Tetrahydroxystilbene glucoside alleviates angiotensin II induced HUVEC senescence via SIRT1

Abstract

Tetrahydroxystilbene glucoside (TSG), an active ingredient of <i>Polygonum multiflorum</i>, has been known for certain anti-aging effects. In this study, the possible protective mechanism of TSG on human umbilical vein endothelial cells (HUVECs) senescence induced by angiotensin Ⅱ (Ang Ⅱ) was investigated. The results revealed that TSG pretreatment could reduce the percentage of senescence-associated-β-galactosidase (SA-β-gal) positive cells, and decrease the expression levels of the cellular senescence biomarkers, p53 and PAI-1 proteins. At the same time, the expression of SIRT1 in senescent cell showed an upward trend due to TSG treatment. When inhibiting the expression of SIRT1 by EX527, our results showed that TSG reversed the effect of EX527, by promoting the expression level of SIRT1, reducing the expression of SA-β-gal positive cell and the expression level of p53 and PAI-1 proteins. The present study demonstrated that TSG could protect against HUVECs senescence induced by Ang Ⅱ, potentially through modulation of SIRT1 activity.

Pharmacokinetics, Tissue Distribution, and Excretion Characteristics of a Radix Polygoni Multiflori Extract in Rats

“…Moldenke (Polygonaceae), is a popular traditional Chinese medicine that has been used for hair darkening, prolonging life, and mitigating several dysfunctions ( Tang et al, 2017 ). Modern pharmacological research and clinical practices have shown that RPM has several bioactive properties, including antioxidant ( Liu X. et al, 2021 ; Wang et al, 2008 ), antiaging ( Cheung et al, 2014 ; Fan et al, 2021 ), and antitumor activities ( Ma et al, 2012 ; Hou et al, 2013 ; Way et al, 2014 ; Lin et al, 2017 ; Xing et al, 2019 ), and it helps in regulating immunity ( Chen et al, 2012 ), lowering blood lipids ( Yao et al, 2013 ; Chang et al, 2016 ; Xie et al, 2012 ), and promoting neuroprotection ( Sun et al, 2011 ; Yang X.-P. et al, 2014 ; Ruan et al, 2021 ). However, an increasing number of recently published studies have demonstrated the adverse effects of RPM.…”

Cellular Senescence in Cardiovascular Diseases: A Systematic Review

“…The decreased SIRT1 expression and activity were closely associated with the progression of cellular senescence and cardiac dysfunction [ 115 ]. Conversely, SIRT1 activation confers protective effects on cellular senescence involving cardiovascular system [ 116 - 118 ]. Although the mechanisms remain unclear, SIRT1 inhibition-mediated p53 acetylation was implicated in aging-related cardiovascular disorders.…”

A Review of Pharmacology, Toxicity and Pharmacokinetics of 2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-Glucoside

“…Further studies have shown that the mechanism of its anti-cutaneous aging effect is mainly through inhibiting the insulin/IGF-1 signaling pathway ( Zhou et al, 2014 ). In the angiotensin II-induced senescence model of HUVECs, TSG (20, 40 μg/ml) treatment decreased the percent of senescence-associated-β-galactosidase positive cells and the expression levels of cellular senescence biomarkers, p53 and PAI-1 protein ( Fan et al, 2021 ). Its anti-aging mechanism is mainly attributed to the regulation of SIRT1 activity ( Fan et al, 2021 ).…”

“…In the angiotensin II-induced senescence model of HUVECs, TSG (20, 40 μg/ml) treatment decreased the percent of senescence-associated-β-galactosidase positive cells and the expression levels of cellular senescence biomarkers, p53 and PAI-1 protein ( Fan et al, 2021 ). Its anti-aging mechanism is mainly attributed to the regulation of SIRT1 activity ( Fan et al, 2021 ). Similarly, in the mouse model established by high calorie diet, TSG delayed the aging of aged mice consuming excess calories ( Ning et al, 2018 ).…”

“…Modern pharmacological research has shown that TSG has significant pharmacological effects, including anti-inflammation ( Chin et al, 2016 ; Li et al, 2018 ), antioxidation ( Wen et al, 2020 ; Wu et al, 2020 ), neuroprotection ( Gao et al, 2020 ; Tong et al, 2020 ), cardiovascular protection ( Hu et al, 2019 ; Jia et al, 2019 ), hepatoprotection ( Wang et al, 2020a ; Zhang et al, 2020 ), anti-osteoporosis ( Zheng et al, 2017 ; Zhang et al, 2018a ), memory enhancement ( Hou et al, 2011 ; Chen et al, 2016 ), and anti-aging ( Zhang et al, 2013c ; Fan et al, 2021 ), which largely contribute to the prevention and treatment of various diseases, such as inflammatory diseases, neurodegenerative diseases, cardiovascular diseases, liver injury, osteoporosis, and diabetic nephropathy. More importantly, nuclear factor kappa-B (NF-κB) ( Lin et al, 2015b ), Adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) ( Ning et al, 2018 ), Phosphatidylinositide 3-kinase (PI3K)-protein kinase B (AKT) ( Zhang et al, 2013b ), reactive oxygen species (ROS)-nitric oxide (NO) ( Tao et al, 2011 ), B-cell lymphoma-2 (Bcl-2)/Bcl-2-associated X protein (Bax)/Caspase-3 ( Zhou et al, 2020 ), and transforming growth factor-β (TGF-β)/Smad signaling pathways ( Yao et al, 2015 ), have been shown to mediate the therapeutic effects of TSG.…”