Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration

Lee, Jae Ho; Park, Jeong-Hui; Yun, Ye Rang; Jang, Jingon; Lee, Eun Jung; Chrzanowski, Wojciech; Wall, Ivan; Kim, Hae Won

doi:10.1039/c3tb00043e

Cited by 26 publications

(18 citation statements)

References 44 publications

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“…To overcome these limitations, recent approaches have shifted towards additive manufacturing. Some of the additive manufacturing approaches that can be used to engineer interface tissues include 3D printing, [133][134][135] stereolithography, air pressure aided deposition, [136,137] and robotic dispensing [138][139][140]. These freeform prototyping techniques face problems of bio-printability, which limit the use of printing cells with the scaffolds.…”

Section: Emerging Trends and Techniquesmentioning

confidence: 99%

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

et al. 2016

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Section: Emerging Trends and Techniquesmentioning

confidence: 99%

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

et al. 2016

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“…In fact, a variety of celladhesive proteins or peptides have been developed onto the surface of polymeric scaffolds (Zhang et al 2010;Lee et al 2013). Among the molecules, fibronectin (FN) has been a potent adhesive ligand that particularly stimulates the anchorage of mesenchymal stem cells (MSCs) (Lee et al 2013). Accelerating initial adhesion events of MSCs is considered to be a promising tool to improve the capacity of scaffolds targeting stem cell-based bone tissue engineering.…”

Section: Introductionmentioning

confidence: 99%

Fibronectin immobilization on to robotic-dispensed nanobioactive glass/polycaprolactone scaffolds for bone tissue engineering

et al. 2014

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Bioactive nanocomposite scaffolds with cell-adhesive surface have excellent bone regeneration capacities. Fibronectin (FN)-immobilized nanobioactive glass (nBG)/polycaprolactone (PCL) (FN-nBG/PCL) scaffolds with an open pore architecture were generated by a robotic-dispensing technique. The surface immobilization level of FN was significantly higher on the nBG/PCL scaffolds than on the PCL scaffolds, mainly due to the incorporated nBG that provided hydrophilic chemical-linking sites. FN-nBG/PCL scaffolds significantly improved cell responses, including initial anchorage and subsequent cell proliferation. Although further in-depth studies on cell differentiation and the in vivo animal responses are required, bioactive nanocomposite scaffolds with cell-favoring surface are considered to provide promising three-dimensional substrate for bone regeneration.

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“…11,12 For example, the scaffolds tethered with bone ECM proteins significantly induced stem cell differentiation and bone formation. [13][14][15] When the surface of scaffolds was tailored to be bone-bioactive, the calcium and phosphate ions could be substantially induced to form a bone-mineral-like phase that assists in protein adsorption and cellular anchorage. 13,16,17 Furthermore, when the matrix surface was engineered to have stiffer mechanical properties, the osteoprogenitor or stem cells were better driven to an osteogenic lineage through the mechanical signaling pathways.…”

Section: Introductionmentioning

confidence: 99%

Mesoporous Silica-Layered Biopolymer Hybrid Nanofibrous Scaffold: A Novel Nanobiomatrix Platform for Therapeutics Delivery and Bone Regeneration

Singh¹,

Jin²,

Mahapatra³

et al. 2015

ACS Appl. Mater. Interfaces

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Nanoscale scaffolds that characterize high bioactivity and the ability to deliver biomolecules provide a 3D microenvironment that controls and stimulates desired cellular responses and subsequent tissue reaction. Herein novel nanofibrous hybrid scaffolds of polycaprolactone shelled with mesoporous silica (PCL@MS) were developed. In this hybrid system, the silica shell provides an active biointerface, while the 3D nanoscale fibrous structure provides cell-stimulating matrix cues suitable for bone regeneration. The electrospun PCL nanofibers were coated with MS at controlled thicknesses via a sol-gel approach. The MS shell improved surface wettability and ionic reactions, involving substantial formation of bone-like mineral apatite in body-simulated medium. The MS-layered hybrid nanofibers showed a significant improvement in mechanical properties, in terms of both tensile strength and elastic modulus, as well as in nanomechanical surface behavior, which is favorable for hard tissue repair. Attachment, growth, and proliferation of rat mesenchymal stem cells were significantly improved on the hybrid scaffolds, and their osteogenic differentiation and subsequent mineralization were highly up-regulated by the hybrid scaffolds. Furthermore, the mesoporous surface of the hybrid scaffolds enabled the loading of a series of bioactive molecules, including small drugs and proteins at high levels. The release of these molecules was sustainable over a long-term period, indicating the capability of the hybrid scaffolds to deliver therapeutic molecules. Taken together, the multifunctional hybrid nanofibrous scaffolds are considered to be promising therapeutic platforms for stimulating stem cells and for the repair and regeneration of bone.

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Tethering bi-functional protein onto mineralized polymer scaffolds to regulate mesenchymal stem cell behaviors for bone regeneration

Cited by 26 publications

References 44 publications

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

Nanoengineered biomaterials for repair and regeneration of orthopedic tissue interfaces

Fibronectin immobilization on to robotic-dispensed nanobioactive glass/polycaprolactone scaffolds for bone tissue engineering

Mesoporous Silica-Layered Biopolymer Hybrid Nanofibrous Scaffold: A Novel Nanobiomatrix Platform for Therapeutics Delivery and Bone Regeneration

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