Testing the role of the cell-surface molecule Thy-1 in regeneration and plasticity of connectivity in the CNS

Barlow, Jennifer Z.; Kelley, Kevin; Bozdagi, Ozlem; Huntley, George W.

doi:10.1016/s0306-4522(02)00023-4

Cited by 22 publications

(19 citation statements)

References 88 publications

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“…The presence of multiple cell adhesion molecule-like domains in MDGA1 leads to speculation a functional role related to cellular adhesion for this protein. The immunoglobulin superfamily, the largest class of cellular adhesion molecules (CAMs), includes some well characterized proteins with structural features similar to MDGA1, containing a variable number of Ig-like motifs in their extracellular domain and a GPI anchor to the cell membrane [5][6][7][8][9]. Regarding MAM domain, also present in MDGA1, this domain is thought to have an adhesive function, being present among several CAMs implicated in cell to cell interaction [10,11].…”

Section: Introductionmentioning

confidence: 99%

Characterization of MDGA1, a novel human glycosylphosphatidylinositol-anchored protein localized in lipid rafts

Díaz‐López

Rivas

Iniesta

et al. 2005

Experimental Cell Research

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Section: Introductionmentioning

confidence: 99%

Characterization of MDGA1, a novel human glycosylphosphatidylinositol-anchored protein localized in lipid rafts

Díaz‐López

Rivas

Iniesta

et al. 2005

Experimental Cell Research

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“…Thy-1 is also expressed in certain stages of lymphocytes, fibroblasts, endothelial cells, and glial cells; however, tissue distributions of Thy-1 differ among different animal species [Henniker, 2001;Barlow et al, 2002]. Thy-1 belongs to the immunoglobulin superfamily, which is a group of cell surface glycoproteins sharing structural motifs related to immunoglobulin and fibronectin domains [Walsh and Doherty, 1992].…”

mentioning

confidence: 99%

“…Thy-1 null mutant mice appear normal in neural development and neuronal architecture [Nosten-Bertrand et al, 1996;Barlow et al, 2002]. In these Thy-1 null mutant mice, however, there is a specific deficit in long-term potentiation in dentate gyrus of hippocampus [Nosten-Bertrand et al, 1996].…”

mentioning

confidence: 99%

Role of Thy‐1 in in vivo and in vitro neural development and regeneration of dorsal root ganglionic neurons

Chen

Wang

Jeng

2004

J of Cellular Biochemistry

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We have examined the expression of Thy-1, an abundant glycosylphosphatidylinositol (GPI)-anchored glycoprotein, in dorsal root ganglia (DRG) and associated nerve fascicles, during postnatal development and following a nerve crush. The expression levels of Thy-1 in DRG neurons, dorsal roots, and central processes in spinal cord were rather low at postnatal day 2, and gradually increased as DRG neurons matured. During early development, the expression of Thy-1 within DRG neurons was low and equally distributed between plasma membrane and cytosol. With maturation, the staining intensities of Thy-1 in both the plasma membrane and the cytosol of DRG neurons became increased. We also studied Thy-1 expression in the regeneration of mature DRG neurons following the crush injury of sciatic nerve. Two days after the crush injury, Thy-1 expression dramatically decreased in the DRG neurons on the lesion side. Between 4 and 7 days after the injury, the expression of Thy-1 gradually increased and returned to a normal level 1 week after the sciatic nerve crush. The time course of the up-regulation of Thy-1 expression during regeneration matched that of the recovery of sensory functions, such as pain withdraw reflex, placing reflex, and the score of Basso-Beattie-Bresnahan Locomotor Rating Scale. Taken together, our results suggest that Thy-1 expression is developmentally regulated and is closely associated with the functional maturation of DRG neurons during both postnatal development and nerve regeneration. Furthermore, perturbation of Thy-1 function with anti-Thy-1 antibodies promoted neurite outgrowth from primary cultured DRG neurons, again confirming the inhibitory role of Thy-1 on neurite outgrowth.

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“…Thy-1 has been implicated in T cell receptor-mediated and lipid raft based signal transduction. 7,8 Thy-1 was also suggested to play a role in cell adhesion 9,10 and inhibition of neurite outgrowth, 11 but Thy-1 knockout mice did not show an increased capacity of regeneration 12 or other apparent defects. The expression of Thy-1 is developmentally regulated.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary Analysis and Expression of Teleost Thy-1

et al. 2004

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Thy-1 is a developmentally regulated, immunoglobulin superfamily member (IgSF), glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein expressed most strongly in neurons and lymphocytes. Thy-1 is expressed in all vertebrates and has been implicated in a variety of processes, including axon regeneration and transmembrane signaling, but its specific function remains elusive. A Thy-1-like molecule in teleost fish was recently identified, with evidence for its role in lipid-raft based signal transduction linked to optic nerve regeneration. For a better characterization of Thy-1, the evolutionary relationships between novel fish homologues and other vertebrate Thy-1s were analyzed. Although the sequence similarity between fish and mammals is very low, there appeared conservation of gene structure and disrupted but recognizable synteny. In addition, the detailed expression analysis of teleost Thy-1 showed nervous system Thy-1 mainly in sensory systems. Strong Thy-1 expression was detected in the youngest retinal ganglion cells and in some neurons in deeper retinal layers, probably amacrine cells. From the olfactory bulbs, Thy-1-positive cells extended axons into the telencephalon. The vagal lobe stained intensively as well as facial and glossopharyngeal lobes and nerves. Outside the CNS, skin cells, blood vessels, kidney macrophages, swim bladder, spleen, gut-associated nerve fibers and the palatal organ were labeled.

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Testing the role of the cell-surface molecule Thy-1 in regeneration and plasticity of connectivity in the CNS

Cited by 22 publications

References 88 publications

Characterization of MDGA1, a novel human glycosylphosphatidylinositol-anchored protein localized in lipid rafts

Characterization of MDGA1, a novel human glycosylphosphatidylinositol-anchored protein localized in lipid rafts

Role of Thy‐1 in in vivo and in vitro neural development and regeneration of dorsal root ganglionic neurons

Evolutionary Analysis and Expression of Teleost Thy-1

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