Tethered Lipid Membranes as a Nanoscale Arrangement towards Non-Invasive Analysis of Acute Pancreatitis

Abstract

Extracellular heat shock proteins (HSPs) mediate immunological functions and are involved in pathologies such as infection, stress, and cancer. Here, we demonstrated the dependence of an amount of HSP70 and HSP90 in serum vs. severity of acute pancreatitis (AP) on a cohort of 49 patients. Tethered bilayer lipid membranes (tBLMs) have been developed to investigate HSPs’ interactions with tBLMs that can be probed by electrochemical impedance spectroscopy (EIS). The results revealed that HSP70 and HSP90 interact via different mechanisms. HSP70 shows the damage of the membrane, while HSP90 increases the insulation properties of tBLM. These findings provide evidence that EIS offers a novel approach for the study of the changes in membrane integrity induced by HSPs proteins. Herein, we present an alternative electrochemical technique, without any immunoprobes, that allows for the monitoring of HSPs on nanoscaled tBLM arrangement in biologics samples such us human urine. This study demonstrates the great potential of tBLM to be used as a membrane based biosensor for novel, simple, and non-invasive label-free analytical system for the prediction of AP severity.

Damage associated molecular patterns and neutrophil extracellular traps in acute pancreatitis

“…In addition, low serum HSP70 levels are associated with poor prognosis in AP patients (Arriaga-Pizano et al, 2018). Budvytyte et al discussed these two findings above, as they found out the serum HSP90 levels increased linearly with increasing AP severity while the relationship was not linear in the case of HSP70 (Budvytyte et al, 2021). The mechanisms of extracellular HSP in AP are not yet conclusively elucidated, so further investigations are still required.…”