Testing and validation of reciprocating positive displacement pump for benchtop pulsating flow model of cerebrospinal fluid production and other physiologic systems

Faryami, Ahmad; Menkara, Adam; Viar, Daniel; Harris, Carolyn A.

doi:10.1371/journal.pone.0262372

Cited by 8 publications

(7 citation statements)

References 35 publications

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“…The VCTD is a gravity-driven model, which is not equivalent to how CSF flows through the body. Benchtop pump systems can simulate CSF flow with varying parameters and high precision and have been proposed as modular components of in vitro hydrocephalus models [ 14 ]. Finally, the artificially obstructed catheter was obstructed using glue, which is notably different from obstruction caused by human tissue.…”

Section: Discussionmentioning

confidence: 99%

A novel, benchtop model for quantitative analysis of resistance in ventricular catheters

Gopalakrishnan,

Faryami,

Harris

2023

PLoS ONE

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Introduction The mechanisms of catheter obstruction are still poorly understood, but the literature suggests that resistance to fluid flow plays a significant role. We developed and assessed a gravity-driven device that measures flow through ventricular catheters. We used this device to quantitatively analyze the resistances of unused ventricular catheters used in the treatment of hydrocephalus; failed hydrocephalus catheters from our catheter biorepository were also evaluated quantitatively. Methods Catheters of three manufacturing companies were inserted into the benchtop model, which records time, flow rate, and pressure data using sensors. The relative resistances of catheters across six design models were evaluated. Experiments were performed to evaluate changes in the relative resistance of a catheter when the catheter’s holes were progressively closed. The relative resistance of explanted catheters from our catheter biorepository was also measured. Results Experimental results showed significant differences (P<0.05) between the relative resistances of different catheter models just after being removed from their packaging. A non-linear trend of increasing resistance was observed in experiments on catheters with artificially obstructed holes. Data from five individual benchtop models were compared, and the differences in measured data between the models were found to be negligible. A significant increase (P < 0.05) in relative resistance was observed in explanted catheters. Conclusion The current study sought to propose a novel in-vitro model and use it to examine data on differences in relative resistance among catheter models. From these experiments, we can rapidly correlate clinical patient cohorts to identify mechanisms of luminal shunt obstruction.

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Section: Discussionmentioning

confidence: 99%

A novel, benchtop model for quantitative analysis of resistance in ventricular catheters

Gopalakrishnan,

Faryami,

Harris

2023

PLoS ONE

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“…The chassis of each reciprocating positive displacement pump is comprised of 3D printed parts made of polylactic acid plastic (PLA) manufactured using an Anycubic I3 Mega printer (Anycubic Technology CO., Limited, HongKong) [ 6 ]. The design consists of five 3ml syringes per pump, allowing flow output from five total channels per pump ( Fig 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…In our previous study, the accuracy of the reciprocating positive displacement pump was demonstrated across a range of 0.01ml/min to 0.7ml/min with an R 2 value of 0.9998 [ 6 ]. The pump was originally produced in order to simulate the high shear rate pulsatile waveform of cerebral spinal fluid for in-vitro modelling scenarios.…”

Section: Introductionmentioning

confidence: 99%

Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow

et al. 2022

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Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, time, sample size, and control across current in-vitro and in-vivo methods limit future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression analysis compared to expected flow rate across the full flow range yielded an R2 of 0.9996. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow.

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“…The body of each reciprocating positive displacement pump is comprised of 3D printed parts made of polylactic acid plastic (PLA) manufactured using an Anycubic I3 Mega printer (Anycubic Technology CO., Limited, HongKong) [4]. The design consists of five 3ml syringes per pump, allowing flow output from five total channels per pump (Fig 1 .).…”

Section: Reciprocating Positive Displacement Pumpmentioning

confidence: 99%

Applications of a Novel Reciprocating Positive Displacement Pump in the Simulation of Pulsatile Arterial Blood Flow

Menkara

Faryami

Viar

et al. 2022

Preprint

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Pulsatile arterial blood flow plays an important role in vascular system mechanobiology, especially in the study of mechanisms of pathology. Limitations in cost, setup time, sample size, and control across current in-vitro and in-vivo methods prevent future exploration of novel treatments. Presented is the verification of a novel reciprocating positive displacement pump aimed at resolving these issues through the simulation of human ocular, human fingertip and skin surface, human cerebral, and rodent spleen organ systems. A range of pulsatile amplitudes, frequencies, and flow rates were simulated using pumps made of 3D printed parts incorporating a tubing system, check valve and proprietary software. Volumetric analysis of 430 total readings across a flow range of 0.025ml/min to 16ml/min determined that the pump had a mean absolute error and mean relative error of 0.041 ml/min and 1.385%, respectively. Linear regression of flow rate ranges yielded R 2 between 0.9987 and 0.9998. Waveform analysis indicated that the pump could recreate accurate beat frequency for flow ranges above 0.06ml/min at 70BPM. The verification of accurate pump output opens avenues for the development of novel long-term in-vitro benchtop models capable of looking at fluid flow scenarios previously unfeasible, including low volume-high shear rate pulsatile flow.

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Testing and validation of reciprocating positive displacement pump for benchtop pulsating flow model of cerebrospinal fluid production and other physiologic systems

Cited by 8 publications

References 35 publications

A novel, benchtop model for quantitative analysis of resistance in ventricular catheters

A novel, benchtop model for quantitative analysis of resistance in ventricular catheters

Applications of a novel reciprocating positive displacement pump in the simulation of pulsatile arterial blood flow

Applications of a Novel Reciprocating Positive Displacement Pump in the Simulation of Pulsatile Arterial Blood Flow

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