Testing of chemicals for genetic activity with Saccharomyces cerevisiae: a report of the U.S. environmental protection agency gene-tox program

Zimmermann, F. K.; Borstel, R. C. von; Halle, E. Von; Parry, James M.; Siebert, D.; Zetterberg, G.; Barale, Roberto; Loprieno, N.

doi:10.1016/0165-1110(84)90017-4

Cited by 117 publications

(30 citation statements)

References 152 publications

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“…As a testing procedure, the assay according to Zimmermann et al (1984) was used. The S. cerevisiae D7 diploid tester strain (MATa/MATα, ade 2 -40/ade 2 -119, trp5-12/trp5-27, ilv1-92/ilv1-92) enables simultaneously the detection of toxicity, revertants at the ilv1 locus, mitotic crossing-over and total aberrants at the ade 2 locus, as well as mitotic gene convertants at the trp5 locus.…”

Section: Preparation Of Extract Frommentioning

confidence: 99%

Antigenotoxic effect of extract from Cynara cardunculus L.

Miadoková

Nadova

Vlčková

et al. 2007

Phytotherapy Research

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The extract of artichoke Cynara cardunculus L. (CCE) was investigated for its potential antigenotoxic and antioxidant effects using four experimental model systems. In the Saccharomyces cerevisiae mutagenicity/ antimutagenicity assay, CCE significantly reduced the frequency of 4-nitroquinoline-N-oxide-induced revertants at the ilv1 locus and mitotic gene convertants at the trp5 locus in the diploid Saccharomyces cerevisiae tester strain D7. In the simultaneous toxicity and clastogenicity/anticlastogenicity assay, it exerted an anticlastogenic effect against N-nitroso-N′ ′ ′ ′ ′-methylurea-induced clastogenicity in the plant species Vicia sativa L. On the contrary, despite CCE not being mutagenic itself, in the preincubation Ames assay with metabolic activation, it significantly increased the mutagenic effect of 2-aminofluorene in the bacterial strain Salmonella typhimurium TA98. In the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay, CCE exhibited considerable antioxidant activity. The SC 50 value representing 0.0054% CCE corresponds to an antioxidant activity of 216.8 μ μ μ μ μM ascorbic acid which was used as a reference compound. Although the mechanism of CCE action still remains to be elucidated, different possible mechanisms are probably involved in the CCE antigenotoxic effects. It could be concluded that CCE is of particular interest as a suitable candidate for an effective chemopreventive agent.

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Section: Preparation Of Extract Frommentioning

confidence: 99%

Antigenotoxic effect of extract from Cynara cardunculus L.

Miadoková

Nadova

Vlčková

et al. 2007

Phytotherapy Research

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“…Antimutagenicity test was performed using unicellular test strain S. cerevisiae D7 (ZIMMERMANN et al, 1984;MAROVA et al, 2010). The test strain was obtained from the Department of Genetics, Comenius University, Bratislava.…”

Section: Antimutagenicity Assay -The Saccharomyces Cerevisiae D7 Testmentioning

confidence: 99%

“…For genotoxicity/antimutagenicity assay eukaryotic test system S. cereviviae D7 was used. This mutant yeast strain was constructed to simultaneous testing of toxicity, reverse mutations in ilv1 locus, mitotic crossing-over and total aberrations at the ade2 locus and mitotic gene conversion at the trp5 locus (ZIMMERMANN et al, 1984;VLCKOVA et al, 2006;MIADOKOVA et al, 2010).…”

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confidence: 99%

Antimutagenic properties of several kinds of rice as tested upon yeast strainSaccharomyces cerevisiaeD7

Lichnova¹,

Márová²,

Valentova³

et al. 2014

Acta Alimentaria

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The aim of this work was to study antimutagenic and antioxidant properties of water extracts of various types of rice. Antioxidant activity, total phenolic and fl avonoid contents were measured spectrophotometrically. Individual phenolics -catechins, catechin gallates, ferulic acid -were analysed by HPLC/PDA. Antimutagenic activity of rice extracts was tested using unicellular eukaryotic yeast strain Saccharomyces cerevisiae D7 the fi rst time.Non-processed rice types contained substantially higher amount of soluble total and individual phenolics and had higher antioxidant activity than peeled rice. Parboiled rice, in which technological processing helps to maintain active substances inside the grains, exhibited also relatively high phenolic levels. The highest antimutagenic effect was proved in Indian Rice, Arborio, Jasmine Rice and Three Colours Rice independently of rice extract concentration. Because of relatively high values of phenolics and high antioxidant activity, the antimutagenic activity of these samples could be attributed to biologically active phenolics present mainly in the bran layer of whole rice grain.

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“…Furthermore, similar situation were observed in Escherichia coli and Bacillus subtilis when the reverse mutation assay was applied (Simmon, 1979;Leifer et al, 1981;Waters et al, 1981). Whereas the herbicide was unable to induce mitotic recombination in Saccharomyces cerevisiae (Zimmermann et al, 1984), negative and positive results were obtained for the induction of unscheduled DNA synthesis in human primary cells regardless of the presence or absence of a rat liver metabolic activation system (Simmon, 1979;Perocco et al, 1990). Induction of DNA single-strand breaks, estimated by the alkaline comet assay, was evaluated in CHO cells exposed in vitro to Dicamba.…”

Section: Genotoxicity and Cytotoxicity Of Dicambamentioning

confidence: 99%