Testing a prototype pulse generator for a continuous flow system and its use for E. coli inactivation and microalgae lipid extraction

Flisar, Karel; Meglič, Saša Haberl; Morelj, Jernej; Golob, Janvit; Miklavčič, Damijan

doi:10.1016/j.bioelechem.2014.03.008

Cited by 84 publications

(39 citation statements)

References 62 publications

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“…In the scenarios 1, 4 and 5, the efficiency of lipids extraction (75%, 60% and 90%, respectively) was assessed. PEF extraction efficiencies between 60% and 90% have already been reported in the literature [46,49,50]. Since lipids extraction efficiency is easier to control than cell lipid content, this value was assumed to be constant (25%).…”

Section: Scenarios Descriptionmentioning

confidence: 99%

“…This extraction method does not use any toxic extraction solvent (not requiring a solvent recovery step) and is highly effective when directly applied to wet feedstocks [49,65,67]. PEF was already applied to extract lipids from C. vulgaris [46,68,69]. It was considered a clean, cheap and quick extraction process, being a promising method for the production of biodiesel and pharmaceutical and dietary products.…”

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

“…Regarding cell disruption and lipids extraction through the continuous PEF method, energy required was determined assuming the specific energy consumption reported by Flisar et al [46]. According to the authors, the energy required to process 1 L of culture broth for 1 h is 14.4 kJ, which corresponds to 4.0 kW¨m´3.…”

Section: Energy Balancementioning

confidence: 99%

“…that avoids the use of organic solvents, usually toxic, thus not affecting the biochemical composition of microalgal biomass [46,64,66]. Regarding microalgal products, PEF is considered to have high potential for the extraction of compounds, due to the low energy consumption, easy scale-up and low operational costs.…”

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

“…Microalgal biomass (S 02 -output stream of open ponds) is then harvested by flocculation in a clarifier (S 03 ) followed by centrifugation (S 04 ) to a concentration of about 20% (w/w). To avoid the use of chemicals, cell disruption procedure was the continuous pulsed electric field (PEF) tested for lipids extraction from C. vulgaris by Flisar et al [46]. The extracted lipids (S 05 ) will be sold to the biodiesel plant located nearby.…”

Section: Process Flowsheet Descriptionmentioning

confidence: 99%

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Integration of Microalgae-Based Bioenergy Production into a Petrochemical Complex: Techno-Economic Assessment

et al. 2016

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Abstract:The rapid development of modern society has resulted in an increased demand for energy, mainly from fossil fuels. The use of this source of energy has led to the accumulation of carbon dioxide (CO 2 ) in the atmosphere. In this context, microalgae culturing may be an effective solution to reduce the CO 2 concentration in the atmosphere, since these microorganisms can capture CO 2 and, simultaneously, produce bioenergy. This work consists of a techno-economic assessment of a microalgal production facility integrated in a petrochemical complex, in which established infrastructure allows efficient material and energy transport. Seven different scenarios were considered regarding photosynthetic, lipids extraction and anaerobic digestion efficiencies. This analysis has demonstrated six economically viable scenarios able to: (i) reduce CO 2 emissions from a thermoelectric power plant; (ii) treat domestic wastewaters (which were used as culture medium); and (iii) produce lipids and electrical and thermal energy. For a 100-ha facility, considering a photosynthetic efficiency of 3%, a lipids extraction efficiency of 75% and an anaerobic digestion efficiency of 45% (scenario 3), an economically viable process was obtained (net present value of 22.6 million euros), being effective in both CO 2 removal (accounting for 1.1ˆ10 4 t per year) and energy production (annual energy produced was 1.6ˆ10 7 kWh and annual lipids productivity was 1.9ˆ10 3 m 3 ).

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Section: Scenarios Descriptionmentioning

confidence: 99%

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

Section: Energy Balancementioning

confidence: 99%

Section: Cell Disruption and Lipids Extractionmentioning

confidence: 99%

Section: Process Flowsheet Descriptionmentioning

confidence: 99%

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Integration of Microalgae-Based Bioenergy Production into a Petrochemical Complex: Techno-Economic Assessment

et al. 2016

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Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Karim

Yousuf

Islam

et al. 2018

Biotechnology Progress

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The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4, and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field. The forces of the pulsing electric field caused significant damage to the cell wall of L. starkeyi and the disruption of microbial cells was visualized by field emission scanning electron microscopic (FESEM) image. After breaking the cell wall, lipid was extracted and measured to assess the efficiency of EP over other techniques. The extent of cell inactivation was up to 95% when the electrodes were placed at the distance of 2 cm, which provided high treatment intensity (36.7 kWh m ). At this condition, maximum lipid (63 mg g ) was extracted when the biomass was treated for 10 min. During the comparison, EP could extract 31.88% lipid while the amount was 11.89% for ultrasonic and 16.8% for Fenton's reagent. The results recommend that the EP is a promising technique for lowering the time and solvent usage for lipid extraction from microbial biomass. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 34:838-845, 2018.

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Microalgae as a potential source for biodiesel production: techniques, methods, and other challenges

Arenas

Palacio

Juantorena

et al. 2016

Int. J. Energy Res.

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Summary This paper reviews some of the most important aspects of microalgae as a potential source for biodiesel production. Microalgae are photosynthetic microorganisms that can grow rapidly in a variety of environments because of their unicellular or multicellular structure depending on the species. They have the advantage of self‐reproduction using solar energy and converting it into chemical energy via photosynthesis. This process concludes a full cycle in a few days, obtaining higher lipid yields than terrestrial crops. This review shows several techniques and some methodologies used in the biodiesel production process from microalgae as well as the challenges that must be overcome for large‐scale process and in bio‐refineries. Copyright © 2016 John Wiley & Sons, Ltd.

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Testing a prototype pulse generator for a continuous flow system and its use for E. coli inactivation and microalgae lipid extraction

Cited by 84 publications

References 62 publications

Integration of Microalgae-Based Bioenergy Production into a Petrochemical Complex: Techno-Economic Assessment

Integration of Microalgae-Based Bioenergy Production into a Petrochemical Complex: Techno-Economic Assessment

Microbial lipid extraction from Lipomyces starkeyi using irreversible electroporation

Microalgae as a potential source for biodiesel production: techniques, methods, and other challenges

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