Testing the Waste Based Biorefinery Concept: Pilot Scale Cultivation of Microalgal Species on Spent Anaerobic Digestate Fluids

Silkina, Alla; Zacharof, Myrto-Panagiota; Ginnever, Naomi E.; Gerardo, Michael L.; Lovitt, Robert W.

doi:10.1007/s12649-019-00766-y

Cited by 5 publications

(3 citation statements)

References 49 publications

(90 reference statements)

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“…Consequently, the majority of AD plants are under pressure to find alternative solutions for their excess digestate, which is currently stored or buried. Microalgae have been widely studied to remediate wastewater from water treatment processes, aquaculture facilities, and other industries [3][4][5], and there has been a growing interest in utilising their bioremediation properties to tackle the growing digestate issue in Northwest Europe [6][7][8]. Indeed, digestate composition shows a vast potential to support microalgal growth, especially in terms of macronutrients such as phosphorus and nitrogen [9,10], and also other microelements, which, at the right concentrations, can provide essential nutrition to microalgae [11].…”

Section: Introductionmentioning

confidence: 99%

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

et al. 2022

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The bioremediation of digestate using microalgae presents a solution to the current eutrophication issue in Northwest Europe, where the use of digestate as soil fertiliser is limited, thus resulting in an excess of digestate. Ammonium is the main nutrient of interest in digestate for microalgal cultivation, and improving its availability and consequent uptake is crucial for optimal bioremediation. This work aimed to determine the influence of pH on ammonium availability in cultures of two green microalgae, additionally screened for their growth performances on three digestates produced from different feedstocks, demonstrating the importance of tailoring a microalgal strain and digestate for bioremediation purposes. Results showed that an acidic pH of 6–6.5 resulted in a better ammonium availability in the digestate media, translated into better growth yields for both S. obliquus (GR: 0.099 ± 0.001 day−1; DW: 0.23 ± 0.02 g L−1) and C. vulgaris (GR: 0.09 ± 0.001 day−1; DW: 0.49 ± 0.012 g L−1). This result was especially true when considering larger-scale applications where ammonium loss via evaporation should be avoided. The results also demonstrated that digestates from different feedstocks resulted in different growth yields and biomass composition, especially fatty acids, for which, a digestate produced from pig manure resulted in acid contents of 6.94 ± 0.033% DW and 4.91 ± 0.3% DW in S. obliquus and C. vulgaris, respectively. Finally, this work demonstrated that the acclimation of microalgae to novel nutrient sources should be carefully considered, as it could convey significant advantages in terms of biomass composition, especially fatty acids and carbohydrate, for which, this study also demonstrated the importance of harvesting time.

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Section: Introductionmentioning

confidence: 99%

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

et al. 2022

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“…Therefore, there is a real need to seek, develop, and optimize methods that could serve as a competitive alternative to the current solutions. One of the most promising and encouraging options is found in the development of technologies that use waste substrates as the main growth medium ingredient [7,8], a method consistent with the idea of the circular economy and the principles of an integrated biorefinery approach [9]. With the use of biorefinery complexity index (BCI) as an indicator of technical and economic risk, algal-and waste-based bio-refinery platforms are considered to be one of the most promising approaches for producing fuel, food, animal feed, food supplements, fertilizers, and pharmaceuticals [10].…”

Section: Introductionmentioning

confidence: 99%

Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol

Kujawska¹,

Talbierz²,

Dębowski

et al. 2021

Energies

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The aim of this study was to optimize biomass and docosahexaenoic acid (DHA) production by Schizochytrium sp. grown on waste glycerol as an organic carbon source. Parameters having a significant effect on biomass and DHA yields were screened using the fractional Plackett–Burman design and the response surface methodology (RSM). Schizochytrium sp. growth was most significantly influenced by crude glycerin concentration in the growth medium (150 g/dm3), process temperature (27 °C), oxygen in the bioreactor (49.99% v/v), and the concentration of peptone as a source of nitrogen (9.99 g/dm3). The process parameter values identified as optimal for producing high DHA concentrations in the biomass were as follows: glycerin concentration 149.99 g/dm3, temperature 26 °C, oxygen concentration 30% (v/v), and peptone concentration 2.21 g/dm3. The dry cell weight (DCW) obtained under actual laboratory conditions was 66.69 ± 0.66 g/dm3, i.e., 1.27% lower than the predicted value. The DHA concentration obtained in the actual culture was at 17.25 ± 0.33 g/dm3, which was 3.03% lower than the predicted value. The results obtained suggest that a two-step culture system should be employed, with the first phase focused on high production of Schizochytrium sp. biomass, and the second focused on increasing DHA concentration in the cells.

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“…However, their production also creates additional quantities of CO 2 and wastewaters. Therefore, scientists are proposing biofuels facilities with less CO 2 emission and cleaner wastewater [1,2].…”

Section: Introductionmentioning

confidence: 99%

Cultivation of Chlamydomonas reinhardtii in Anaerobically Digested Vinasse for Bioethanol Production

Tasić

Bonon

Schiavon

et al. 2020

Waste Biomass Valor

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This study describes a new algal biofuel process that integrates sugarcane biorefinery wastewater treatment by nutrient removal with algae into bioethanol. The process is free of common industrial problems, including algal contamination, nutrients and fresh water usage, carbohydrate extraction, liquefaction, and saccharification. Cultivation and fermentation were conducted in one step by turning the light-air on and off, respectively. Three series of experiments with Chlamydomonas reinhardtii CC-1093 cultivation and fermentation were performed in anaerobically digested vinasse. Control experiments were a reference to compare the influence of chloride and ammonium-sulfate stress conditions on ethanol yield. Experimental results showed: (1) algal biomass can be successfully cultured within biorefinery wastewater (1129 mg•L −1 •day −1 );(2) relatively high bioremediation was achieved (26.1%-83.5%); (3) obtained ethanol yield was (maximum 68.3% of the theoretical yield) in one process step; and (4) the chloride stress condition influences on algae to synthesize extracellular polysaccharides as add-in product (120 mg/L).

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Testing the Waste Based Biorefinery Concept: Pilot Scale Cultivation of Microalgal Species on Spent Anaerobic Digestate Fluids

Cited by 5 publications

References 49 publications

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Microalgae Cultivation on Nutrient Rich Digestate: The Importance of Strain and Digestate Tailoring under PH Control

Optimizing Docosahexaenoic Acid (DHA) Production by Schizochytrium sp. Grown on Waste Glycerol

Cultivation of Chlamydomonas reinhardtii in Anaerobically Digested Vinasse for Bioethanol Production

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