Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets

Ikhwanuddin, Mhd; Azmie, Ghazali; Nahar, S; Wee, Wendy; Azra, Mohamad Nor; Abol‐Munafi, Ambok Bolong

doi:10.17576/jsm-2018-4703-01

Cited by 22 publications

(8 citation statements)

References 13 publications

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“…DEGs between adults and juveniles of the same sex were less than those between males and females, while the number of DEGs (Ov0 vs. Ov4) functioning in the maturation of the ovary was more than that of the testis. This phenomenon can be explained by the reality that ovary development goes through more developmental stages, which is more complicated than the testis (Mhd et al, 2018;Wu et al, 2019a). The functional classifications in GO and KEGG of DEGs between testis (Te0 vs. Te2) and ovary (Ov0 vs. Ov4) maturation demonstrated the differences in the gonadal developmental regulatory pathways.…”

Section: Discussionmentioning

confidence: 99%

“…The mature testis at stage 2 (Te 2) and ovary at stage 4 (Ov 4) were obtained from 12 individuals (six individuals of each sex), and immature testis (Te 0) and ovary (Ov 0) from 12 juvenile crabs (six individuals of each sex) were excised for SMRT sequencing and RNA-Seq. At stage 2, testes are swollen, opaque, and white; vas deferens are swollen and pink, contains spermatophore, which is ready for mating (Mhd et al, 2018). As for the stage 4 ovary, which is in the late-maturing or secondary vitellogenic (stage 4) stage, the oocyte grows rapidly, and the nucleus reached the maximum at this stage or the end of primary vitellogenesis (Islam et al, 2010).…”

Section: Animal Collection and Rna Extractionmentioning

confidence: 99%

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Integrative Transcriptome Sequencing Reveals the Molecular Difference of Maturation Process of Ovary and Testis in Mud Crab Scylla paramamosain

et al. 2021

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The mud crab Scylla paramamosain is a species with significant sexual dimorphism in growth rate and body size, of which the females are of higher economic and nutritional values than the males. Accordingly, there is an urgent need to explore the molecular mechanism underlying sex determination and gonadal differentiation. The single-molecule long-read technology combining with RNA sequencing was employed to construct a full-length transcriptome for gonads of S. paramamosain. In total, 1,562,819 FLNC reads were obtained from 1,813,758 reads of inserts (ROIs). Among them, the 10,739 fusion isoforms corresponded to 23,634 reads and were involved in 5,369 genes in the reference annotation. According to the criteria for new transcripts, a total of 213,809 isoforms were recognized as novel transcripts and then matched against Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), NR, Swissprot, and KOG databases. We also identified 22,313 SSRs, 169,559 lncRNAs, and 25,451 SNPs. Additionally, 349,854 alternative splicing (AS) events from 8,430 gene models were detected, and 5,129 polyadenylation sites were profiled from 3,090 genes. GO and KEGG annotation indicated that AS and APA probably play important roles in the gonadal development and maturation. Besides, the DEGs associated with gonadal development and maturation were identified and analyzed based on the RNA-Seq data.

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Section: Discussionmentioning

confidence: 99%

Section: Animal Collection and Rna Extractionmentioning

confidence: 99%

Integrative Transcriptome Sequencing Reveals the Molecular Difference of Maturation Process of Ovary and Testis in Mud Crab Scylla paramamosain

et al. 2021

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“…In addition, several species are restricted to certain environments that affect the feeding selection between species and between life stages [19][20][21][22]. For example, mangrove crabs or mud crabs (Scylla spp.…”

Section: Decapod Feeding Biologymentioning

confidence: 99%

Use of Pelleted Diets in Commercially Farmed Decapods during Juvenile Stages: A Review

Aaqillah‐Amr

Hidir

Azra

et al. 2021

Animals

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The increasing market demand for decapods has led to a considerable interest in cultivating decapod species at a larger scale. Following the development of hatchery technologies, most research has focused on the development of formulated feeds for commercially farmed decapods once they enter the juvenile stages. The use of formulated feed for decapods at a commercial scale is still in the early stages. This is probably because of the unique feeding behavior that decapods possess: being robust, slow feeders and bottom dwellers, their feeding preferences change during the transition from pelagic larvae to benthic juveniles as their digestive systems develop and become more complex. The current practice of decapod aquaculture involves the provision of juveniles with food such as natural diet, live feed, and formulated feed. Knowledge of nutrient requirements enables diets to be better formulated. By manipulating the levels of proteins and lipids, a formulated feed can be expected to lead to optimal growth in decapods. At the same time, the pellet’s physical characteristics are important factors to be considered upon formulating commercially farmed decapod feeds, considering the unique feeding behavior of the decapod. However, most published studies on decapod nutrition lack data on the physical characteristics of the feed types. Thus, it is difficult to establish a standard feed formulation that focuses on the physical pellet properties. Moreover, careful consideration must be given to the feeding behavior of species, as decapods are known as bottom feeders and are robust in terms of handling feed. Information on the pellet forms, diet composition, and unique feeding behaviors in commercially farmed decapods is gathered to suggest potential better formulated diets that can optimize growth and reproduction. Thus, the purpose of this review is to summarize the information that has been published to date and to come up with suggestions on ways to improve the feed formulation in decapods that comply with their feeding behavior and nutrient requirements. Further research is needed to explore the potential of the pelleted feed at the adult stage so the decapod can take full advantage of the nutrients present in the pellets.

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“…The demand for this species increases annually [3], which has led to overfishing, as most mud crab landings still rely heavily on captured fisheries [4,5]. The impact on wild populations could be lessened, if sufficient support were received from the aquaculture sector in the form of seed supply from hatcheries [2,6,7]. As well, mud crab grow-out and fattening activities incorporated within the mangrove area, known as silvo-fishery techniques [8,9,10], culture in earthen ponds [11,12] or in concrete ponds [13], and grow-out activity in indoor conditions [14,15], could all contribute to hatchery replacement of wild catch.…”

Section: Introductionmentioning

confidence: 99%

Nursery Culture of Mud Crab, Genus Scylla, a Review: The Current Progress and Future Directions

Syafaat¹,

Azra²,

Waiho³

et al. 2021

Preprint

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This study attempted to explain the definition, objectives, and key stages in mud crab nursery activities. The nursery stages of Scylla spp was started from megalopa stage to several crablet instar stages or started from earlier crablet stage to several crablet stages. Direct stocking of megalopa into ponds is not recommended due to their sensitivity. Instead, nursery rearing is needed to produce mud crab of larger size before stocking. Individual nursery rearing results in higher survival rate (up to 100%), but with reduced growth rate, and a more complicated maintenance process compared with communal rearing. Nursery of mud crab can be done indoors, or outdoors, with adequate shelter and feed required to obtain good survival and growth performance. Artemia nauplii are the main, established nursery feed, particularly at megalopa stage, while survival rate may be improved if combined with artificial feed, such as micro bound diet formulations. Water quality parameters, identical to those proposed in tiger shrimp culture, can followed. Crablets may be transported to the pond location with, or without water. The provision of monosex seeds from mud crab hatcheries is expected to become more common, increasing seed price, and thus improving income of farmers. Numerous aspects of mud crab nursery, including nutrition, feeding strategies, understanding of their behaviour, i.e., cannibalism, control of environmental factors, and practical rearing techniques, still need further improvement.

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Testis Maturation Stages of Mud Crab (Scylla olivacea) Broodstock on Different Diets

Abstract: The aim of this study was to investigate how various diets influence testis maturation stages in mud crab

Cited by 22 publications

References 13 publications

Integrative Transcriptome Sequencing Reveals the Molecular Difference of Maturation Process of Ovary and Testis in Mud Crab Scylla paramamosain

Integrative Transcriptome Sequencing Reveals the Molecular Difference of Maturation Process of Ovary and Testis in Mud Crab Scylla paramamosain

Use of Pelleted Diets in Commercially Farmed Decapods during Juvenile Stages: A Review

Nursery Culture of Mud Crab, Genus Scylla, a Review: The Current Progress and Future Directions

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