Testis Glutathione Peroxidase and Phospholipid Hydroperoxide Glutathione Peroxidase Activities in Aminoguanidine-Treated Diabetic Rats

Ünlüçerçi, Yeşim; Bekpinar, Selda; Koçak, Hikmet

doi:10.1006/abbi.2000.1876

Cited by 26 publications

(10 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the group treated with AMG, the blood glucose values significantly decreased compared to those of the STZ group. These results are in line with other works (20,21) like that of Liptakov et al (19) which report meaningful decline in plasma glucose levels following the 8-week AMG treatment applied to diabetic rats.…”

Section: Resultssupporting

confidence: 93%

Anti-Apoptotic Effects of Aminoguanidine Against Liver Damage on Experimental Diabetes in Rats

2014

J Turgut Ozal Med Cent

View full text Add to dashboard Cite

Objective: This study was designed to investigate the antiapoptotic and improving effects of aminoguanidine on the histological alterations in liver in streptozotocin (STZ)-induced diabetic rat model. Material and Methods: 32 male Sprague Dawley rats were divided into the following 4 groups: Control, Aminoguanidine (AMG), Streptozotocin (STZ), Streptozotocin + Aminoguanidine (STZ+AMG). The animals in group STZ and STZ+AMG were made diabetic by intraperitoneal injection of streptozotocin 45 mg/kg. Histochemical and immunohistochemical staining methods were applied to sections obtained from paraffin blocks and preparations were examined by using Leica DFC-280 light microscope. Results: The sections taken from the control and AMG groups were normal in histological appearance. However, multifocal nodules containing inflammatory cells were observed in the diabetic group. Moreover necrotic hepatocytes and hemorrhagic areas were observed among intact hepatocyte of lobul in the STZ group. Glycogen loss was observed in the hepatocytes localized at the periphery of lobule. In addition, the numbers of cells with positive staining by caspase-3 were significantly increased. On the other hand, it was seen that, in the groups administered with STZ, histological injury in the liver was attenuated by the administration of AMG. Moreover, it was found that the number of caspase-3 positive cells was significantly decreased in the STZ+AMG group. Conclusion: We concluded that chronic aminoguanidine administration reduced liver injury in STZ-induced diabetic rats. Thus, we suggest that aminoguanidine may be used to prevent the development of diabetic liver damage. Key Words: Aminoguanidine; Diabetes mellitus; Streptozotocin; Rat.Deneysel Diyabet Oluşturulan Sıçanlarda Meydana Gelen Karaciğer Hasarına Karşı Aminoguanidinin Antiapoptotik Etkileri Özet Amaç: Bu çalışma, streptozotosin (STZ) ile oluşturulan diyabetik sıçan modelinde, karaciğerde ortaya çıkan histolojik değişiklikler üzerine aminoguanidinin antiapoptotik ve iyileştirici etkilerinin araştırılması amacıyla planlandı. Gereç ve Yöntemler: Çalışmada 32 adet Sprague Dawley erkek sıçan kullanıldı. Sıçanlar her biri 8 adetten oluşan 4 gruba ayrıldı. Kontrol, aminoguanidin (AMG), Streptozosin (STZ), Streptozosin+AMG (STZ+AMG). Deneysel diyabet STZ ve STZ+AMG gruplarında tek doz STZ'nin (45 mg/kg) intraperitoneal (i.p.) uygulanması ile oluşturuldu. Alınan dokular, rutin doku takip işlemlerinden geçirilerek parafine gömüldü. Parafin bloklardan alınan kesitlere histokimyasal (Mayer's Hemotoksilen-Eozin, PAS) ve immunohistokimyasal (kaspaz-3) boyama yöntemleri uygulandı. Boyanan kesitler ve Leica DFC-280 ışık mikroskobunda incelendi. Bulgular: Kontrol ve AMG grubu normal histolojik görünümdeydi. Bununla birlikte STZ grubunda parankima içerisinde inflamatuvar hücre içeren multifokal nodüller izlendi. Ayrıca sağlam hepatositler arasında yerleşmiş asidofil sitoplazmalı ve heterokromatik nükleuslu nekrotik hücrelere ve hemorajik alanlara rastlandı. Lobülün periferinde yer alan hepa...

show abstract

Section: Resultssupporting

confidence: 93%

Anti-Apoptotic Effects of Aminoguanidine Against Liver Damage on Experimental Diabetes in Rats

2014

J Turgut Ozal Med Cent

View full text Add to dashboard Cite

show abstract

“…There are conflicting results regarding with the effect of DM on ROS production in the testis. According to Ü nlüçerçi et al (25), oxidative stress is not the main determinant of testicular dysfunction and complications in DM. Nazirog˘lu (26) demonstrated elevated ROS levels and the protective effect of vitamin E and C and selenium in diabetic rat testis.…”

Section: Discussionmentioning

confidence: 98%

The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress

Aybek

Rota

et al. 2008

Fertility and Sterility

View full text Add to dashboard Cite

“…Diabetes results in certain changes in seminiferous tubules, including increased tubular wall thickness, severe germ-cell depletion and Sertoli cell vacuolization, as well as reduced testicular volumes, semen volume and numbers of Leydig cells and their spontaneous secretion of testosterone (16). Oxidative stress plays a direct role in the pathogenesis of various diabetic complications, which is one of the major causes of testicular dysfunction (1,17).…”

Section: Discussionmentioning

confidence: 99%

Effects of apocynin on oxidative stress and expression of apoptosis-related genes in testes of diabetic rats

Liu

et al. 2012

Molecular Medicine Reports

View full text Add to dashboard Cite

Reactive oxygen species (ROS) are important in the development of diabetic testicular dysfunction. Overproduction of ROS promotes the process of apoptosis, which shows that there is a crosstalk between oxidative stress and apoptosis. Recent research has suggested that NADPH oxidase is the main source of ROS. In this study, we investigated whether the NADPH oxidase inhibitor, apocynin, can improve diabetes‑induced testicular dysfunction by suppressing oxidative stress. The streptozocin (STZ)-induced diabetic rats were administered apocynin, and the mRNA and protein expression of Bax, Bcl-2, p47phox and p67phox was examined by real-time PCR (RT-PCR) and western blot analysis. Production of ROS was measured by thiobarbituric acid reactive substances (TBARS) assay. Terminal-deoxynucleoitidyl transferase mediated nick end-labeling (TUNEL) assay was used to detect apoptosis and ELISA was used to detect total testosterone levels. The mRNA and protein expression of Bcl-2 was signiﬁcantly reduced, and that of Bax, p47phox and p67phox was signiﬁcantly increased in the diabetic rats compared to the control group. Apocynin signiﬁcantly increased the expression of Bcl-2 and decreased the expression of Bax, p47phox and p67phox at both the mRNA and protein levels. The production of ROS and apoptotic cells signiﬁcantly increased in the diabetic group compared to the control group. Apocynin signiﬁcantly reduced the production of ROS and apoptotic cells and increased the total testosterone level. In conclusion, apocynin is capable of ameliorating testicular dysfunction.

show abstract

Testis Glutathione Peroxidase and Phospholipid Hydroperoxide Glutathione Peroxidase Activities in Aminoguanidine-Treated Diabetic Rats

Cited by 26 publications

References 24 publications

Anti-Apoptotic Effects of Aminoguanidine Against Liver Damage on Experimental Diabetes in Rats

Anti-Apoptotic Effects of Aminoguanidine Against Liver Damage on Experimental Diabetes in Rats

The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress

Effects of apocynin on oxidative stress and expression of apoptosis-related genes in testes of diabetic rats

Contact Info

Product

Resources

About