Testosterone replacement attenuates intimal hyperplasia development in an androgen deficient model of vascular injury

Freeman, Brian M.; Univers, Junior; Fisher, Richard K.; Kirkpatrick, Stacy S.; Klein, Frederick; Freeman, Michael B.; Mountain, Deidra J.H.; Grandas, Oscar H.

doi:10.1016/j.jss.2016.08.016

Cited by 9 publications

(5 citation statements)

References 42 publications

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“…The presence of E 2 (see Figure 3) was not observed on the first nine sampling dates (19 days) of the experiment. On successive sampling dates (15 dates, 53.33%), E 2 values were higher in both E groups than in group C. The concentrations of T (see Figure 4) were lower on 60% sampling dates in both experimental groups than in the control group, which provides indirect evidence for the acceleration of metabolic processes or the conversion of T to E 2 [39] under the suppressive influence of ZEN [34,[40][41][42]. The concentrations of E 2 after 20 days of exposure and serum T levels throughout the experiment were directly proportional to the ZEN dose.…”

Section: Concentrations Of Estradiol and Testosteronementioning

confidence: 83%

“…According to Vandenberg et al [22], ZEN and/or α-ZEL exert a "complementary" effect on the concentrations of endogenous hormones (see Figures 3 and 4). The above is reflected by an increase in E 2 levels from day 20 to day 42 of the experiment and the decreasing trend in the concentrations of T between day 2 and the end of the experiment, which could alleviate disruptions in hormonal homeostasis [40,41]. The observed T values could also be attributed to the fact that T regulates sexual differentiation, muscle and bone mass, and erythropoietic and metabolic processes which play a very important role in the development of pre-pubertal gilts.…”

Section: Concentrations Of Estradiol and Testosteronementioning

confidence: 99%

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The Effect of Different Doses of Zearalenone in Feed on the Bioavailability of Zearalenone and Alpha-Zearalenol, and the Concentrations of Estradiol and Testosterone in the Peripheral Blood of Pre-Pubertal Gilts

Zielonka

Gajęcka

Lisieska-Żołnierczyk³

et al. 2020

Toxins

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The objective of this study was to determine the effect of long-term (48 days), per os administration of specific zearalenone (ZEN) doses (20 and 40 μg ZEN/kg BW in experimental groups EI and EII, which were equivalent to 200% and 400% of the upper range limit of the no-observed-adverse-effect-level (NOAEL), respectively) on the bioavailability of ZEN and the rate of changes in estradiol and testosterone concentrations in the peripheral blood of pre-pubertal gilts. ZEN and α-ZEL levels were similar until day 28. After day 28, α-ZEL concentrations increased significantly in group EI, whereas a significant rise in ZEN levels was noted in group EII. The presence of estradiol in peripheral blood plasma was not observed until day 20 of the experiment. Spontaneous secretion of estradiol was minimal, and it was determined at very low levels of up to 10 pg/mL in EI and EII groups. Testosterone concentrations ranged from 4 to 9 ng/mL in all groups. A decrease in the concentrations of both analyzed hormones was reported in the last stage of the experiment. The results of the experiment indicate that: (i) The bioavailability of ZEN in peripheral blood has low diagnostic value, (ii) exposure to low doses of ZEN induces minor changes in the concentrations of the analyzed hormones, which could lead to situational supraphysiological hormone levels and changes in endogenous hormonal balance.

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Section: Concentrations Of Estradiol and Testosteronementioning

confidence: 83%

Section: Concentrations Of Estradiol and Testosteronementioning

confidence: 99%

The Effect of Different Doses of Zearalenone in Feed on the Bioavailability of Zearalenone and Alpha-Zearalenol, and the Concentrations of Estradiol and Testosterone in the Peripheral Blood of Pre-Pubertal Gilts

Zielonka

Gajęcka

Lisieska-Żołnierczyk³

et al. 2020

Toxins

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“…Steroid hormones, including androgens, are involved in many developmental and physiological processes [ 1 – 4 ]. Classically androgens mediate their effects via binding to the androgen receptor (AR), a ligand-dependent transcription factor and a member of the nuclear receptor gene superfamily [ 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic and non-genomic effects of androgens in the cardiovascular system: clinical implications

et al. 2017

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The principle steroidal androgens are testosterone and its metabolite 5α-dihydrotestosterone (DHT), which is converted from testosterone by the enzyme 5α-reductase. Through the classic pathway with androgens crossing the plasma membrane and binding to the androgen receptor (AR) or via mechanisms independent of the ligand-dependent transactivation function of nuclear receptors, testosterone induces genomic and non-genomic effects respectively. AR is widely distributed in several tissues, including vascular endothelial and smooth muscle cells. Androgens are essential for many developmental and physiological processes, especially in male reproductive tissues. It is now clear that androgens have multiple actions besides sex differentiation and sexual maturation and that many physiological systems are influenced by androgens, including regulation of cardiovascular function [nitric oxide (NO) release, Ca2+ mobilization, vascular apoptosis, hypertrophy, calcification, senescence and reactive oxygen species (ROS) generation]. This review focuses on evidence indicating that interplay between genomic and non-genomic actions of testosterone may influence cardiovascular function.

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“…The vascular reactivity curve was undertaken and the vessel was washed before the next curve and then reincubated with the steroid again for 30 minutes. The doses for the sex steroids were chosen because they had previously been investigated using dose-response curves by members of the same laboratory group ( 23 , 24 ) and were in line with those reported in vascular tissue in the recent scientific literature ( 25 , 26 ). Importantly, a literature review before the experiments beginning suggested that higher doses of estrogen and dihydrotestosterone (DHT) in particular could inhibit VSMC proliferation and action ( 27 ).…”

Section: Methodsmentioning

confidence: 99%

Effects of Sex Hormones on Vascular Reactivity in Boys With Hypospadias

Lucas-Herald,

Montezano,

Alves-Lopes

et al. 2023

The Journal of Clinical Endocrinology &Amp; Metabolism

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Background Arteries from boys with hypospadias demonstrate hypercontractility and impaired vasorelaxation. The role of sex hormones in these responses in unclear. Aims We compared effects of sex steroids on vascular reactivity in healthy boys and boys with hypospadias. Methods Excess foreskin tissue was obtained from 11 boys undergoing hypospadias repair (cases) and 12 undergoing routine circumcision (controls) (median age (range) 1.5 (1.2, 2.7) yrs) and small resistance arteries were isolated. Vessels were mounted on wire myographs and vascular reactivity was assessed in the absence/presence of 17β-estradiol, dihydrotestosterone (DHT) and testosterone. Results In controls, testosterone and 17β-estradiol increased contraction (Emax: 83.74 basal vs 125.4 after testosterone, p < 0.0002 and 83.74 vs 110.2 after estradiol, p = 0.02). 17β-estradiol reduced vasorelaxation in arteries from controls (Emax: 10.6 vs 15.6 to acetylcholine (ACh), p < 0.0001 and Emax: 14.6 vs 20.5 to sodium nitroprusside (SNP), p < 0.0001). In hypospadias, testosterone (Emax: 137.9 vs 107.2, p = 0.01) and 17β-estradiol (Emax: 156.9 vs 23.6, p < 0.0001) reduced contraction. Androgens, but not 17β-estradiol, increased endothelium-dependent and endothelium-independent vasorelaxation in cases (Emax: 77.3 vs 51.7 with testosterone, p = 0.02 and vs 48.2 with DHT to ACh, p = 0.0001; Emax: 43.0 vs 39.5 with testosterone, p = 0.02 and vs 39.6 vs 37.5 with DHT to SNP, p = 0.04). Conclusion In healthy boys, testosterone and 17β-estradiol promote a vasoconstrictor phenotype whereas in boys with hypospadias, these sex hormones reduce vasoconstriction, with androgens promoting vasorelaxation. Differences in baseline artery function may therefore be sex hormone independent and the impact of early-life variations in androgen exposure on vascular function needs further study.

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Testosterone replacement attenuates intimal hyperplasia development in an androgen deficient model of vascular injury

Cited by 9 publications

References 42 publications

The Effect of Different Doses of Zearalenone in Feed on the Bioavailability of Zearalenone and Alpha-Zearalenol, and the Concentrations of Estradiol and Testosterone in the Peripheral Blood of Pre-Pubertal Gilts

The Effect of Different Doses of Zearalenone in Feed on the Bioavailability of Zearalenone and Alpha-Zearalenol, and the Concentrations of Estradiol and Testosterone in the Peripheral Blood of Pre-Pubertal Gilts

Genomic and non-genomic effects of androgens in the cardiovascular system: clinical implications

Effects of Sex Hormones on Vascular Reactivity in Boys With Hypospadias

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