Tetrahedral framework nucleic acids prevent retina ischemia-reperfusion injury from oxidative stress <i>via</i> activating the Akt/Nrf2 pathway

Xin, Qin; Ni, Li; Zhang, Mei; Lin, Shiyu; Zhu, Junyao; Xiao, Dexuan; Cui, Weitong; Zhang, Tianyi; Lin, Yunfeng; Cai, Xiaoxiao

doi:10.1039/c9nr07171g

Cited by 67 publications

(55 citation statements)

References 43 publications

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“… 23 – 25 Moreover, cellular uptake is the key to the various effects of tFNAs on wound healing. 26 , 28 Therefore, after we successfully prepared tFNA nanostructures of an appropriate size and with a stable zeta potential, we demonstrated that tFNAs could be taken up in abundance by these two types of cells.…”

Section: Discussionmentioning

confidence: 92%

“… 27 – 30 In addition, tFNAs may influence different signaling pathways, such as the Wnt pathway and the Nrf2 pathway. 27 , 28 However, to the best of our knowledge, there have been few reports regarding nucleic-acid nanophase materials that directly affect the skin wound healing process without delivery assistance. In this study, we focused on evaluating the effects of tFNAs on keratinocytes (HaCaT cell line) and fibroblasts(HSF cell line) in vitro and in rat wound models in vivo.…”

Section: Introductionmentioning

confidence: 99%

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Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

Zhu

Zhang

Gao

et al. 2020

Sig Transduct Target Ther

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While the skin is considered the first line of defense in the human body, there are some vulnerabilities that render it susceptible to certain threats, which is an issue that is recognized by both patients and doctors. Cutaneous wound healing is a series of complex processes that involve many types of cells, such as fibroblasts and keratinocytes. This study showed that tetrahedral framework nucleic acids (tFNAs), a type of self-assembled nucleic-acid material, have the ability to promote keratinocyte(HaCaT cell line) and fibroblast(HSF cell line) proliferation and migration in vitro. In addition, tFNAs increased the secretion of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in HSF cells and reduced the production of tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) in HaCaT cells by activating the AKT-signaling pathway. During in vivo experiments, tFNA treatments accelerated the healing process in skin wounds and decreased the development of scars, compared with the control treatment that did not use tFNAs. This is the first study to demonstrate that nanophase materials with the biological features of nucleic acids accelerate the healing of cutaneous wounds and reduce scarring, which indicates the potential application of tFNAs in skin tissue regeneration.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

Zhu

Zhang

Gao

et al. 2020

Sig Transduct Target Ther

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“…[ 20–24 ] In our early exploration in the prevention and treatment of various eye diseases, tFNAs have been proved to act a positive part as drugs in repairing corneal injury and the anti‐oxidative stress of RGCs. [ 25,26 ] Furthermore, our previous studies have shown that tFNAs can exert neuroprotective influence in the Alzheimer's disease in vitro model. [ 27 ] Hence, in this work, tFNAs were selected as the carrier for miR‐22‐3p to synthesize a complex of tFNAs and miRNAs (tFNAs‐miR22).…”

Section: Introductionmentioning

confidence: 99%

The Neuroprotective Effect of MicroRNA‐22‐3p Modified Tetrahedral Framework Nucleic Acids on Damaged Retinal Neurons Via TrkB/BDNF Signaling Pathway

Xiao

Yan

et al. 2021

Adv Funct Materials

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Glaucoma is the world's leading irreversible cause of blindness. It is characterized by progressive retinal ganglion cells (RGCs) loss and axon damage, and there is no effective prevention or treatment so far. In this study, tetrahedral frame nucleic acids (tFNAs) with various biological functions are selected as the carrier, and microRNA‐22‐3p (miR‐22) are modified by attaching them onto tFNAs to synthesize a novel DNA nanocomplex (tFNAs‐miR22). Relevant results show that tFNAs can efficiently transfer miR‐22 into damaged retinal neurons to impose a neuroprotective effect on glaucoma. More interestingly, a certain synergism between tFNAs and miR‐22 is shown. tFNAs‐miR22 can selectively activate tyrosine kinase receptor B (TrkB) and regulate the TrkB‐brain‐derived growth factor (BDNF) signaling pathway to restore the expression of BDNF on the models of N‐methyl‐D‐aspartate (NMDA)‐induced glaucoma in vivo and in vitro, thus exerting the neuroprotective effect on retinal neurons. This study shows the successfully establishment of a simple but effective delivery system of microRNAs associated with glaucoma treatment, which may be a promising neuroprotective agent for future treatment of this optic neurodegenerative disease.

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“…The search for new biologically active molecules for the prevention and correction of retinal ischemia-reperfusion is one of the tasks of current ocular pharmacology [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Retinoprotective Effect of 2-Ethyl-3-hydroxy-6-methylpyridine Nicotinate

Peresypkina

Pazhinsky

Даниленко

et al. 2020

Biology

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An important task of pharmacology is to find effective agents to improve retinal microcirculation and resistance to ischemia. The purpose of the study is to pharmacologically evaluate the retinoprotective effect of 2-ethyl-3-hydroxy-6-methylpyridine nicotinate in a rat model of retinal ischemia–reperfusion. A retinal ischemia–reperfusion model was used, in which an increase in intraocular pressure (IOP) to 110 mmHg was carried out within 30 min. The retinoprotective effect of 2-ethyl-3-hydroxy-6-methylpyridine nicotinate at a dose of 3.8 mg/kg, in comparison with nicotinic acid at a dose of 2 mg/kg and emoxipine at a dose of 2 mg/kg, was estimated by the changes in the eye fundus during ophthalmoscopy, the retinal microcirculation level with laser Doppler flowmetry (LDF), and electroretinography (ERG) after 72 h of reperfusion. The use of 2-ethyl-3-hydroxy-6-methylpyridine nicotinate prevented the development of ischemic injuries in the fundus and led to an increase in the retinal microcirculation level to 747 (median) (lower and upper quartiles: 693;760) perfusion units (p = 0.0002) in comparison with the group that underwent no treatment. In the group with the studied substance, the b-wave amplitude increased significantly (p = 0.0022), and the b/a coefficient increased reliably (p = 0.0002) in comparison with the group with no treatment. Thus, 2-ethyl-3-hydroxy-6-methylpyridine nicotinate has established itself as a potential retinoprotector.

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Tetrahedral framework nucleic acids prevent retina ischemia-reperfusion injury from oxidative stress via activating the Akt/Nrf2 pathway

Abstract: Retinal ischemia-reperfusion (I/R) injuries are involved in the universal pathological processes of many ophthalmic diseases, including glaucoma, diabetic retinopathy, and retinal arterial occlusion.

Cited by 67 publications

References 43 publications

Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

Tetrahedral framework nucleic acids promote scarless healing of cutaneous wounds via the AKT-signaling pathway

The Neuroprotective Effect of MicroRNA‐22‐3p Modified Tetrahedral Framework Nucleic Acids on Damaged Retinal Neurons Via TrkB/BDNF Signaling Pathway

Retinoprotective Effect of 2-Ethyl-3-hydroxy-6-methylpyridine Nicotinate

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