Testis Fixation in Prepubertal Rats: Fibrin Glue Versus Transparenchymal Sutures Reduces Testicular Damage

Şencan, Aydın; Genç, Abdülkadir; Günşar, Cüneyt; Dağlar, Zafer; Yılmaz, Özlem; Ulukuş, Çağnur; Özer, Esra Arun; Taneli, Can; E, Mir

doi:10.1055/s-2004-820971

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…Another study mentioned high levels of neutrophil, macrophage infiltration and foreign body reaction after 4 days in the site of retention suture (Ascherman et al, 2005). Sencan et al also reported inflammatory reaction to suture materials with their tissue, where tissue fixed with silk and polypropylene caused more inflammation and destruction of somniferous tubular compared with fibrin glue (Şencan et al, 2004). The Petrut findings showed suture material in the bladder supported intense and moderate tissue reaction with monofilament and Vicryl after 3 weeks respectively (Şencan et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Sencan et al also reported inflammatory reaction to suture materials with their tissue, where tissue fixed with silk and polypropylene caused more inflammation and destruction of somniferous tubular compared with fibrin glue (Şencan et al, 2004). The Petrut findings showed suture material in the bladder supported intense and moderate tissue reaction with monofilament and Vicryl after 3 weeks respectively (Şencan et al, 2004). A notable finding was the increased inflammatory reaction in the control method, specifically in the folded area.…”

Section: Discussionmentioning

confidence: 99%

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A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold

Khorramirouz

Noble

et al. 2018

Acta Histochemica

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Objectives Subcutaneous implantations in small animal models are currently required for preclinical studies of acellular tissue to evaluate biocompatibility, including host recellularization and immunogenic reactivity. Methods Three rat subcutaneous implantation methods were evaluated in six Sprague Dawley rats. An acellular xenograft made from porcine pericardium was used as the tissue-scaffold. Three implantation methods were performed; 1) Suture method is where a tissue-scaffold was implanted by suturing its border to the external oblique muscle, 2) Control method is where a tissue-scaffold was implanted without any suturing or support, 3) Frame method is where a tissue-scaffold was attached to a circular frame composed of polycaprolactone (PCL) biomaterial and placed subcutaneously. After 1 and 4 weeks, tissue-scaffolds were explanted and evaluated by hematoxylin and eosin (H&E), Masson’s trichrome, Picrosirius Red, transmission electron microscopy (TEM), immunohistochemistry, and mechanical testing. Results Macroscopically, tissue-scaffold degradation with the suture method and tissue-scaffold folding with the control method were observed after 4 weeks. In comparison, the frame method demonstrated intact tissue scaffolds after 4 weeks. H&E staining showed progressive cell repopulation over the course of the experiment in all groups with acute and chronic inflammation observed in suture and control methods throughout the duration of the study. Immunohistochemistry quantification of CD3, CD 31, CD 34, CD 163, and αSMA showed a statistically significant differences between the suture, control and frame methods (P < 0.05) at both time points. The average tensile strength was 4.03 ± 0.49, 7.45 ± 0.49 and 5.72 ± 1.34 (MPa) after 1 week and 0.55 ± 0.26, 0.12 ± 0.03 and 0.41 ± 0.32 (MPa) after 4 weeks in the suture, control, and frame methods; respectively. TEM analysis showed an increase in inflammatory cells in both suture and control methods following implantation. Conclusion Rat subcutaneous implantation with the frame method was performed with success and ease. The surgical approach used for the frame technique was found to be the best methodology for in vivo evaluation of tissue engineered acellular scaffolds, where the frame method did not compromise mechanical strength, but it reduced inflammation significantly.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold

Khorramirouz

Noble

et al. 2018

Acta Histochemica

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“…Villet R et al, in a study on 40 female rats undergoing partial ovariotomy, assessed the effects of fibrin glue and suture material and observed that atrophic and fibrotic changes were minimal in fibrin glue group as compared to suture group[17]. Sencan A et al studied the effect of fibrin glue as compared to transparenchymal suture fixation in 28 Westar rats[18]. In their study, only the testicular inflammation and seminiferous tubular diameter were assessed.…”

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confidence: 99%

Testicular fixation and its effect on ipsilateral and contralateral testis in prepubertal rat model

2021

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Background While performing orchidopexy, various suture materials or fibrin glues are used to achieve testicular fixation. This study was designed to assess the histological changes in testis after orchidopexy using fibrin glue and suture material. Methods Male Wistar rats (n = 80) were divided randomly into four groups. Group I, (n = 20): sham operation, Group II (n = 20): Dartos Pouch (DP), Group III (n = 20): Transtunical fixation (TF), Group IV (n = 20): Tissue Adhesive (TA). Ipsilateral and contralateral testicular histology was assessed at 70 and 120 days of life after sacrificing animals by using thiopental sodium intraperitoneally at a dose of 100 mg/kg. Results Morphologically, at day 70, contralateral testis in Group III had a significant (p value 0.046) decrease testicular width (0.92 ± 0.01 vs 1.24 ± 0.39 cm). At 120 of life, Group I, II, III, and IV had a significant (p value < 0.001 each) decrease testicular width and weight in ipsilateral and decrease testicular length (p value 0.002) in contralateral testis. Histologically, mean seminiferous tubular diameter and DNA flow cytometry had a significant (p value < 0.001) decrease in size in Group I, II, III, and IV both ipsilateral as well as contralateral testis. Intergroup comparison at 70 and 120 days of life showed a significant decrease in seminiferous tubular diameter in Group II, III and IV and in Johnsen maturation score, seminiferous tubular diameter, DNA flow cytometry in Group I, II, III, and IV. Conclusions Dartos Pouch is most suitable procedure for treatment of orchidopexy. Suture fixation must be avoided and if the need arises then instead of suture materials, fibrin glue should be used for testicular fixation.

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Orchidopexy and orchidectomy

Hutton¹,

Darwish²

2015

Pediatric Urology

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Testis Fixation in Prepubertal Rats: Fibrin Glue Versus Transparenchymal Sutures Reduces Testicular Damage

Cited by 6 publications

References 5 publications

A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold

A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold

Testicular fixation and its effect on ipsilateral and contralateral testis in prepubertal rat model

Orchidopexy and orchidectomy

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