Testosterone in human urine

Futterweit, Walter; McNiven, Neal L.; Guerra-García, Roger; Gibree, Norman B.; M, Drosdowsky; Siegel, George L.; Soffer, Louis J.; Rosenthal, Ira M.; Dorfman, Ralph I.

doi:10.1016/0039-128x(64)90031-5

Cited by 30 publications

(6 citation statements)

References 15 publications

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“…The urinary excretion noted for total and free testosterone, progesterone, and estradiol was of the same order as reported by other authors [5][6][7][8][9][10][11][12][13][14]. The variation within an individual for the urinary excretion of testosterone, progesterone and estradiol was appreciably greater than for the variation in serum.…”

Section: Reply To Comment On "Isolation and Detection Of Steroids Frosupporting

confidence: 84%

“…Hence, an analysis of these hormones in urine requires complete hydrolysis of the known conjugates and sulphates. The results in this table refer to spiked urine samples.The urinary excretion noted for total and free testosterone, progesterone, and estradiol was of the same order as reported by other authors [5][6][7][8][9][10][11][12][13][14]. The problem with this treatment is in its effectiveness, because many steroid forms are resistant to treatment or prefer only one type of hydrolysis (e.g., sulphates prefer only solvolysis).…”

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confidence: 80%

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Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

Gadzała‐Kopciuch

Ričanyová

Buszewski

2010

Journal of Chromatography B

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Section: Reply To Comment On "Isolation and Detection Of Steroids Frosupporting

confidence: 84%

supporting

confidence: 80%

Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

Gadzała‐Kopciuch

Ričanyová

Buszewski

2010

Journal of Chromatography B

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“…Futterweit et al were one of the first to set a reference range for testosterone in urine, using thin layer chromatography (TLC) and gas chromatography (GC) [26]. Doberne and New and Tresguerres et al on the other hand used a, at that time, very new and high-tech isotopically labelled ligand binding assay (Radioimmunoassay, RIA) [27,28].…”

Section: Reference Ranges For Aas In Humans and Horsesmentioning

confidence: 99%

Mass Spectrometry for the Detection of Endogenous Steroids and Steroid Abuse in (Race) Horses and Human Athletes

Anneleen¹,

Anita²,

Vanhaecke³

2017

Mass Spectrometry

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The higher the pressure to win, the more athletes are inclined to take steps to improve one's performance through questionable means. To minimize this, strict anti-doping and medication rules are being enforced. All human and equine athletes are regularly subjected to doping analysis to prevent abuse of forbidden substances from affecting their performance. Anabolic-androgenic steroids (AASs) have been part of the forbidden substances list for years, because of their muscle building and performance-enhancing capacities and possible side effects. For most of the AAS, zero-tolerance is held. However, some AASs can be endogenous to the athletes, such as for example testosterone in males. These endogenous steroids can render it very difficult to reveal steroid abuse. Specific mass spectrometric (MS) methods, including ultra-high performance liquid chromatography-MS (UHPLC-MS/MS), high resolution mass spectrometry (HRMS) and gas chromatography-combustion-isotope ratio MS (GC-C-IRMS), have been put forward to overcome these analytical difficulties. Currently, high-tech metabolomic methods are being used to build athlete specific biological passports. In the near future, these passports might allow putting a stop to abuse, by staying ahead of the cheats. These are bright prospects, leading towards clean and fair sports competitions worldwide.

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“…In this subject, estrogen production rates, the urinary excretion of estrogen and of testosterone, and the conversion of intravenously administered androstenedione and testosterone into estrogen are detailed together with more conventional observations. In addition, 8 other instances of the syndrome not included in the previous report are reviewed.…”

Section: Ecentlymentioning

confidence: 99%

Feminizing adrenocortical carcinoma in a man

Gabrilove¹,

Nicolis²,

Hausknecht³

et al. 1970

Cancer

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A second instance of a feminizing adrenocortical tumor studied in this laboratory is reported. A marked increase in the production rates of estrone a n d estradiol was demonstrated as well as in the urinary excretion of estogens.These latter were partitioned into 16-hydroxyestrone, 16-ketoestradiol, a n d 16-epiestriol as well as the classical estrogens. The secretory rate of cortisol was low whereas that of 11-deoxycortisol was markedly increased. The urinary excretion of testosterone was normal in spite of marked feminization and atrophy of the testes. Of interest was the demonstration of the marked conversion into estrogen of androstenedione and testosterone administered intravenously. I n addition, 8 further instances of feminizing adrenocortical carcinoma collected from the literature are reviewed. ECENTLYWE REVIEWED rHE CLINICAL, R pathologic, and biochemical features of 52 men with feminizing adrenocortical tumors.10 In the present study, we report a second such patient investigated in this laboratory. In this subject, estrogen production rates, the urinary excretion of estrogen and of testosterone, and the conversion of intravenously administered androstenedione and testosterone into estrogen are detailed together with more conventional observations. In addition, 8 other instances of the syndrome not included in the previous report are reviewed. MATERIALS AND METHODSThe urinary excretion of the neutral 17ketosteroids was measured by the method of Drekter et a1.0 and of the 17-hydroxycor-From the Endocrine Research ticoids by the Peterson":' modification of the method of Silber and Porter.28 Urinary estrogens were assayed by gas chromatography.33 Estrogen production rates were measured according to the procedure of Siiteri and Mac-Donald." The secretory rates of cortisol and 1 I-deoxycortisol were determined as previously described." Urinary testosterone was measured' by a modification of the method of Futterweit et a1.8.11 Tracer doses of androstenedione-sH and testosterone-3H were injected intravenously on separate occasions. The urine was collected for 3 days and the percent distribution of radioactivity in the estrone and estriol fractions was measured after purification to constant specific activity. Conversions of the radioactive androgens into estrone and estriol were calculated by correcting these results for the recovery of rad'ioactivity obtained after the intravenous injection of estrone-14C and estradiol-3H during the production rate studies. This indirect correction was necessary because carbon-1 4-labelled estrogens were unfortunately not given concomitantly with the tritium-labelled androgens. CASE REPORTA 55-year-old Negro man was first admitted to The Mount Sinai Hospital on Aug. 6, 1967, because of gynecomastia and a left upper quadrant mass. He stated that although he had noted the onset of secondary sexual characteristics with pubic hair at the age of 17 years and axillary hair at 20 years of 153

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Testosterone in human urine

Cited by 30 publications

References 15 publications

Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

Reply to comment on “Isolation and detection of steroids from human urine by molecularly imprinted solid-phase extraction and liquid chromatography” by Tse Sum Bui and Haupt [J. Chromatogr. B 877 (2009) 4180–4181]

Mass Spectrometry for the Detection of Endogenous Steroids and Steroid Abuse in (Race) Horses and Human Athletes

Feminizing adrenocortical carcinoma in a man

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