TetR-Type Regulator Lp_2642 Positively Regulates Plantaricin EF Production Based on Genome-Wide Transcriptome Sequencing of <i>Lactiplantibacillus plantarum</i> 163

Zhao, Deyin; Wang, Qian; Meng, Fanqiang; Lu, Fengxia; Bie, Xiaomei; Lu, Zhaoxin; Lu, Yingjian

doi:10.1021/acs.jafc.2c00206

Cited by 5 publications

(2 citation statements)

References 51 publications

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“…The transcriptomic and proteomic data of L. plantarum 163 under glucose and maltose conditions were determined in a previous study. , The transcriptomic data were obtained from the NCBI database (SRA: SRR12831476), and the proteomic data were obtained from the National Protein Science Center (Beijing, China) (PXD022981). Gene and protein changes in glycolysis, pentose phosphate pathway, tricarboxylic acid cycle, and purine metabolism were analyzed.…”

Section: Methodsmentioning

confidence: 99%

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Meng

Lyu

Chen

et al. 2023

J. Agric. Food Chem.

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Carbon sources alter the synthesis of exopolysaccharides (EPS) in Lactiplantibacillus plantarum. Maltose increased the EPS production of L. plantarum 163 6.5-fold. Subsequently, EPS production, transcriptome, and proteome were analyzed using glucose or maltose to further clarify the regulatory mechanism. A cAMP receptor protein (UniProtKB: F9UNI5) has been identified to control EPS synthesis in the presence of cAMP by binding to the EPS synthesis promoter Pcps4A‑J. Overexpression of the cAMP synthesis gene cyaA increased cAMP content and EPS production 4.5- and 2.2-fold, respectively. Furthermore, yogurt produced with L. plantarum 163-cyaA had a similar viscosity to that of commercial Greek yogurt; it had 20 and 83.7% greater viscosity than that produced with L. plantarum 163 with maltose and glucose, respectively. These findings indicated that L. plantarum 163-cyaA has potential applications in the production of functional fermented dairy products.

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Section: Methodsmentioning

confidence: 99%

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Meng

Lyu

Chen

et al. 2023

J. Agric. Food Chem.

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“…This study identified unique genes and metabolic pathways in L. plantarum K25 that may have potential applications in the fields of probiotics and biotechnology. However, there are still knowledge gaps in our understanding of the genome of L. plantarum K25, particularly in the role of certain genes and the mechanisms underlying its probiotic properties (Zhao et al, 2022). Further research is needed to fill these gaps and fully unlock the potential of Lactiplantibacillus K25 for improving human health and advancing biotechnological research.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomics of food-derived probiotic Lactiplantibacillus plantarum K25 reveals its hidden potential, compactness, and efficiency

et al. 2023

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This study aimed to investigate the intricate genetic makeup of the Lactiplantibacillus plantarum K25 strain by conducting a comprehensive analysis of comparative genomics. The results of our study demonstrate that the genome exhibits a high-level efficiency and compactness, comprising a total of 3,199 genes that encode proteins and a GC content of 43.38%. The present study elucidates the evolutionary lineage of Lactiplantibacillus plantarum strains through an analysis of the degree of gene order conservation and synteny across a range of strains, thereby underscoring their closely interrelated evolutionary trajectories. The identification of various genetic components in the K25 strain, such as bacteriocin gene clusters and prophage regions, highlights its potential utility in diverse domains, such as biotechnology and medicine. The distinctive genetic elements possess the potential to unveil innovative therapeutic and biotechnological remedies in future. This study provides a comprehensive analysis of the L. plantarum K25 strain, revealing its remarkable genomic potential and presenting novel prospects for utilizing its unique genetic features in diverse scientific fields. The present study contributes to the existing literature on Lactiplantibacillus plantarum and sets the stage for prospective investigations and practical implementations that leverage the exceptional genetic characteristics of this adap organism.

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Regulatory Effect of Transcriptional Regulator TetR on the Synthesis of Conjugated Linoleic Acid in Lactiplantibacillus plantarum AR195

Liu,

Dou,

Liu

et al. 2024

J. Agric. Food Chem.

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Conjugated linoleic acid (CLA) possesses anticancer, anti-inflammatory, and antiobesity properties, making it a significant research focus. In this study, we identified TetR, a TetR/AcrR family transcriptional regulator encoded by AR1031, as the transcriptional regulator of CLA synthesis in Lactiplantibacillus plantarum AR195. TetR binds to the promoter regions of the CLA synthesis genes, including the cla operon and cla-hy, thereby enhancing CLA biosynthesis. Knockout of tetR led to the downregulation of cla-hy by 68%, cla-dh by 86%, and cla-dc by 33%, resulting in reduced CLA yield. Further bioinformatic analysis and segmented electrophoretic mobility shift assay (EMSA) experiments revealed that TetR recognizes a conserved sequence (5′-TGTAGATTG-n 4 -CTTCA-3′) and binds to multiple sites within the regulatory region of the cla operon and cla-hy, thus promoting CLA biosynthesis. Overexpression of tetR increased the CLA yield by 7%. These findings provide precise insights into the regulation of CLA biosynthesis and suggest strategies for enhancing CLA production.

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TetR-Type Regulator Lp_2642 Positively Regulates Plantaricin EF Production Based on Genome-Wide Transcriptome Sequencing of Lactiplantibacillus plantarum 163

Abstract: Whole-genome and transcriptome sequences of Lactiplantibacillus plantarum 163 are provided. There was one circular chromosome and four circular plasmids,

Cited by 5 publications

References 51 publications

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Comparative genomics of food-derived probiotic Lactiplantibacillus plantarum K25 reveals its hidden potential, compactness, and efficiency

Regulatory Effect of Transcriptional Regulator TetR on the Synthesis of Conjugated Linoleic Acid in Lactiplantibacillus plantarum AR195

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