Testing the Potential of Proposed DNA Barcoding Markers in<i>Nezara virudula</i>and<i>Nezara antennata</i>When Geographic Variation and Closely Related Species Were Considered

Li, Min; Liu, Qiang; Li, Xi; Liu, Yang; Zhu, Gengping; Zhao, Yanni; Bu, Wenjun

doi:10.1673/031.014.79

Cited by 5 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, population #4‐ Sterculia clustered with the distant #5‐ Sterculia and #6‐ Adansonia populations despite being closer to several Welwitschia populations; additionally, populations #1/11‐ Welwitschia did not cluster with the relatively close #12/13‐ Sterculia populations, instead appearing more closely related to #2/9‐ Welwitschia populations that are a similar distance (#2) or even further away (#9) (Figure ). This provides some evidence suggesting genetic differentiation based on host plant use and the potential presence of host‐specialized races (Dres & Mallet, ), although the >99.6% similarity of the CO1 gene across all individuals measured is well within the 3% and 5% interspecies thresholds proposed previously (Hebert, Cywinska, & Ball, ; Kaur & Sharma, ; Li et al, ), indicating that all are members of the same species.…”

Section: Resultssupporting

confidence: 75%

“…#12/13-Sterculia populations, instead appearing more closely related to #2/9-Welwitschia populations that are a similar distance (#2) or even further away (#9) ( Figure S2). This provides some evidence suggesting genetic differentiation based on host plant use and the potential presence of host-specialized races (Dres & Mallet, 2002), although the >99.6% similarity of the CO1 gene across all individuals measured is well within the 3% and 5% interspecies thresholds proposed previously (Hebert, Cywinska, & Ball, 2003;Kaur & Sharma, 2017;Li et al, 2014), indicating that all are members of the same species. (Table 2).…”

Section: Probergrothius Populations Feeding On Welwitschia and Malvsupporting

confidence: 70%

See 1 more Smart Citation

Angiosperm to Gymnosperm host‐plant switch entails shifts in microbiota of theWelwitschiabug,Probergrothius angolensis(Distant, 1902)

et al. 2019

View full text Add to dashboard Cite

The adaptation of herbivorous insects to new host plants is key to their evolutionary success in diverse environments. Many insects are associated with mutualistic gut bacteria that contribute to the host's nutrition and can thereby facilitate dietary switching in polyphagous insects. However, how gut microbial communities differ between populations of the same species that feed on different host plants remains poorly understood. Most species of Pyrrhocoridae (Hemiptera: Heteroptera) are specialist seed‐feeders on plants in the family Malvaceae, although populations of one species, Probergrothius angolensis, have switched to the very distantly related Welwitschia mirabilis plant in the Namib Desert. We first compared the development and survival of laboratory populations of Pr. angolensis with two other pyrrhocorids on seeds of Welwitschia and found only Pr. angolensis was capable of successfully completing its development. We then collected Pr. angolensis in Namibia from Malvaceae and Welwitschia host plants, respectively, to assess their bacterial and fungal community profiles using high‐throughput amplicon sequencing. Comparison with long‐term laboratory‐reared insects indicated stable associations of Pr. angolensis with core bacteria (Commensalibacter, Enterococcus, Bartonella and Klebsiella), but not with fungi or yeasts. Phylogenetic analyses of core bacteria revealed relationships to other insect‐associated bacteria, but also found new taxa indicating potential host‐specialized nutritional roles. Importantly, the microbial community profiles of bugs feeding on Welwitschia versus Malvaceae revealed stark and consistent differences in the relative abundance of core bacterial taxa that correlate with the host‐plant switch; we were able to reproduce this result through feeding experiments. Thus, a dynamic gut microbiota may provide a means for insect adaptation to new host plants in new environments when food plants are extremely divergent.

show abstract

Section: Resultssupporting

confidence: 75%

Section: Probergrothius Populations Feeding On Welwitschia and Malvsupporting

confidence: 70%

Angiosperm to Gymnosperm host‐plant switch entails shifts in microbiota of theWelwitschiabug,Probergrothius angolensis(Distant, 1902)

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Genetic diversity of Pentatomidae species has mostly been studied using the COI gene region [50-52, 54, 55], although additional genes encoded by the mitochondria such as the cytochrome b (Cytb) are also commonly used [49,56]. The occurrence and genetic diversity of Halyomorpha halys (Stål) has been widely studied in several countries [57][58][59][60][61][62] and such information is also available for N. viridula [63][64][65]. For example, in Brazil, various studies examined population structure and variation among populations of N. viridula [66,67], E. heros [68,69] and Loxa spp.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the two-spotted stink bug Bathycoelia distincta (Pentatomidae) associated with macadamia orchards in South Africa

Pal

Allison²,

Hurley³

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

The South African macadamia industry is severely affected by a complex of stink bugs, dominated by the two-spotted stink bug, Bathycoelia distincta Distant (Pentatomidae). This species was first discovered during the spring of 1984 in the Limpopo province. Although considerable effort has been spent trying to manage this pest, it continues to be a pest of concern for the macadamia industry. Information on the genetic diversity of this species is lacking, despite the potential relevance of such information for management strategies. The present study aimed to characterise the genetic diversity of B. distincta populations in South Africa. The Cytochrome c Oxidase Subunit 1 (COI) and cytochrome b (Cytb) gene regions were sequenced from individuals collected from the three main regions of macadamia production over three different seasons (2018–2020). An overall high haplotype diversity (COI = 0.744, Cytb = 0.549 and COI+Cytb = 0.875) was observed. Pairwise mean genetic distance between populations from each region varied from 0.2–0.4% in both datasets, which suggests the absence of cryptic species. The median joining network for both datasets consisted of one or two central haplotypes shared between the regions in addition to unique haplotypes observed in each region. Finally, low genetic differentiation (FST < 0.1), high gene flow (Nm > 1) and the absence of a correlation between genetic and geographic distance were estimated among populations. Overall, these results suggest that the B. distincta populations are not structured among the areas of macadamia production in South Africa. This might be due to its ability to feed and reproduce on various plants and its high dispersal (airborne) between the different growing regions of the country along with the rapid expansion of macadamia plantations in South Africa.

show abstract

“…The specimen from Botswana may represent a misidentified individual of another Nezara species rather than a new species within what is currently described as N. viridula, but N. viridula is widely reported from Africa (Afreh-Nuamah 1983; Ivbijaro & Bolaji 1990;Khaemba & Mutinga 1982;Poutouli 1995;Sithole et al 1986). The conclusion that the African lineage represents a distinct species was also reached by Li et al (2014) based on the same evidence, but more than the single sample from Botswana will be required from across Africa to test the relationship of African N. viridula to those found elsewhere.…”

Section: The Biogeographical History Of the Genus Nezaramentioning

confidence: 83%

The interaction between diet breadth, geography and gene flow in herbivorous insects

Brookes¹

View full text Add to dashboard Cite

The geographical distribution and life history of insects and their host plants determines when, and if, they will interact. The vast majority of herbivorous insects are host-plant specialists and their narrow host range limits their distribution and may also restrict gene flow between their populations. Generalist insect herbivores, by contrast, might be expected to have much higher gene flow across populations because the distribution of multiple host plant species is likely to be much more contiguous. Species distributions shift continually, so with enough time the populations of a specialist insect species should experience more events that fracture their distribution because of the changing distribution of their relatively fewer hosts. The changing distribution of insects and their host plants is thus important for mediating host-plant interactions and will also influence how and when adaptation to a host plant, or subset of host plants, occurs.To investigate the influence of geography and host breadth on gene flow, and thus speciation, I explore the evolutionary history and population genetic structure of two herbivorous insects with different host plant relationships, the generalist bug Nezara viridula (Pentatomidae, Hemiptera), a global pest species, and the specialist thrips Cycadothrips chadwicki (Aeolothripidae, Thysanoptera), the pollinator in a brood-site mutualism with Macrozamia cycads. The results from these systems were then integrated with other similar data sets from phytophagous insects to understand better how gene flow and host specificity relate to one another, and to examine the role each has played (and may still play) in the evolution of insect-plant interactions more generally.The pest bug N. viridula has two globally distributed mitochondrial lineages, the Asian (mtDNA lineage A) and the European (mtDNA lineage E), and the species status of these lineages has not been resolved. In Australia, I found that both of these N. viridula mitochondrial lineages were present.Microsatellite data show two genetically and geographically distinct populations, but these did not correspond directly to the two mitochondrial lineages. Together, mtDNA and microsatellite data shown that secondary contact has occurred in eastern Australia between the two lineages and has resulted in introgression of mtDNA lineage A into predominantly mtDNA lineage E populations in a narrow area, but without widespread introgression across the nuclear genome. Past research on this insect suggested that these lineages represented a single species because these mtDNA haplotypes were found together in single populations. The evidence presented here indicates that mating between lineages can occur, but that an analysis of mtDNA alone is not enough to understand the evolutionary history of these Conference abstracts for both of the above references can be found in Appendix 2. Publications included in thesis Contributor Statement of contribution Dean BrookesDesigned the study (50%), developed molecular tools (60%), cond...

show abstract

Testing the Potential of Proposed DNA Barcoding Markers inNezara virudulaandNezara antennataWhen Geographic Variation and Closely Related Species Were Considered

Cited by 5 publications

References 37 publications

Angiosperm to Gymnosperm host‐plant switch entails shifts in microbiota of theWelwitschiabug,Probergrothius angolensis(Distant, 1902)

Angiosperm to Gymnosperm host‐plant switch entails shifts in microbiota of theWelwitschiabug,Probergrothius angolensis(Distant, 1902)

Genetic diversity of the two-spotted stink bug Bathycoelia distincta (Pentatomidae) associated with macadamia orchards in South Africa

The interaction between diet breadth, geography and gene flow in herbivorous insects

Contact Info

Product

Resources

About