The present investigation entitled ‘bio-efficacy of Novel insecticides and bio-pesticides against Diamondback moth, Plutella xylostella (Linn.) was carried out on the cabbage, variety ‘Golden Acre’ during Rabi, 2021-22 and 2022-23. At Crop Research Centre, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut (UP). The data were recorded on five randomly selected plants once in a standard week. Pooled data of both the year (2021 and 2022), After treatments, spinosad 2.50% SC at 600 ml ha-1 consistently showed the lowest larval count on days 3, 7, and 14, with 3.33, 2.17, 2.25 larvae per five plants followed by emamectin benzoate 5% SG at 200 g ha-1, diafenthiuron 50% WP at 600g ha-1, Bacillus thuringiensis var. kurstaki at 1000 g ha-1, Beauveria bassiana at 1500 ml ha-1 and Metarhizium anisopiliae at 2000 g ha-1, and nimbecidine 0.03% at 2500 ml-ha, respectively. The nimbecidine 0.03% at 2500 ml ha-1 recorded the lowest efficacy. Chemical and biological treatments varied significantly, while chemicals had similar efficacy, and biologicals were equally effective. Control plots had the highest larvae count.