Testis Development, Sperm Quality Evaluation and Cryopreservation in the European Eel

Pérez, Luz; Peñaranda, David S.; Gallego, Víctor; Asturiano, Juan F.

doi:10.1007/978-1-4020-9095-0_14

Cited by 11 publications

(21 citation statements)

References 97 publications

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“…No coverslip was added for sperm activation. Motility was assessed at 400× magnification, within 10 s after activation, using an arbitrary scale where 0 represents no motile sperm, I 25%, II 25%–50%, III 50%–75%, IV 75%–90% and V 90%–100% motile sperm (Pérez, Peñaranda, Gallego, & Asturiano, ). All samples were performed in triplicate and analysed by the same trained observer.…”

Section: Methodsmentioning

confidence: 99%

Effect of parental origin on early life history traits of European eel

Benini¹,

Politis

Kottmann

et al. 2018

Reprod Domestic Animals

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Establishment of European eel (Anguilla anguilla) hatchery production will rely on selectively bred individuals that produce progeny with the best traits in successive generations. As such, this study used a quantitative genetic breeding design, between four females and nine males (four wild-caught and five cultured), to investigate the effect of paternal origin (wild-caught vs. cultured) and quantify the relative importance of parental effects, including genetic compatibility, on early life history (ELH) performance traits (i.e. fertilization success, embryonic survival at 32 hr post-fertilization, hatch success and larval deformities at 2 days post-hatch) of European eel. Wild-caught males had higher (56%) spermatocrit values than cultured males (45%), while fertilization success, embryonic survival, hatch success and larval deformities were not significantly impacted by paternal origin. This demonstrates that short-term domestication of male eels does not negatively affect offspring quality and enables the consideration of cultured male broodstock in future breeding programmes. Moreover, paternity significantly explained 9.5% of the variability in embryonic survival, providing further evidence that paternal effects need to be taken into consideration in assisted reproduction protocols. Furthermore, maternity significantly explained 54.8% of the variation for fertilization success, 61.7% for embryonic survival, 88.1% for hatching success and 62.8% for larval deformities, validating that maternity is a major factor influencing these "critical" ELH traits. At last, the parental interaction explained 12.8% of the variation for fertilization success, 8.3% for embryonic survival, 4.5% for hatch success and 20.5% for larval deformities. Thus, we conclude that eggs of one female can develop more successfully when crossed with a compatible male, highlighting the importance of mate choice for successful propagation of high-quality offspring. Together, this knowledge will improve early offspring performance, leading to future breeding programmes for this critically endangered and economically important species.

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Section: Methodsmentioning

confidence: 99%

Effect of parental origin on early life history traits of European eel

Benini¹,

Politis

Kottmann

et al. 2018

Reprod Domestic Animals

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“…Motility of each replicate was characterized to the nearest 10% increment, averaged and then categorized into an arbitrary scale where 0: represents no motile sperm; while I: <25%; II: 25–50%; III: 50–75%; IV: 75–90%; and V: 90–100% represent per cent of motile spermatozoa (Pérez et al. ).…”

Section: Methodsmentioning

confidence: 99%

“…). After the 8th week, only minor changes in spermatozoa/sperm cells head size occur, followed by a decrease in head length from the 12th week and onwards (Marco Jiménez et al 2006; Pérez et al ; Peñaranda et al. ).…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Methods to Determine Sperm Density for the European eel, Anguilla anguilla

Sørensen

Gallego

Pérez

et al. 2013

Reprod Domestic Animals

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WileySorensen, S.; Gallego Albiach, V.; Pérez Igualada, LM.; Butts, I.; Tomkiewicz, J.; Asturiano Nemesio, JF. (2013). Evaluation of methods to determine sperm density for the European eel, Anguilla anguilla. Reproduction in Domestic Animals. 48 (6) flow-cytometry (FCM), using Neubauer Improved hemocytometer as benchmark. 27Initially, relationships between spermatocrit, hemocytometer counts, and sperm motility 28 were analyzed, as well as the effect of sperm dilution on hemocytometer counts. 29Furthermore, accuracy and precision of spermatocrit, applying a range of G-forces, were 30 tested and the best G-force used in method comparisons. 31We found no effect of dilution on hemocytometer sperm density estimates, whereas 32 motility associated positively with hemocytometer counts, but not with spermatocrit. 33Results from all techniques, spermatocrit, CASA and FCM, showed significant positive Results 289Trial 1: Relationships between spermatocrit, sperm density, and motility 290Spermatocrit at 12,000 × g ranged from 12.3 to 100% and hemocytometer counts 291 ranged from 1.4 to 21.4 × 10 9 sperm mL -1 (Fig. 1). For these 43 males, there was a The CVs for CASA-1 (17.9%) and CASA-2 (28.4%) were in the order of 7.5 times 338 greater compared to the other counting techniques (CV ranges from 2.5 to 5.9%; Table 339 1). before sperm density was quantified using a hemocytometer. This study by Rakitin et al. 372(1999) used a one-step 500-fold dilution and their reported variability was high (CV =

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“…In the case of the European eel, the temperature of the hypothetical spawning area is around 20 ºC (Boëtius and Boëtius, 1967), and that is the reason why the maturation of males and females of this species has traditionally been performed in water of that temperature (Peñaranda et al, 2010;Pérez et al, 2009). However, the influence of temperature on the maturation process of the European eel has recently been noted both in females Pérez et al, 2011) and in males (Baeza et al, 2015;Tanaka et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Molecular approaches related to the European eel (Anguilla anguilla) reproductive process

Morini¹

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ACKNOWLEDGEMENTSEn primer lugar quiero dar las gracias a Johnny por haberme dado la oportunidad de realizar este doctorado. Muchas gracias por la ayuda y los buenos consejos prestados. Agradezco mucho tu comprensión y empatía cada vez que he estado malita.Gracias también a Luz, por tu buen humor y tu gran simpatía. Me has hecho sentir muy cómoda desde el principio y además me has convertido en fanática gatuna.David, muchas gracias por haber confiado en mi desde el principio, darme libertad en la realización de los experimentos y haber resuelto todas las dudas que iban surgiendo.Por otra parte quería dedicar un párrafo muy especial a mis primeros amigos españoles, mis preferidos y que a la vez eran mis compañeros de trabajo.Como podéis imaginar siempre es difícil llegar a otro país, lejos de la familia, de los amigos de la infancia y sin hablar el idioma. Vosotros me lo habéis hecho todo más fácil. He aprendido mucho a vuestro lado y he evolucionado mucho gracias a vosotros, de ser sepia (gracias Vic) a casi valenciana con acento francés. Me lo he pasado fenomenal entre las cervecitas del viernes, las excursiones del sábado, las inumerables comidas y cenas, nocheviejas, etc… Rosa, nunca olvidaré nuestra primera salida "Alcampo", tu cara mirándome fijamente mientras cojeabas y sonaba la música de tu móvil!!! Toda una historia… Y en nochevieja, que bien me lo pasé con mi compañera de cubatas y de "baño que pincha", mis vaqueros aún se acuerdan de aquella noche!! Pablo, mi traductor oficial de los Mojinos!! A ver si podemos ir a otro concierto pronto! Y volver a comer crêpes en Paris! Cara-crêpes! Vic, nada más volver de Japón me iniciaste a tu pasión… los pajaritos!! Las orejas de cerdo vinieron mucho más tarde ;-) Me descubriste este mundo y ahora veo los pajaritos con otro ojo (y otra oreja). Tú crees que otro petirrojo nos dará un concierto privado como el de Cazorla??Mamen, no sé ni por dónde empezar… Nunca olvidaré todo lo que has hecho por mí. Recuerdo al principio cuando casi sin conocernos me invitaste a tu cumpleaños junto a toda tu familia!. He disfrutado mucho todos los momentos que hemos pasado juntas estos años (entre las anguilas, el laboratorio, las vacaciones, las fallas, las nocheviejas, los fin des, paseos con bolt, cenas improvisadas...) Creo que he pasado más tiempo contigo que con Richard. Gracias por todo!!! También quiero dar las gracias a una gran cocinera y amiga, llamada Aquagamete, o Pepa, para los íntimos! Me han encantado nuestras comiditas y me ha ayudado mucho tenerte aquí. Además, gracias a ti he podido probar y disfrutar el mejor jamón del mundo! No olvido a los 2 últimos fichados: los vecinos alborayenses Chris y German. Continuaremos disfrutando de nuestras cervecitas y horchatitas después de una partida de volley-playa.Gracias al grupo de nutrición, Miguel, Ana, Silvia, Raquel, Sergio… con mención especial a Guillem por tu ayuda y tu valenciano perfecto (yo tenía complicado el resum en valenciá); y a Nacho, por tu ayuda con la portada y tu paciencia durante el final de mi tesis, ...

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Testis Development, Sperm Quality Evaluation and Cryopreservation in the European Eel

Cited by 11 publications

References 97 publications

Effect of parental origin on early life history traits of European eel

Effect of parental origin on early life history traits of European eel

Evaluation of Methods to Determine Sperm Density for the European eel, Anguilla anguilla

Molecular approaches related to the European eel (Anguilla anguilla) reproductive process

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