Tethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogels

Flora, Tatjana; Torre, Israel González de; Alonso, Matilde; Rodríguez-Cabello, J Carlos

doi:10.1007/s10856-019-6232-z

Cited by 48 publications

(30 citation statements)

References 49 publications

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“…30,31 Next to functionalization with adhesion peptides, combinations with growth factor-mimetic sequences additionally promote endothelialization. 32 However, approaches implementing peptide-mediated and affinity-based incorporation of heparin and cytokines in combination with short adhesion peptides have only been exploited in form of hydrogels so far. 33 In the present study, a mussel-derived surface coating combining cell-adhesive as well as heparin-binding and subsequent cytokine-delivering properties is proposed.…”

Section: Discussionmentioning

confidence: 99%

Multifunctional coatings combining bioactive peptides and affinity-based cytokine delivery for enhanced integration of degradable vascular grafts

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Multifunctional coatings combining bioactive peptides and affinity-based cytokine delivery for enhanced integration of degradable vascular grafts

et al. 2020

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“…Accordingly, there is much interest in functionalizing these materials with angiogenic factors (Du, Liu, et al, ; Liekens et al, ; Pensa et al, ). The VEGF‐derived QK peptide is a robust activator of neovascularization, and has therefore been a focus of many tissue regenerative strategies (Cai et al, ; Chan et al, ; Flora, de Torre, Alonso, & Rodriguez‐Cabello, ; Pensa et al, ; Prakash Parthiban, Rana, Jabbari, Benkirane‐Jessel, & Ramalingam, ). In multiple preclinical animal models, the QK peptide and rVEGF have been reported to induce a comparable degree of angiogenesis (Finetti et al, ; Santulli et al, ).…”

Section: Discussionmentioning

confidence: 99%

Sustained delivery of the angiogenic QK peptide through the use of polyglutamate domains to control peptide release from bone graft materials

Pensa

Curry

Reddy

et al. 2019

J Biomedical Materials Res

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Angiogenesis plays a pivotal role in tissue regeneration following bone‐grafting procedures; however, nonautogenous graft materials typically lack critical angiogenic growth factors. While much research has focused on modifying grafts with angiogenic factors, controlled delivery of these molecules remains a challenge. The current study describes a method for sustained delivery of an angiogenic peptide from hydroxyapatite (HA), a common alloplast material. Specifically, VEGF‐derived “QK” peptides were synthesized with polyglutamate domains containing varying numbers of glutamates. The rate of peptide release from HA inversely correlated with glutamate number, with diglutamate‐QK (E2‐QK) released first, followed by tetraglutamate‐QK (E4‐QK), and finally, heptaglutamate‐QK (E7‐QK). By coating HA with a mixture of these peptides, termed, PGM‐QK (polyglutamate‐modified mixture), sequential peptide release was achieved, enabling gradient QK delivery. To evaluate bioactivity, HA disks were coated with PGM‐QK and then placed in fresh media for 6 days. Media containing the released peptides was collected at varying time intervals and placed on human umbilical vein endothelial cells (HUVECs). Cells were evaluated for activation of angiogenic signaling pathways (ERK and Akt) and cell migration. Results showed that QK peptides were continuously released over the 6‐day interval, and maintained their capacity to activate HUVECs. These findings point to a new approach for gradient delivery of an angiogenic stimulus.

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“…John et al (2019) fabricated a GelMA nanofiber microsphere coated with VEGF-mimic peptides, and showed the formation of vascular tubes by HUVEC. Similarly, Flora et al (2019) demonstrated that elastin-like recombinamer (ELR) tethered with VEGFmimic peptides not only improved vascularization in vitro, but also in vivo. In fact, ELR injected into mice enhanced the recruitment and proliferation of ECs, and the number of capillaries (Flora et al, 2019).…”

Section: Promotion Of Cell Adhesionmentioning

confidence: 97%

Angiogenesis in Tissue Engineering: As Nature Intended?

Mastrullo

Cathery

Velliou

et al. 2020

Front. Bioeng. Biotechnol.

138

109

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Despite the steady increase in the number of studies focusing on the development of tissue engineered constructs, solutions delivered to the clinic are still limited. Specifically, the lack of mature and functional vasculature greatly limits the size and complexity of vascular scaffold models. If tissue engineering aims to replace large portions of tissue with the intention of repairing significant defects, a more thorough understanding of the mechanisms and players regulating the angiogenic process is required in the field. This review will present the current material and technological advancements addressing the imperfect formation of mature blood vessels within tissue engineered structures.

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Tethering QK peptide to enhance angiogenesis in elastin-like recombinamer (ELR) hydrogels

Abstract: 6232-z ". "Esta es una preimpresión de un artículo publicado en Journal of Materials Science Materials in Medicine. La versión final autenticada está disponible en línea en:

Cited by 48 publications

References 49 publications

Multifunctional coatings combining bioactive peptides and affinity-based cytokine delivery for enhanced integration of degradable vascular grafts

Multifunctional coatings combining bioactive peptides and affinity-based cytokine delivery for enhanced integration of degradable vascular grafts

Sustained delivery of the angiogenic QK peptide through the use of polyglutamate domains to control peptide release from bone graft materials

Angiogenesis in Tissue Engineering: As Nature Intended?

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