Tetracycline and ciprofloxacin multiresidues in beef and chicken meat samples using indirect competitive ELISA

Baghani, Akram; Mesdaghinia, Alireza; Rafieiyan, Mahsa; Dallal, M M Soltan; Douraghi, Masoumeh

doi:10.1080/15321819.2019.1597735

Cited by 46 publications

(25 citation statements)

References 30 publications

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“…The consumption of contaminated food is associated to allergic reactions, anaphylactic shock and alteration of the immune system, but bacterial resistance is the greatest concern with regard to the use of FQs in animal husbandry [5,6]. Moreover, FQs for exclusive use in humans as norfloxacin (NOR) and ciprofloxacin (CIPRO) have been detected in animal tissues as well as sarafloxacin (SARA) that is used in poultry [7][8][9][10][11]. The problem of FQs residues in edible products is linked to ineffective government policies and poor implementation of good agricultural practices in developing countries, which will be responsible for 76% of the output growth expected in 2027.…”

Section: Introductionmentioning

confidence: 99%

Residues of Fluoroquinolone Antibiotics Induce Carbonylation and Reduce In Vitro Digestion of Sarcoplasmic and Myofibrillar Beef Proteins

Lázaro

Méndez-Cuadro

Rodríguez-Cavallo

2020

Foods

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Although the impact of oxidation on human health has been of growing interest, the oxidation of proteins, major component of meat, has received little attention. This paper describes the in vitro effect of five fluoroquinolones (FQs) on carbonylation of sarcoplasmic and myofibrillar proteins of beef when found at concentrations close to the maximum residue limit (MRL). Samples were treated individually with the FQs, determining in each protein fraction the carbonyl index, protein content and oxidized proteins identification, using 2,4-dinitrophenyhydrazine (DNPH) alkaline assay, Western blot and Bradford methods, and mass spectrometry, respectively. Besides, the in vitro effect of these residues on gastric and duodenal digestion of proteins was evaluated. The carbonylation induced by FQs affected both protein fractions being significant with respect to the blank in 73.3% of cases. This damage was correlated with loss of solubility and digestibility, with sarcoplasmic proteins the most affected. Danofloxacin and enrofloxacin were the FQs with greatest oxidant effects, especially affecting glycolysis and glycogen proteins. Our results suggest that these residues induce irreversible oxidative damage on the main beef proteins and could affect their nutritional value.

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Section: Introductionmentioning

confidence: 99%

Residues of Fluoroquinolone Antibiotics Induce Carbonylation and Reduce In Vitro Digestion of Sarcoplasmic and Myofibrillar Beef Proteins

Lázaro

Méndez-Cuadro

Rodríguez-Cavallo

2020

Foods

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“…Due to its extension, only the materials used for the extraction of antibiotics in meat samples will be next explained. Molecularly imprinted polymers (MIPs) have been commonly used for this purpose (Feng, Wang, Yang, Liu, & Wang, 2016; Ma et al., 2020; Savoy et al., 2018; Song et al, 2017; Song et al., 2019; B. Yang, 2019). Their use is very attractive for the extraction of antibiotics because they are prepared from a polymerization mixture containing a template molecule that allows the creation of devoted cavities with specific interactions along the polymerization.…”

Section: Sample Pretreatment Techniquesmentioning

confidence: 99%

Determination of antibiotics in meat samples using analytical methodologies: A review

Moga

Vergara-Barberán

Lerma‐García

et al. 2021

Comp Rev Food Sci Food Safe

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Antibiotics are widely used to prevent or treat some diseases in human and veterinary medicine and also as animal growth promoters. The presence of these compounds in foods derived from food‐producing animals can be a risk for human health. Consequently, regulatory agencies have set maximum residue limits for antibiotics in food samples. Therefore, the development of novel methodologies for its determination in food samples is required. Specifically, the analysis and quantification of these substances in meat tissues is a challenge for the analytical chemistry research community. This is due to the complexity of the matrix and the low detection limits required by the regulatory agencies. In this sense, a comprehensive review on the development of new sample preparation treatments involving extraction, cleanup, and enrichment steps of antibiotics in meat samples in combination with sensitive and sophisticated determination techniques that have been carry out in the last years is necessary. Therefore, the aim of this work is to summarize the published methodologies for the determination of antibiotics from 2016 until the beginning of the second semester of 2020. The first part of this review includes an introduction about antibiotic families, followed by sample preparation and determination techniques applied to the different families. Finally, a detailed discussion of the current trends and the future possible perspectives in this field are also included.

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“…However, these systems are limited by complicated sample pre-treatments and require highly trained technicians and expensive equipment [8]. On the other hand, rapid methods such as immunoassays [9], spectroscopic analyses [10], and electrochemical techniques [11] provide relatively convenient and highly sensitive strategies for the determination of pesticides and veterinary drugs. Although the accuracy and precision of rapid methods are not as good as those of instrumental techniques, these methods can be used complementarily to instrumental methods, particularly as pre-screening methods for detection in large-scale samples.…”

Section: Introductionmentioning

confidence: 99%

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

et al. 2020

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The excessive use or abuse of pesticides and veterinary drugs leads to residues in food, which can threaten human health. Therefore, there is an extremely urgent need for multi-analyte analysis techniques for the detection of pesticide and veterinary drug residues, which can be applied as screening techniques for food safety monitoring and detection. Recent developments related to rapid multi-residue detection methods for pesticide and veterinary drug residues are reviewed herein. Methods based on different recognition elements or the inherent characteristics of pesticides and veterinary drugs are described in detail. The preparation and application of three broadly specific recognition elements—antibodies, aptamers, and molecular imprinted polymers—are summarized. Furthermore, enzymatic inhibition-based sensors, near-infrared spectroscopy, and SERS spectroscopy based on the inherent characteristics are also discussed. The aim of this review is to provide a useful reference for the further development of rapid multi-analyte analysis of pesticide and veterinary drug residues.

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Tetracycline and ciprofloxacin multiresidues in beef and chicken meat samples using indirect competitive ELISA

Cited by 46 publications

References 30 publications

Residues of Fluoroquinolone Antibiotics Induce Carbonylation and Reduce In Vitro Digestion of Sarcoplasmic and Myofibrillar Beef Proteins

Residues of Fluoroquinolone Antibiotics Induce Carbonylation and Reduce In Vitro Digestion of Sarcoplasmic and Myofibrillar Beef Proteins

Determination of antibiotics in meat samples using analytical methodologies: A review

Rapid Multi-Residue Detection Methods for Pesticides and Veterinary Drugs

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