Nitrogen limits crop production when insufficient and harms the environment when excessive. Tailoring N inputs to cropping systems remains a high priority to achieve production and environmental goals. We collected soils from 47 corn (Zea mays L.) production fields in North Carolina and Virginia at depths of 0 to 10, 10 to 20, and 20 to 30 cm and evaluated soil C and N characteristics in association with soil N mineralization. Soil organic C at a depth of 0 to 10 cm varied among sites from ~10 to 80 g kg -1 , and generally declined with depth because of many sites with no-tillage management. N itrogen (N) is considered the most limiting nutrient in plant production. In many cases, demand for N by highly productive crops far exceeds the N that can be supplied by soil. Sources of N from soil are residual inorganic N from previous cropping or organic forms of N in soil organic matter and plant and animal residues, which must be mineralized in synchrony with decomposition processes. Insufficient N leads to low biomass production as a result of limited protein synthesis and low photosynthetic activity, all of which causes cascading negative effects on water-use efficiency, biological activity, ecosystem functioning, economic return from farm capital investment, and social welfare of farming communities (Smil, 2002;Tilman et al., 2011). In contrast, excessive N leads to susceptibility of crops to invasion by pests, leakage of N from the soil and plant systems to the environment causing air and water pollution, and loss of investment from costly inorganic N inputs (Vitousek et al., 1997;Hatfield and Follett, 2008).Nitrogen availability in soils has been investigated for decades (Waksman and Starkey, 1924;Fribourg and Bartholomew, 1956;Stanford, 1968;Jenkinson and Powlson, 1976;Jansson and Persson, 1982;Campbell et al., 1991), yet reliable predictions of N fertilizer application rate to optimize cereal grain yields have been elusive (Balkcom et al., 2003). Early investigations to optimize inorganic N inputs focused on defining potentially mineralizable N from a nonlinear function derived from inorganic N released through successive leaching and incubation (Stanford and Smith, 1972). This methodology has been considered the best estimate of soil N mineralization potential, despite (i) the long time period for evaluation (32 wk
Core Ideas• Soil nitrogen mineralization can be predicted with the flush of CO 2 .• Soil texture does not alter the relationship between the flush of CO 2 and N mineralization.• Large quantity of mineralizable N in surface soils is possible with conservation management.• The flush of CO 2 is an appropriate indicator for soil-test biological activity.• The flush of CO 2 is a rapid and reliable indicator of soil N availability.