Tetracycline-Containing MCM-41 Mesoporous Silica Nanoparticles for the Treatment of Escherichia coli

Abstract

Tetracycline (TC) is a well-known broad spectrum antibiotic, which is effective against many Gram positive and Gram negative bacteria. Controlled release nanoparticle formulations of TC have been reported, and could be beneficial for application in the treatment of periodontitis and dental bone infections. Furthermore, TC-controlled transcriptional regulation systems (Tet-on and Tet-off) are useful for controlling transgene expression in vitro and in vivo for biomedical research purposes; controlled TC release systems could be useful here, as well. Mesoporous silica nanomaterials (MSNs) are widely studied for drug delivery applications; Mobile crystalline material 41 (MCM-41), a type of MSN, has a mesoporous structure with pores forming channels in a hexagonal fashion. We prepared 41˘4 and 406˘55 nm MCM-41 mesoporous silica nanoparticles with loaded TC for controlled drug release; TC content in the TC-MCM-41 nanoparticles was 18.7% and 17.7% w/w, respectively. Release of TC from TC-MCM-41 nanoparticles was then measured in phosphate-buffered saline (PBS), pH 7.2, at 37˝C over a period of 5 h. Most antibiotic was released from both over this observation period; however, the majority of TC was released over the first hour. Efficacy of the TC-MCM-41 nanoparticles was then shown to be superior to free TC against Escherichia coli (E. coli) in culture over a 24 h period, while blank nanoparticles had no effect.

Characterization, drug loading and antibacterial activity of nanohydroxyapatite/polycaprolactone (nHA/PCL) electrospun membrane

“…The samples were then immersed into 10 mL phosphate buffer solution (PBS) at 37°C. After each time interval, 1 mL of PBS was taken from the sample tubes and then the absorbance was measured using a UV spectroscopy at a wavelength of 275 nm (Koneru et al 2015). UV-visible spectroscopy measurements were performed for known concentrations of TCH at the absorbance of 275 nm and a standard curve was constructed (Koneru et al 2015).…”

Effects of Chlorhexidine-Encapsulated Mesoporous Silica Nanoparticles on the Anti-Biofilm and Mechanical Properties of Glass Ionomer Cement

“…Mesoporous silica nanoparticles (MSN), labeled with stable framework, high specific surface area, tailored mesoporous structure, and favorable biocompatibility, are an ideal host for multifarious molecules [ 22 ]. MSN was first proposed by Yanagisawa et al in 1990 [ 23 ], and has been widely used as a drug delivery system for antibiotics, anti-inflammatory agent, and anti-cancer drugs since these particles can function as reservoirs for sustained drug release [ 17 , 24 , 25 , 26 ]. Simply mixing additives would alter the mechanical properties of GIC [ 4 , 13 , 18 , 19 , 20 , 21 ]; as such, we speculate that the good dispersibility of MSN [ 27 ] might be favorable for encapsulating and continuously releasing CHX without reducing the mechanical performance of GIC.…”

Effects of Particle Hydrophobicity, Surface Charge, Media pH Value and Complexation with Human Serum Albumin on Drug Release Behavior of Mitoxantrone-Loaded Pullulan Nanoparticles

“…With fast drug metabolism, the drug reaches peak concentrations quickly, which usually leads to severe drug toxicity, and the peak-valley reaction is inefficient or invalid. To avoid these problems, a slow-release drug strategy has been developed to preserve the drug-efficient concentration in the blood by a sustained release effect and has become a major research field [ 1 , 2 ].…”