Testing for Basic Drugs in Biological Fluids by Solvent Extraction and Dual Capillary GC/NPD

Fretthold, David; Jones, Prentiss; Sebrosky, Gary; Sunshine, Irving

doi:10.1093/jat/10.1.10

Cited by 29 publications

(1 citation statement)

References 4 publications

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“…Evaluations of fused silica capillary columns for the screening of basic drugs in biological materials have been reported (210,211) and the use of dual-column fused-silica capillary GC in combination with various detectors has been described. (212)(213)(214). Wide-bore capillary columns are also gaining popularity in the use of routine toxicological screening for drugs of abuse (215)(216)(217)(218).…”

Section: Drugs and Poisonsmentioning

confidence: 99%

Forensic science

Brettell¹,

Saferstein²

1987

Anal. Chem.

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Section: Drugs and Poisonsmentioning

confidence: 99%

Forensic science

Brettell¹,

Saferstein²

1987

Anal. Chem.

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Enzyme‐amplified receptor assay screening test for chlorpromazine, trifluoperazine, and phencyclidine

Hallowell

Echnitz

1990

Clinical Laboratory Analysis

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A new receptor based assay is described for the determination of classes of drugs which have high affinities for the acetylcholine receptor. The method is based upon the inhibition of the enzyme activity of an enzyme-drug conjugate by the binding to the receptor protein, and competition between free drugs and the enzyme-drug conjugate for a limited number of receptor sites. The activity of the enzyme marker system, glucose-6-phosphate dehydrogenase covalently conjugated to desipramine, is monitored by colorimetric detection of the rate of NADH formation at 340 nM. The procedure proposed is designed to provide a simple drug screen which can be done in a minimally equipped laboratory while achieving the required sensitivity. The technique is illustrated for three acetylcholine channel binding compounds: the hallucinogen phencyclidine (PCP) and the antipsychotic agents chlorpromazine and trifluoperazine. This procedure yields calibration curves with detection limits at nanomolar levels of drug, with binding responses dependent on the amounts of receptor and enzyme-labeled drug used. Aspecific binding responses of unlabeled enzyme to drug or receptor to compounds with low affinity for the receptor are shown to have minimal effect on the assay.

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The advantage of dual‐column approach and retention indices combined with refined reporting in gas chromatographic drug screening

Rasanen

Ojanperä

Vartiovaara

et al. 1996

J. High Resol. Chromatogr.

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SummaryA dual-column gas chromatographic retention index method was evaluated for the toxicological screening for basic drugs in autopsy blood samples. The dual-column approach with DB-5 and DB-1701 capillary columns doubles the Identification Power of the corresponding single column methods. The long-term intralaboratory variation of the dialkylfluoroaniline series based retention indices of drugs in blood ranged from 0.03% to 0.2%, which was generally better than that obtained using the relative retention time. Novel software is described for the processing and reporting of the dualcolumn chromatographic data in analytically useful form. Besides retention data, the response factors served as an additional identification factor.

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Testing for Basic Drugs in Biological Fluids by Solvent Extraction and Dual Capillary GC/NPD

Cited by 29 publications

References 4 publications

Forensic science

Forensic science

Enzyme‐amplified receptor assay screening test for chlorpromazine, trifluoperazine, and phencyclidine

The advantage of dual‐column approach and retention indices combined with refined reporting in gas chromatographic drug screening

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