Testing patients with uveal melanoma for herpesvirus infections

Abstract

Results of comprehensive ELISA tests of blood serum for the presence of IgM-, IgA-, and IgG-antibodies to herpes simplex virus types 1 and 2, cytomegalovirus, Epstein-Barr virus, human herpesvirus 6, human herpes virus 8 type, Chlamydia trachomatis in 38 patients with uveal melanoma are presented. The polymerase chain reaction was used to detect DNA of these pathogens in tumor biopsies, vitreous body of 10 enucleated eyes, as well as in plasma IgG-antibodies to HHV 6 were revealed in 50% of patients; IgG-antibodies to HHV 8, in 5.3% of patients. Among the 16 patients with uveal melanoma at advanced stages, 6 patients had antibodies indicative of EBV reactivation (1.2-3.3). Chlamydia trachomatis genome was detected in both biopsies; in one of them, in conjunction with EBV and CMV DNA . Tissue samples from the identified infectious agents were related only to the spindle-cell histologic type AB of uveal melanoma. In plasma, genomes of pathogens were not determined. The results indicate the presence of infectious agents in patients with uveal melanoma and require further study of the pathogenetic role of infections in the pathogenesis of uveal melanoma.

Quorum Sensing System in Bacteroides thetaiotaomicron Strain Identified by Genome Sequence Analysis

“…The literary sources provide a wide spectrum of data regarding the microbiome of the human gastrointestinal tract. 7 There have been reports on the variation in metagenome composition of microorganisms linked to a range of factors. 10 In ulcerative colitis, an elevated level of Bacteroides and Prevotella spp.…”

“…), blocking the action of autoinductors and inhibiting the action of QS signaling molecules. 7 The widely studied QSI are antagonistic peptides from natural resources, in particular polyphenols that can be found in tea, honey, garlic, cloves, marine organisms, and fungi. The synthetic QS-inhibiting substances include the preparations of 5-fluorouracil, azithromycin, poly(ethylene glycol), furanone derivatives, thiazolidinone derivatives, etc.…”

“…The disclosure of the functions of the multiple ECF σ-factors present in many genomes poses a huge challenge for future research. 7 , 8 …”

Features of folate cycle disorders in children with ASD

“…Violations were noted in the humoral immunity.43% of children were diagnosed with dysimmunoglobulinemia with immunoglobulins subclass deficiency, either isolated or combined, but it was most often shallow and transient. Based on immune status analysis, the main immunological phenotypes in children with a folate cycle disorders can be distinguished 15 . The main immunological phenotype was NK and / or NKT cells deficiency.…”

“…Polymorphisms of folate cycle enzymes are considered as a significant reason for thegenetic locationto the development of autistic disorders 6,7 . In addition, polymorphisms of genes involved in thefolic acid cycle are associated with hyperhomocysteinemia, cardiovascular catastrophes 8 and the later stage of dementia 9 , congenital anomalies, primarily the nervous system 5,10 , and pathology in pregnancy, including multiple spontaneous abortions 11 , a high risk of cancer developing, including colon and rectal 12 , due to a DNA methylation [13][14][15] . There are few reports of autism in adults and children suffered from Neuroinfectious diseases, mainly the opportunistic infections 10,16 .…”