Tetrahydrofuran as solvent in dental adhesives: cytotoxicity and dentin bond stability

Fontes, Sílvia Terra; Fernandez, Matheus dos Santos; Ogliari, Fabrício Aulo; Carvalho, Rodrigo Varella de; Moraes, Rafael R.; Pinto, Márcia Bueno; Piva, Evandro

doi:10.1007/s00784-012-0693-5

Cited by 16 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The MTT assay has been widely used as a method to assess cell viability through the production of water soluble products with increased sensitivity and higher flexibility in the operation as well as cost and time efficiency [ 48 ]. To quantitatively estimate living cells, the amount of formazan crystals produced during metabolic processes in active NIH 3T3 cell lines is determined [ 49 ]. L. chinense leaf extracts reportedly exhibit higher cytotoxic effects in cancer cells [ 50 ].…”

Section: Resultsmentioning

confidence: 99%

Assessment of Mineral and Phenolic Profiles and Their Association with the Antioxidant, Cytotoxic Effect, and Antimicrobial Potential of Lycium chinense Miller

Thiruvengadam

Ghimire

Kim

et al. 2020

Plants

View full text Add to dashboard Cite

This study aimed at investigating the Lycium chinense Miller leaf extract mineral and phenolic compound profiles as well as antioxidant and antimicrobial potential. We determined the leaf extract mineral composition, identified its major mineral components, and quantified secondary metabolites. We also measured the leaf extract antioxidant potential and found that it varies in a concentration-dependent manner. We observed a significant and higher positive correlation between DPPH and ABTS assays compared with the total phenolic and flavonoid content. Furthermore, our assay results positively correlated with several observed acids, indicating their strong association with the L. chinense antioxidant potential. Our cytotoxic assay revealed weak toxicity at higher tested concentrations. Our MIC assay showed that the 80% methanol extract effectively inhibited the growth of Escherichia coli Castellani and Chalmers (ATCC35150). The 625-ppm leaf extract completely suppressed the growth of Staphylococcus aureus Rosenbach (ATCC13150), Bacillus cereus (ATCC 14579), and Helicobacter pylori (ATCC43504). These results allow us to understand the indigenous medicinal value of L. chinense. Our study suggests that the L. chinense leaf extract phenolic compounds possess a good antioxidant activity against free radicals and are effective antimicrobial agents. Finally, the presence and high level of diverse minerals suggest the potential of L. chinense for nutraceutical and functional food applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Assessment of Mineral and Phenolic Profiles and Their Association with the Antioxidant, Cytotoxic Effect, and Antimicrobial Potential of Lycium chinense Miller

Thiruvengadam

Ghimire

Kim

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…One disadvantage of the MTT assay is non-consistent estimation of number of viable cells in the resin content of tested materials [21] or the test itself [22] depending on the material tested, while several advantages of the test are obtaining rapid results, ease of application and visualization of cell density in small cell cultures [14,23,24]. In vitro studies that evaluated cytotoxicity of dental materials have commonly used fibroblast cells such as L929 and NIH/3T3 because of the reproducibility, availability and the resemblance of these cells to pulpal and gingival cells [1,6,[24][25][26][27]. Especially, NIH/3T3 which is one of the most commonly recommended cells for MTT assay, is a continuous cell line meaning that they can be reproduced fast and easy [26].…”

Section: Discussionmentioning

confidence: 99%

Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

et al. 2019

View full text Add to dashboard Cite

OBJECTIVES This study evaluated the cytotoxicity of resin-based luting cements on fibroblast cells using different polymerization protocols. MATERIALS AND METHODS Two conventional dual-polymerized (RelyX ARC, VariolinkN) and two self-adhesive resin cements (RelyX Unicem, Multilink Speed) specimens were polymerized using four different polymerization protocols: (a) photopolymerization with direct light application, (b) photo-polymerization over ceramic and (c) resin nanoceramic discs and (d) auto-polymerization. The specimens were then assigned to four groups to test cytotoxicity at 0, 1, 2 and 7 preincubation days (n = 5). MTT test was performed using NIH/3T3 fibroblast cells. Data were analysed using three-and one-way ANOVA. Multiple comparisons were made using Bonferroni post hoc test (p < 0.05). RESULTS The highest cytotoxic values were recorded at day 2 for conventional resin cements and at day 0 for self-adhesive resin cements. Self-adhesive resin cements showed the most cytotoxic effect at the second day, while conventional resin cements presented immediate cytotoxicity. Auto-polymerized resin specimens and especially Multilink Speed demonstrated the most cytotoxic effect regardless of the preincubation time. Cytotoxicity of cements tested reached the lowest level at day 7. Interposition of ceramic or nano-ceramic restorative material did not significantly affect the cytotoxicity of tested luting cements (p > 0.05). CONCLUSIONS Cytotoxicity of dual-polymerized resin cements was material-dependent and decreased gradually up to 7 days. Photo-polymerization plays an important role in reducing the cytotoxic effects. CLINICAL RELEVANCE When luting ceramic or resin nano-ceramic restorations of which thickness does not exceed 2 mm, the level of cytotoxicity with the tested materials is not significant. Luting of restorative materials that do not allow for light transmission such as metal-fused porcelain, clinicians should be cautious in the use of dual-polymerized conventional resin cements as only auto-polymerization of resin cements takes place under such materials.

show abstract

“…These results are important and reinforce that the adhesives tested were not cytotoxic in this assay. Although the components (HEMA, TEGDMA, phosphate monomer) might seem toxic after the initial monomer release ( 22 ), they did not interfere in cell growth, which may have led them to proliferate as they usually would in favorable conditions. Similar findings have been observed in previous studies using Easy Bond, with percentages of cell viability from 103.37% to 110.39%, depending on the methodology ( 23 , 24 ).…”

Section: Discussionmentioning

confidence: 99%

“…In accordance with recent studies, although no cytotoxic effects have been observed, the findings of SUB can be attributed to one of its components, the phosphate monomer. Amongst seven different solvents, it has been considered the most cytotoxic followed by HEMA, THF, acetone and ethanol ( 22 ).…”

Section: Discussionmentioning

confidence: 99%

Citotoxicity evaluation of three dental adhesives on vero cells in vitro

et al. 2016

View full text Add to dashboard Cite

BackgroundTo evaluate, in vitro, the potential cytotoxicity of three different dental adhesives systems (Adper Single Bond 2 -SB, Silorane System Adhesive Bond -SSAB and Single Bond Universal -SBU) on cultivated Vero cells after different contact times.Material and MethodsThe cells were cultured in a concentration of 2 x 105 cells/mL for 24h and grown to sub-confluent monolayers. VERO cells were exposed to 25µl of conditioned extracts obtained from 24h, 48h and 72h immersion of adhesive samples in culture medium (DMEM), immediately after polymerization. Fresh DMEM was used as negative control. Cell metabolism was evaluated by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2, 5diphenyl-tetrazolium bromide). The data were analyzed statistically by ANOVA, considering a significance of 5%.ResultsThe values of cell viability ranged from 94.2% at 72h (SBU) to 109.6% at 48h (SB). The mean percentage of viability after exposure to the extracts of SB, SSAB and SBU were 103.2%, 100.63% and 97.43%, respectively. There was no statistically significant difference (p= 0.342) between the experimental and negative control groups.ConclusionsAt all exposure times, all adhesives tested in this study presented no cytotoxicity to Vero cells in vitro. Key words:Biocompatibility, cytotoxicity, dental adhesives, Vero cells.

show abstract

Tetrahydrofuran as solvent in dental adhesives: cytotoxicity and dentin bond stability

Cited by 16 publications

References 25 publications

Assessment of Mineral and Phenolic Profiles and Their Association with the Antioxidant, Cytotoxic Effect, and Antimicrobial Potential of Lycium chinense Miller

Assessment of Mineral and Phenolic Profiles and Their Association with the Antioxidant, Cytotoxic Effect, and Antimicrobial Potential of Lycium chinense Miller

Effect of various polymerization protocols on the cytotoxicity of conventional and self-adhesive resin-based luting cements

Citotoxicity evaluation of three dental adhesives on vero cells in vitro

Contact Info

Product

Resources

About