Tethered growth factors on biocompatible scaffolds improve stemness of cultured rat and human neural stem cells and growth of oligodendrocyte progenitors

Moore, Lisamarie; Skop, Nolan B.; Rothbard, Deborah E.; Corrubia, Lucas; Levison, Steven W.

doi:10.1016/j.ymeth.2017.08.015

Cited by 13 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Collagen was used as a control sample due to its well-established role as a scaffold material in tissue engineering [ 49 , 50 , 51 ]. Although both chitosan and collagen have been applied in various tissue culture substrates, including neural tissues, they possess distinct physical properties and bioactivities on hNSCs [ 51 , 52 , 53 , 54 , 55 , 56 ].…”

Section: Resultsmentioning

confidence: 99%

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

et al. 2023

View full text Add to dashboard Cite

Human neural stem cells (hNSCs) possess remarkable potential for regenerative medicine in the treatment of presently incurable diseases. However, a key challenge lies in producing sufficient quantities of hNSCs, which is necessary for effective treatment. Dynamic culture systems are recognized as a powerful approach to producing large quantities of hNSCs required, where microcarriers play a critical role in supporting cell expansion. Nevertheless, the currently available microcarriers have limitations, including a lack of appropriate surface chemistry to promote cell adhesion, inadequate mechanical properties to protect cells from dynamic forces, and poor suitability for mass production. Here, we present the development of three-dimensional (3D) chitosan scaffolds as microcarriers for hNSC expansion under defined conditions in bioreactors. We demonstrate that chitosan scaffolds with a concentration of 4 wt% (4CS scaffolds) exhibit desirable microstructural characteristics and mechanical properties suited for hNSC expansion. Furthermore, they could also withstand degradation in dynamic conditions. The 4CS scaffold condition yields optimal metabolic activity, cell adhesion, and protein expression, enabling sustained hNSC expansion for up to three weeks in a dynamic culture. Our study introduces an effective microcarrier approach for prolonged expansion of hNSCs, which has the potential for mass production in a three-dimensional setting.

show abstract

Section: Resultsmentioning

confidence: 99%

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Under normal conditions, PDGF‐A induces oligodendroglial progenitor cell (OPC) migration in an ERK‐dependent mechanism via regulation of actin reorganization and FAK (Singh et al, 2019). Oligodendrocyte progenitors plated onto a multifunctional film containing PDGF proliferated for at least three days without providing soluble growth factors, while inhibiting the expression of the differentiation marker myelin‐basic protein (Moore et al, 2018). In space flight, the PDGF‐β receptor was downregulated in osteoblasts (Akiyama et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Molecular genetic analysis of neural stem cells after space flight and simulated microgravity on earth

Han

Zeger

Tripathi

et al. 2021

Biotech & Bioengineering

View full text Add to dashboard Cite

Understanding how stem cells adapt to space flight conditions is fundamental for human space missions and extraterrestrial settlement. We analyzed gene expression in boundary cap neural crest stem cells (BCs), which are attractive for regenerative medicine by their ability to promote proliferation and survival of cocultured and co‐implanted cells. BCs were launched to space (space exposed cells) (SEC), onboard sounding rocket MASER 14 as free‐floating neurospheres or in a bioprinted scaffold. For comparison, BCs were placed in a random positioning machine (RPM) to simulate microgravity on earth (RPM cells) or were cultured under control conditions in the laboratory. Using next‐generation RNA sequencing and data post‐processing, we discovered that SEC upregulated genes related to proliferation and survival, whereas RPM cells upregulated genes associated with differentiation and inflammation. Thus, (i) space flight provides unique conditions with distinctly different effects on the properties of BC compared to earth controls, and (ii) the space flight exposure induces postflight properties that reinforce the utility of BC for regenerative medicine and tissue engineering.

show abstract

“…The incorporation of trophic factors as FGF-2, NGF, PDGF, and bGFG has also shown to enhance stemness of neural stem cells and favor its differentiation and proliferation when implanted with chitosan scaffolds or microspheres (Hoveizi et al, 2015;Skop et al, 2016;Moore et al, 2018). Recently, Skop and his collaborators designed a cell-scaffold strategy employing a radial glial neural precursor cell line that conditionally secreted insulinlike growth factor I.…”

Section: Chitosan-based Materials and Cell Therapy To The Cnsmentioning

confidence: 99%

Potential of Chitosan and Its Derivatives for Biomedical Applications in the Central Nervous System

Ojeda-Hernández

Canales-Aguirre

Matías‐Guiu

et al. 2020

Front. Bioeng. Biotechnol.

133

View full text Add to dashboard Cite

It is well known that the central nervous system (CNS) has a limited regenerative capacity and that many therapeutic molecules cannot cross the blood brain barrier (BBB). The use of biomaterials has emerged as an alternative to overcome these limitations. For many years, biomedical applications of chitosan have been studied due to its remarkable biological properties, biocompatibility, and high versatility. Moreover, the interest in this biomaterial for CNS biomedical implementation has increased because of its ability to cross the BBB, mucoadhesiveness, and hydrogel formation capacity. Several chitosanbased biomaterials have been applied with promising results as drug, cell and gene delivery vehicles. Moreover, their capacity to form porous scaffolds and to bear cells and biomolecules has offered a way to achieve neural regeneration. Therefore, this review aims to bring together recent works that highlight the potential of chitosan and its derivatives as adequate biomaterials for applications directed toward the CNS. First, an overview of chitosan and its derivatives is provided with an emphasis on the properties that favor different applications. Second, a compilation of works that employ chitosan-based biomaterials for drug delivery, gene therapy, tissue engineering, and regenerative medicine in the CNS is presented. Finally, the most interesting trends and future perspectives of chitosan and its derivatives applications in the CNS are shown.

show abstract

Tethered growth factors on biocompatible scaffolds improve stemness of cultured rat and human neural stem cells and growth of oligodendrocyte progenitors

Cited by 13 publications

References 17 publications

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

Chitosan Scaffolds as Microcarriers for Dynamic Culture of Human Neural Stem Cells

Molecular genetic analysis of neural stem cells after space flight and simulated microgravity on earth

Potential of Chitosan and Its Derivatives for Biomedical Applications in the Central Nervous System

Contact Info

Product

Resources

About