Tetrachlorvinphos metabolism in laying hens

Abstract

Tetrachlorvinphos metabolism was studied, with the aid of a uC-labeled compound, in laying hens fed 50 ppm of the insecticide. Approximately 71% of the radioactivity was eliminated in 24 h in excreta. After the final dose, an additional 1.3 and 2.6% of total UC was excreted during the next 3 and 7 days. Eggs laid within 24 h of the treatment contained radioactivity. 14C was also detected in tissues and organs at an insecticide equivalent in the ppm range in kidney, liver, and abdominal fat but in the ppb range elsewhere. After dosing was discontinued, the 14C content of excreta, eggs, tissues, and organs gradually decreased. Compounds identified in excreta were desmethyl tetrachlorvinphos (25%), 2,4,5-trichloromandelic acid (30.5%), and tetrachlorvinphos (1.0%). During the treatment period, all tissues examined contained small amounts of the insecticide, but only traces were detected in tissues and organs from hens killed 7 days after the last dose.

Pharmacokinetics of veterinary drugs in laying hens and residues in eggs: a review of the literature

“…, 1969; Yadava & Shaw, 1970; Sherman & Herrick, 1971; Pitois et al. , 1973b; Gupta & Paul, 1977; Akhtar & Foster, 1981). While in chickens the majority of an organophosphate dose is generally eliminated in a few days, detectable residues can persist in tissues and eggs for several weeks (March et al.…”

Fate of trichlorfon in buffer and soluble fraction (105000 g) from cow and chicken liver homogenates

“…Silylating agents Tri-Sil and Tri-Sil Concentrate were purchased from Chromatographic Specialties, Ltd., Brockville, Ontario, Canada. Enzyme Preparation. from livers of chicken and cow were prepared in ice-cold 0.134 M phosphate buffer (pH 7.4) in a glass-Teflon homogenizer as described earlier (Akhtar and Foster, 1977).…”

“…Incubation and Degradation. In general, an enzymatic reaction can be terminated by the addition of acetone (Akhtar and Foster, 1977). For termination of both the " Samples were added to buffer and shaken for 30 s, and 2 mL of 0.8% HC1 was added.…”