Tetrahedral framework nucleic acid carrying angiogenic peptide prevents bisphosphonate-related osteonecrosis of the jaw by promoting angiogenesis

Abstract

The significant clinical feature of bisphosphonate-related osteonecrosis of the jaw (BRONJ) is the exposure of the necrotic jaw. Other clinical manifestations include jaw pain, swelling, abscess, and skin fistula, which seriously affect the patients’ life, and there is no radical cure. Thus, new methods need to be found to prevent the occurrence of BRONJ. Here, a novel nanoparticle, tFNA-KLT, was successfully synthesized by us, in which the nanoparticle tetrahedral framework nucleic acid (tFNA) was used for carrying angiogenic peptide, KLT, and then further enhanced angiogenesis. TFNA-KLT possessed the same characteristics as tFNA, such as simple synthesis, stable structure, and good biocompatibility. Meanwhile, tFNA enhanced the stability of KLT and carried more KLT to interact with endothelial cells. First, it was confirmed that tFNA-KLT had the superior angiogenic ability to tFNA and KLT both in vitro and in vivo. Then we apply tFNA-KLT to the prevention of BRONJ. The results showed that tFNA-KLT can effectively prevent the occurrence of BRONJ by accelerating angiogenesis. In summary, the prepared novel nanoparticle, tFNA-KLT, was firstly synthesized by us. It was also firstly confirmed by us that tFNA-KLT significantly enhanced angiogenesis and can effectively prevent the occurrence of BRONJ by accelerating angiogenesis, thus providing a new avenue for the prevention of BRONJ and a new choice for therapeutic angiogenesis.

Albumin-Coated Framework Nucleic Acids as Bionic Delivery System for Triple-Negative Breast Cancer Therapy

“…The Annexin V-APC/7-AAD Apoptosis Detection Kit tested cell apoptosis by an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific). 29,33 To observe the expression and localization of apoptosis-related proteins, cells in confocal dishes were treated with BSA@T and BSA@ DAT for 48 h. Then, all samples were fixed in paraformaldehyde solution (4% w/v), treated with 0.5% Triton X-100, and blocked with goat serum. Primary antibodies (anti-vleaved caspase 3, anti-Bax, and anti-Bcl2) were incubated with the samples overnight.…”

“…Subsequently, samples were incubated with DAT and BSA@DAT for 24, and 48 h and harvested by trypsinization (0.25% Trypsin, without EDTA, (Hyclone). The Annexin V-APC/7-AAD Apoptosis Detection Kit tested cell apoptosis by an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific). , …”

Improved Tumor Infiltration and Immunomodulation for Tumor Therapy: A Pathway Based on Tetrahedral Framework Nucleic Acids Coupled Bacterial Nanocells

“…AT5 was constructed in accordance with a well-established technical route outlined in a previous work. 31 Four specific ssDNAs were designed for AT5 (S1, S2-AS1411, S3-5-FU, and S4) and T (S1, S2, S3, and S4). The sequences were detailed in Table S1.…”

Self-Assembled Immunostimulatory Tetrahedral Framework Nucleic Acid Vehicles for Tumor Chemo-immunotherapy

“…Remarkably, as a kind of framework nucleic acid synthesized from oligonucleotides by Watson−Crick pairing, tFNA, exhibited great potential in drug delivery for its outstanding intrinsic biocompatibility 8 superior membrane permeability. 9−11 Its extensive biological application in drug or gene-targeted delivery 12 and diagnosis has 13,14 has been widely reported. 10,15 As a result, the application of tFNA in passive tumor-targeted delivery via EPR was promising because of its special framework morphology and desirable size.…”

“…This phenomenon is known as the enhanced permeability and retention (EPR) effect. , Although the development of drug-loaded nanoparticles has been evolving for decades, they have not yet been widely approved for clinical use due to biosafety concerns. Remarkably, as a kind of framework nucleic acid synthesized from oligonucleotides by Watson–Crick pairing, tFNA, exhibited great potential in drug delivery for its outstanding intrinsic biocompatibility and superior membrane permeability. − Its extensive biological application in drug or gene-targeted delivery and diagnosis has , has been widely reported. , As a result, the application of tFNA in passive tumor-targeted delivery via EPR was promising because of its special framework morphology and desirable size. − …”