al. 2022

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The narrow-headed vole complex includes two cryptic species, Lasiopodomys raddei and L. gregalis, and three allopatrically-distributed lineages with obscure taxonomic ranks within the latter. Based on the RNA-seq data of 12 specimens, the current study aims to find the molecular mechanisms of intraspecies differentiation and, in particular, reproductive isolation between analyzed groups. According to the results of the GO-enrichment analysis, about a hundred biological processes associated with genes with contrasting SNPs for L. gregalis and L. raddei were identified. Among them, processes of interspecific interactions, defense responses, responses to external stimuli, and the perception of chemical stimuli and smell were identified, indicating the likely existence of pre-copulatory behavioral and physiological mechanisms that contribute to reproductive isolation between cryptic species. An evaluation of the ratio of non-synonymous substitutions to synonymous ones showed evidence of selection in L. raddei compared to L. gregalis for a large part of the analyzed genes. Among the analyzed genes, genes with both weakening and intensifying selection were found.

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“…The differentially expressed genes were enriched in pathways related to cytokine-cytokine receptor interactions, neuroactive ligand-receptor interactions, ECM-receptor interactions, TGF-β signaling pathway, melanogenesis, mTOR signaling pathway, Wnt signaling pathway, focal adhesion, calcium signaling pathway, and MAPK signaling pathway. The differentially expressed genes detected in chicken testis tissue samples were mainly signi cantly enriched in steroid biosynthesis, lipid metabolism and glycerol lipid metabolism [34]. Li et al found that cytokines and testosterone synergistically regulated the primary spermatocytes in sperm formation, sperm development, and the blood-testis barrier [35,36].…”

Section: Discussionmentioning

confidence: 99%

Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

liang,

he,

zhu

et al. 2024

Preprint

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Background: The breeder rooster has played an important role in poultry production by providing high-quality semen. In general, the fertility peaks of breeder roosters between 30 and 40 weeks of age and declines rapidly from 45 to 55 weeks of age. The exploration of improving fertility in aging roosters would help to extend the useful life of roosters. At present, researchers have made some progress, but improving fertility in aging roosters has become an important issue that needs to be addressed. Methods: To identify the genes related to promoting sperm remodeling in aged Houdan roosters, we combined changes in testis and semen quality with transcriptome sequencing (RNA-seq) to analyze the synchrony of semen quality and testis development. In this study, 350-day-old Houdan breeder roosters were selected for RNA-seq analysis in testis tissues from induced molting roosters (D group) and non-induced molting roosters (47DG group). All analyses of differentially expressed genes (DEGs) and functional enrichment were performed. Finally, we selected six DEGs to verify the accuracy of the sequencing by qPCR. Results: Sperm motility, sperm density, and testis weight were significantly increased in roosters in the fasted-induced molting group compared with the control group (P < 0.05). Further RNA-seq analysis of the testis between the D group and 47DG group identified 61 DEGs, with 21 up-regulated and 40 down-regulated. Functional enrichment analysis showed that the DEGs were primarily enriched in the cytokine-cytokine receptor interaction, Wnt signaling pathway, MAPK signaling pathway, TGF-β signaling pathway, and focal adhesion pathway. The qRT-PCR results showed that the expression trend of these genes was consistent with the sequencing results. WNT5A, FGFR3, AGTR2, TGFβ2, ROMO1, and SLC26A7 may play a role in testis development and spermatogenesis. This study provides fundamental data to enhance the reproductive value of aging roosters.

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Testicular transcriptome analysis under the dietary inclusion of l-carnitine reveals potential key genes associated with oxidative defense and the semen quality factor in aging roosters

Cited by 6 publications

References 29 publications

Dietary Lasia spinosa Thw. improves reproductive performance of aged roosters

Dietary Lasia spinosa Thw. improves reproductive performance of aged roosters

Narrow-Headed Voles Species Complex (Cricetidae, Rodentia): Evidence for Species Differentiation Inferred from Transcriptome Data

Dietary Restriction Promote Sperm Remodeling in Aged Roosters Based on Transcriptome Analysis

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