Tests of Platelet Function

Platelet Function Tests

“…New approaches to LTA, including automation and transfer to 96-well plate-based assays, as well as novel microfluidic assays appear particularly promising for deep phenotyping of platelet responses. 48,53,91 The future of platelet function testing will need to harvest the wealth of data generated by high throughput deep phenotyping approaches and translate these platelet function findings into actionable determinants of disease, with ideally pharmacological targets to influence progression of disease. Platelet function testing is therefore at a particularly interesting stage, as the development of new technologies and new instruments may have future utility in a variety of different clinical and laboratory settings.…”

“…52 This technique can provide a rapid assessment of most platelet activation pathways within minutes, including detailed dose-response curves which are rarely obtainable from LTA due to blood volume restrictions, the number of channels available, and time. 53 The assay is a promising tool for screening of bleeding disorders and monitoring of antiplatelet therapy, as well as understanding basic pathophysiology of platelets. [53][54][55] Whole Blood Aggregometry Whole blood aggregometry (WBA) is based on a change in electrical impedance resulting from platelet aggregation, in response to classical agonists, onto two electrodes immersed directly in saline-diluted whole blood.…”

“…53 The assay is a promising tool for screening of bleeding disorders and monitoring of antiplatelet therapy, as well as understanding basic pathophysiology of platelets. [53][54][55] Whole Blood Aggregometry Whole blood aggregometry (WBA) is based on a change in electrical impedance resulting from platelet aggregation, in response to classical agonists, onto two electrodes immersed directly in saline-diluted whole blood. 56 The Multiplate (F. Hoffmann-La Roche Ltd., Basel, Switzerland) instrument is the most widely used apparatus in clinical laboratories, as it is semiautomated and uses disposable cuvettes/electrodes with preselected agonists for different applications, including diagnosis of bleeding and monitoring of antiplatelet therapy.…”

The Current Role of Platelet Function Testing in Clinical Practice

“…Importantly, the OPTIMUL assay was more sensitive to defects of thromboxane pathway. 49 Overall, results suggested promise as a screening/diagnostic test, especially considering multiple advantages over traditional LTA platforms that include Finally, the development of revolutionary microfluidic flow chambers provides an opportunity to examine coagulation and a complete spectrum of platelet function (adhesion, activation, secretion, aggregation, contraction, and procoagulant activity). The ability to manipulate the system permits the user ability to tailor conditions (type, size, strength of triggering surfaces, flow chamber dimensions, flow rates, flow patterns, wall shear rates, etc.)…”