Tetrahydroporphyrin-tetratosylate (THPTS)-based photodynamic inactivation of critical multidrug-resistant bacteria in vitro

Ziganshyna, Svitlana; Guttenberger, Anna; Lippmann, Norman; Schulz, Sebastian; Bercker, Sven; Kahnt, Axel; Rüffer, Tobias; Voigt, Alexander; Gerlach, Khrystyna; Werdehausen, Robert

doi:10.1016/j.ijantimicag.2020.105976

Cited by 9 publications

(11 citation statements)

References 32 publications

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“…Modified translucent hydrogels can serve as a carrier system, allowing the release of PSs under local illumination [ 44 , 45 ] . This, and the increasing availability of inexpensive lasers and light-emitting diodes (LED) for optical excitation [ 46 , 47 ], may enable applications of PDT both in cancer therapy and against infections with multidrug-resistant bacteria.…”

Section: Introductionmentioning

confidence: 99%

The Meta-Substituted Isomer of TMPyP Enables More Effective Photodynamic Bacterial Inactivation than Para-TMPyP In Vitro

et al. 2022

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Porphyrinoid-based photodynamic inactivation (PDI) provides a promising approach to treating multidrug-resistant infections. However, available agents for PDI still have optimization potential with regard to effectiveness, toxicology, chemical stability, and solubility. The currently available photosensitizer TMPyP is provided with a para substitution pattern (para-TMPyP) of the pyridinium groups and has been demonstrated to be effective for PDI of multidrug-resistant bacteria. To further improve its properties, we synthetized a structural variant of TMPyP with an isomeric substitution pattern in a meta configuration (meta-TMPyP), confirmed the correct structure by crystallographic analysis and performed a characterization with NMR-, UV/Vis-, and IR spectroscopy, photostability, and singlet oxygen generation assay. Meta-TMPyP had a hypochromic shift in absorbance (4 nm) with a 55% higher extinction coefficient and slightly improved photostability (+6.9%) compared to para-TMPyP. Despite these superior molecular properties, singlet oxygen generation was increased by only 5.4%. In contrast, PDI, based on meta-TMPyP, reduced the density of extended spectrum β-lactamase-producing and fluoroquinolone-resistant Escherichia coli by several orders of magnitude, whereby a sterilizing effect was observed after 48 min of illumination, while para-TMPyP was less effective (p < 0.01). These findings demonstrate that structural modification with meta substitution increases antibacterial properties of TMPyP in PDI.

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Section: Introductionmentioning

confidence: 99%

The Meta-Substituted Isomer of TMPyP Enables More Effective Photodynamic Bacterial Inactivation than Para-TMPyP In Vitro

et al. 2022

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“…Both photosensitizers have been reported to have antibacterial properties previously. [19][20][21] Additionally, both photosensitizers are cationic. While anionic photosensitizers are ineffective against several bacteria, cationic photosensitizers have shown high efficiency in the past.…”

Section: Introductionmentioning

confidence: 99%

Photosensitizer-loaded hydrogels for photodynamic inactivation of multirestistant bacteria in wounds

et al. 2021

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“…The feasibility of this approach was shown to depend, on the one hand, on the bacterial strain used, and on the other hand, on the optical properties of the PS. We found that the investigated luminescence-based method is feasible in many of the bacterial strains tested, and the aPDI effects determined by this approach were in good agreement with those obtained by manual counting of bacterial colonies after agar plate culturing [ 32 ]. In some bacterial strains, such as MDR Pseudomonas aeruginosa , however, the intrinsic properties of the bacteria precluded the use of luminescence-based cell density determination.…”

Section: Discussionmentioning

confidence: 68%

“…For TMPyP-treated samples, illumination was started for 0, 60, 90 and 120 min, as previously described [ 30 , 31 ]. For THPTS-based aPDI treatment, bacteria were illuminated for 0, 4, 12, 36 and 108 min in accordance with Ziganshyna et al [ 32 ]. Additional samples were stored under identical conditions but protected from light (dark control).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Luminometric Cell Counting System in Context of Antimicrobial Photodynamic Inactivation

et al. 2022

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Antimicrobial resistance belongs to the most demanding medical challenges, and antimicrobial photodynamic inactivation (aPDI) is considered a promising alternative to classical antibiotics. However, the pharmacologic characterization of novel compounds suitable for aPDI is a tedious and time-consuming task that usually requires preparation of bacterial cultures and counting of bacterial colonies. In this study, we established and utilized a luminescence-based microbial cell viability assay to analyze the aPDI effects of two porphyrin-based photosensitizers (TMPyP and THPTS) on several bacterial strains with antimicrobial resistance. We demonstrate that after adaptation of the protocol and initial calibration to every specific bacterial strain and photosensitizer, the luminometric method can be used to reliably quantify aPDI effects in most of the analyzed bacterial strains. The interference of photosensitizers with the luminometric readout and the bioluminescence of some bacterial strains were identified as possible confounders. Using this method, we could confirm the susceptibility of several bacterial strains to photodynamic treatment, including extensively drug-resistant pathogens (XDR). In contrast to the conventional culture-based determination of bacterial density, the luminometric assay allowed for a much more time-effective analysis of various treatment conditions. We recommend this luminometric method for high-throughput tasks requiring measurements of bacterial viability in the context of photodynamic treatment approaches.

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Tetrahydroporphyrin-tetratosylate (THPTS)-based photodynamic inactivation of critical multidrug-resistant bacteria in vitro

Cited by 9 publications

References 32 publications

The Meta-Substituted Isomer of TMPyP Enables More Effective Photodynamic Bacterial Inactivation than Para-TMPyP In Vitro

The Meta-Substituted Isomer of TMPyP Enables More Effective Photodynamic Bacterial Inactivation than Para-TMPyP In Vitro

Photosensitizer-loaded hydrogels for photodynamic inactivation of multirestistant bacteria in wounds

Evaluation of a Luminometric Cell Counting System in Context of Antimicrobial Photodynamic Inactivation

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