Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Hansen, Mie Johanne; Pennanen, Elin Anna Erica; Bojesen, Anders Miki; Bertelsen, Mads F.

doi:10.1099/ijsem.0.000759

Cited by 14 publications

(3 citation statements)

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“…Pasteurellaceae species are steadily found to exhibit a commensal lifestyle in the oral cavity of wildlife, including captive marsupials and wild and captive pinnipeds (Hansen et al, 2012(Hansen et al, , 2017, reptiles such as captive turtles (Hansen et al, 2016) and wild Komodo dragons (Montgomery et al, 2002). The study in Komodo dragons is to our knowledge…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, our study generated data on the evolutionary path that microorganisms may endure during the process from wild to captive of their host. Pasteurellaceae species are steadily found to exhibit a commensal lifestyle in the oral cavity of wildlife, including captive marsupials and wild and captive pinnipeds (Hansen et al, 2012, 2017; Hansen, Bertelsen, et al, 2015), reptiles such as captive turtles (Hansen et al, 2016) and wild Komodo dragons (Montgomery et al, 2002). The study in Komodo dragons is to our knowledge the only previous work comparing the oral microbiota of wild and captive animals, where Pasteurellaceae were found sporadically in the wild group only, using a culture‐based approach.…”

Section: Discussionmentioning

confidence: 99%

“…While most members of this family generally have a commensal lifestyle, some species can be highly pathogenic (Bonaventura et al, 2010). Importantly, with the exception of Pasteurella multocida (potential pathogen of multiple phylogenetically unrelated hosts), Pasteurellaceae taxa are associated to a single group of closely related host species (Hansen et al, 2012, 2015, 2016, 2017; Sinclair et al, 2019). Such associations include the closely‐related Ursidibacter maritimus and Ursidibacter arcticus colonising the oral cavity of polar and brown bears, respectively (Hansen, Strom Braaten, et al, 2015), Gallibacterium anatis in the respiratory and genital tract of galliform birds (Johnson et al, 2013), Mannheimia haemolytica in ruminants (Larsen et al, 2007), and Lonepinella sp.…”

Section: Introductionmentioning

confidence: 99%

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Polar bear‐adapted Ursidibacter maritimus are remarkably conserved after generations in captivity

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Most species in the bacterial family of Pasteurellaceae colonize one specific host species. Vertebrates of very different evolutionary descent including fish, turtles, marsupials, eutherians and birds are colonized by different members of Pasteurellaceae. This one‐to‐one microbial‐host species partnership makes Pasteurellaceae species valuable candidates to study biodiversity, bacterial‐host co‐evolution and host adaptation, and their widespread distribution across vertebrates provide the possibility to collect a wide array of data, where wildlife species are essential. However, obtaining samples from wild animals comes with logistic, technical and ethical challenges, and previous microbiota studies have led to the presumption that captive animals are poor models for microbial studies in wildlife. Here, we show that colonization of polar bears by Ursidibacter maritimus is unaffected by factors related to captivity, reflecting a deep symbiotic bond to the host. We argue that the study of ecological and evolutionary principles in captive wildlife is possible for host‐adapted taxa such as those in the Pasteurellaceae family. Moreover, studying captive, often trained animals protects wild populations from the stress associated with obtaining samples.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Polar bear‐adapted Ursidibacter maritimus are remarkably conserved after generations in captivity

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Pasteurellaceae

Kuhnert¹,

Busse²,

Blackall³

et al. 2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Pas.teu.rel.la.ce'ae. N.L. fem. n. Pasteurella type genus of the family; ‐aceae suffix to denote a family; N.L. fem. pl. n. Pasteurellaceae the Pasteurella family. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae Members of the family Pasteurellaceae are Gram‐negative and nonmotile. They are negative for growth on Simmon's citrate agar, and negative tests are observed with mucate acid, malonate base, and growth in KCN. Nitrate is reduced without gas formation, and they are positive in alanine aminopeptidase. (+)‐ D ‐Glucose is metabolized or fermented in the Hugh and Leifson test with the exception of Nicoletella . Members of Pasteurellaceae are negative in arginine dihydrolase and phenylalanine deaminase tests, and tests for hydrolysis of gelatin, Tween, and α‐mannosidase are also negative, and they do not produce acid from meso ‐erythritol, adonitol, (−)‐ L ‐xylose, (+)‐ D ‐fucose, (−)‐ L ‐sorbose, (+)‐ D ‐melezitose, (+)‐ D ‐turanose, and β‐ N ‐CH 3 ‐glucosamine. The family forms a monophyletic group based on 16S rRNA gene and conserved protein sequence comparisons. The genera Aggregatibacter, Avibacterium, Basfia, Bibersteinia, Bisgaardia, Caviibacterium, Chelonobacter, Conservatibacter, Cricetibacter, Frederiksenia, Gallibacterium, Glaesserella, Histophilus, Lonepinella, Mannheimia, Mesocricetibacter, Muribacter, Necropsobacter, Nicoletella, Otariodibacter, Phocoenobacter, Rodentibacter, Seminibacterium, Testudinibacter, Ursidibacter, Vespertiliibacter , and Volucribacter can be regarded as monophyletic or monotypic, whereas some species of the genera Actinobacillus , Haemophilus , and Pasteurella are still polyphyletic with their type species. The major polar lipid profile consists of phosphatidylethanolamine and phosphatidylglycerol. The dominant fatty acids are C 14:0 , C 16:0 , C 16:1 ω7 c , and C 14:0 3‐OH/C 16:1 ISO‐I. Demethylmenaquinones, menaquinones, and ubiquinones with chain lengths of seven or/and eight may be produced. Members of the family have been found with the polyamines putrescine, diaminopropane, cadaverine, spermidine, tyramine, spermine, sym‐norspermidine, and sym‐ homospermidine. Members of Pasteurellaceae are associated with animals and human beings as commensals, opportunistic pathogens, or as primary pathogens. Some have clinical importance, and the distribution seems global. The energy metabolism is chemoheterotrophic, and they are facultative anaerobic, microaerophilic, or aerobic. Type genus : Pasteurella Trevisan 1887 AL (nom. cons. Opinion 13 1954) emend. Mutters et al. 1985b VP . DNA G + C content (mol%) : 36–49.

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Testudinibacter

Hansen¹

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Tes.tu.di.ni.bac'ter. N.L. pl. n. Testudines , taxonomic name for the order of turtles and tortoises; N.L. masc. n. bacter a rod; N.L. masc.n. Testudinibacter , rod isolated from Testudines . Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Testudinibacter Nonmotile, fermentative, Gram‐negative rods that are catalase and oxidase positive. Positive reactions are obtained for indole, ornithine decarboxylase, and Voges–Proskauer, and negative reactions are obtained for methyl red, alkaline phosphatase, urease, and PNPG (α‐glucosidase). Do not require X‐ or V‐factors. Nitrates are reduced to nitrites, but nitrites are not reduced. Acid is produced from l ‐arabinose, dulcitol, d ‐mannitol, l ‐rhamnose, d ‐sucrose and trehalose. The main fatty acids for the strain ELNT2x T are C 14:0 , C 16:1 ω7 c , C 16:0 , C 18:1 ω9 c and summed feature C 18:2 ω6,9 c /ante‐iso‐C 18:0 . ELNT2x T only expressed one respiratory quinone, ubiquinone‐8 (100%). Testudinibacter has been isolated from the oral cavity of several species of freshwater turtles. The pathogenic potential is unknown. DNA G + C content (mol%) : 42.8 (ELNT2x T ). Type species : Testudinibacter aquarius Hansen et al. 2016 VP .

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Testudinibacter aquarius gen. nov., sp. nov., a member of the family Pasteurellaceae isolated from the oral cavity of freshwater turtles

Cited by 14 publications

References 44 publications

Polar bear‐adapted Ursidibacter maritimus are remarkably conserved after generations in captivity

Polar bear‐adapted Ursidibacter maritimus are remarkably conserved after generations in captivity

Pasteurellaceae

Testudinibacter

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