Testing Nanotoxicity: An Update of New and Traditional Methods

Cattaneo, Anna Giulia; Gornati, Rosalba; Bernardini, Giovanni; Sabbioni, E.; Manzo, Luigi; Gioacchino, Mario Di

doi:10.1002/9781118856017.ch1

Cited by 4 publications

(1 citation statement)

References 271 publications

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“…This potential toxicity has become a matter of concern. Some studies have reported a significant increase in inflammation markers in the airways, leakage of the blood–brain barrier, neuronal damage and cerebral oedema, after exposure to nanoparticles (Cattaneo et al, ). Zinc oxide nanoparticles show antimicrobial activity by damaging cell membranes and organelles and might cause the same kind of damage to eukaryotic cells (Marambio‐Jones & Hoek, ).…”

Section: Introductionmentioning

confidence: 99%

Comparative analysis of the toxicity of gold nanoparticles in zebrafish

Patibandla

Zhang

Tohari

et al. 2018

J of Applied Toxicology

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The use of nanoparticles - particles that range in size from 1 to 100 nm - has become increasingly prevalent in recent years, bringing with it a variety of potential toxic effects. Zebrafish embryos were exposed during the 3 day postfertilization period to gold nanospheres (GNSs), gold nanorods (GNRs), GNRs coated with polystyrene sulphate (PSS-GNRs) and GNRs coated with both PSS and polyallamine hydrochloride (PAH-PSS-GNRs). All nanorods were stabilized with cetyltrimethylammonium bromide. GNSs were the least toxic of the nanoparticles studied, with exposure resulting in no significant changes in mortality, hatching or heart rate. Exposure to GNRs and PSS-GNRs resulted in significant increases in mortality and significant decreases in hatching and heart rate. Treatment with GNRs caused significant changes in the expression of a variety of oxidative stress genes. The toxic effects of GNRs were ameliorated by coating them with PSS and, to a more marked extent, with a double coating of PSS and polyallamine hydrochloride.

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Section: Introductionmentioning

confidence: 99%

Comparative analysis of the toxicity of gold nanoparticles in zebrafish

Patibandla

Zhang

Tohari

et al. 2018

J of Applied Toxicology

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Cotreatment of Small Gold Nanoparticles Protects Against the Increase in Cerebral Acetylcholinesterase Activity and Oxidative Stress Induced by Acute Ethanol Exposure in the Zebrafish

et al. 2021

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On measuring nanoparticle toxicity and clearance with Paramecium caudatum

Mayne

Morgan

Whiting

et al. 2019

Sci Rep

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As the extent to which aquatic environments are polluted with nano-scale objects is becoming known, we are presented with an urgent need to study their effects on various forms of life and to clear and/or detoxify them. A range of methods exist to these ends, but a lack of inter-study comparability arising from an absence of experimental standardisation impedes progress. Here we present experiments that demonstrate measurement of orchestrated uptake and clearance of two environmentally-relevant nano- and micromaterials by a model aquatic microoraganism, Paramecium caudatum . Experiments were based on a simple, modular, multi-chamber platform that permits standardised control of organism behaviour and measurement of variables relevant to the study of nanotoxicology, including nanomaterial chemotaxis assays, bioaccumulation and deleterious effects on cell motility systems. Uptake of internalised materials may be estimated through the addition of a low-cost fluorescence spectrometer. P . caudatum cells can clear an estimated 0.7 fg of contaminant materials (or 161 of the particles used) per cell over a 5 mm distance per 6 hour experiment, whilst suffering few short-term adverse effects, suggesting that the organism and the platform used to investigate their properties are well-suited to a range of laboratory and field-based nanotoxicological studies.

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Testing Nanotoxicity: An Update of New and Traditional Methods

Cited by 4 publications

References 271 publications

Comparative analysis of the toxicity of gold nanoparticles in zebrafish

Comparative analysis of the toxicity of gold nanoparticles in zebrafish

Cotreatment of Small Gold Nanoparticles Protects Against the Increase in Cerebral Acetylcholinesterase Activity and Oxidative Stress Induced by Acute Ethanol Exposure in the Zebrafish

On measuring nanoparticle toxicity and clearance with Paramecium caudatum

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