Tetrahydrobiopterin in antenatal brain hypoxia-ischemia-induced motor impairments and cerebral palsy

Vásquez‐Vivar, Jeannette; Shi, Zhijun; Luo, Kui; Thirugnanam, Karthikeyan; Tan, Sidhartha

doi:10.1016/j.redox.2017.08.002

Cited by 34 publications

(18 citation statements)

References 72 publications

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“…Cerebral ischemia markedly increases morbidity and mortality 34 , 35 . Therefore, the discovery of novel drugs for the treatment of ischemic stroke is critically needed.…”

Section: Discussionmentioning

confidence: 99%

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1

Xiao

Zhou

et al. 2018

Cell Death Dis

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The subcellular location of annexin A1 (ANXA1) determines the ultimate fate of neurons after ischemic stroke. ANXA1 nuclear translocation is involved in neuronal apoptosis after cerebral ischemia, and extracellular ANXA1 is also associated with regulation of inflammatory responses. As the factors and mechanism that influence ANXA1 subcellular translocation remain unclear, studies aiming to determine and clarify the role of ANXA1 as a cell fate ‘regulator’ within cells are critically needed. In this study, we found that intracerebroventricular injection of the recombinant adenovirus vector Ad-S100A11 (carrying S100A11) strongly improved cognitive function and induced robust neuroprotective effects after ischemic stroke in vivo. Furthermore, upregulation of S100A11 protected against neuronal apoptosis induced by oxygen-glucose deprivation and reoxygenation (OGD/R) in vitro. Surprisingly, S100A11 overexpression markedly decreased ANXA1 nuclear translocation and subsequently alleviated OGD/R-induced neuronal apoptosis. Notably, S100A11 exerted its neuroprotective effect by directly binding ANXA1. Importantly, S100A11 directly interacted with ANXA1 through the nuclear translocation signal (NTS) of ANXA1, which is essential for ANXA1 to import into the nucleus. Consistent with our previous studies, ANXA1 nuclear translocation after OGD/R promoted p53 transcriptional activity, induced mRNA expression of the pro-apoptotic Bid gene, and activated the caspase-3 apoptotic pathway, which was almost completely reversed by S100A11 overexpression. Thus, S100A11 protects against cell apoptosis by inhibiting OGD/R-induced ANXA1 nuclear translocation. This study provides a novel mechanism whereby S100A11 protects against neuronal cells apoptosis, suggesting the potential for a previously unidentified treatment strategy in minimizing apoptosis after ischemic stroke.

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“…Cerebral ischemia markedly increases morbidity and mortality 34 , 35 . Therefore, the discovery of novel drugs for the treatment of ischemic stroke is critically needed.…”

Section: Discussionmentioning

confidence: 99%

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1

Xiao

Zhou

et al. 2018

Cell Death Dis

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“…The initial attempts to measure BH 4 levels in diabetic rats and to confirm the reported BH 4 decrease failed because of co-elution of BH 4 with ascorbate, a known problem, especially since tissues such as the brain are very rich in ascorbate [32,67]. Upon further modification of the HPLC method, we were able to fully separate BH 4 and ascorbate peaks (protocol B).…”

Section: Discussionmentioning

confidence: 94%

Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection—Protocol Optimization and Pitfalls

Oelze

Kuntic

et al. 2022

Antioxidants

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Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determination of BH4 in tissue samples. We first determined the optimal storage and work-up conditions for authentic BH4 and its oxidation product dihydrobiopterin (BH2) under various conditions (pH, temperature, presence of antioxidants, metal chelators, and storage time). We then applied optimized protocols for detection of BH4 in tissues of septic (induced by lipopolysaccharide [LPS]) rats. BH4 standards in HCl are stabilized by addition of 1,4-dithioerythritol (DTE) and diethylenetriaminepentaacetic acid (DTPA), while HCl was sufficient for BH2 standard stabilization. Overnight storage of BH4 standard solutions at room temperature in HCl without antioxidants caused complete loss of BH4 and the formation of BH2. We further optimized the protocol to separate ascorbate and the BH4 tissue sample and found a significant increase in BH4 in the heart and kidney as well as higher BH4 levels by trend in the brain of septic rats compared to control rats. These findings correspond to reports on augmented nitric oxide and BH4 levels in both animals and patients with septic shock.

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“…Moreover, a 7 Computational and Mathematical Methods in Medicine relatively small number of studies were included, and their quality was not high. In certain severe clinical situations [40][41][42][43][44][45][46], the effectiveness and safety of ESPB still need to be evaluated.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Postoperative Analgesia by Erector Spinal Plane Block after Lumbar Surgery: A Systematic Review and Meta-analysis of Randomized Controlled Trials

Xiao

Zhu

Wang

et al. 2022

Computational and Mathematical Methods in Medicine

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Background. In recent years, erector spinae plane block (ESPB) has been increasingly used as a new regional block technique for postoperative analgesia; however, little is known on its benefits. Therefore, we performed a systematic review and meta-analysis to investigate the efficacy and safety of ESPB in lumbar spine surgery. Methods. Databases including PubMed, Embase, Cochrane Library, and Web of Science were systematically searched for randomized controlled trials (RCTs) comparing ESPB with no block in lumbar spine surgery until September 30, 2021. The primary outcome was opioid consumption after surgery. The Cochrane Collaboration’s tool for assessing the risk of bias was used to evaluate the quality of included studies. Results. Fifteen RCTs involving 980 patients were included in the study. Opioid consumption 24 hours after surgery was significantly lower in the ESPB group standardized mean difference ( SMD = − 2.27 , 95% confidence interval (95% CI) (-3.21, -1.32); p < 0.01 ). ESPB reduced pain scores at rest and on movement within 48 hours after surgery and the incidence of the postoperative rescue analgesia ( RR = 0.32 , 95% CI (0.31, 0.80); p = 0.02 ), while it significantly prolonged time to first rescue analgesia ( SMD = 4.87 , 95% CI (2.84, 6.90); p < 0.01 ). Moreover, significantly better patient satisfaction was associated with ESPB ( SMD = 1.89 , 95% CI (1.03, 2.74); p < 0.01 ). Conclusion. EPSB provides effective and safe postoperative analgesia after lumbar spine surgery.

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Tetrahydrobiopterin in antenatal brain hypoxia-ischemia-induced motor impairments and cerebral palsy

Cited by 34 publications

References 72 publications

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1

S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1

Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection—Protocol Optimization and Pitfalls

Efficacy of Postoperative Analgesia by Erector Spinal Plane Block after Lumbar Surgery: A Systematic Review and Meta-analysis of Randomized Controlled Trials

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