Tetramethylpyrazine alleviated cytokine synthesis and dopamine deficit and improved motor dysfunction in the mice model of Parkinson’s disease

Hong, Zhao; Xu, Meiling; Zhou, Qi Tony; Guo, Zhang; Peng, Ying; Qu, Zhengyi; Li, Yongnan

doi:10.1007/s10072-014-1871-9

Cited by 20 publications

(8 citation statements)

References 17 publications

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“…The results revealed that stimulation with 1 µg/ml LPS for 24 h significantly inhibited cell viability. Previous studies have demonstrated that TMP exerts anti-inflammatory effects on hepatic stellate cells, a mouse model of Parkinson's disease and myocardial cells (39)(40)(41). The present study demonstrated that inflammation levels induced by LPS were significantly reduced by TMP in a concentration-dependent manner.…”

Section: Discussionsupporting

confidence: 68%

Tetramethylpyrazine reduces inflammation levels and the apoptosis of LPS‑stimulated human periodontal ligament cells via the downregulation of miR‑302b

Duan

et al. 2020

Int J Mol Med

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Periodontitis is the main cause of tooth or tissue loss. Human periodontal ligament stem cells (hPdLScs), which have high proliferative, self-renewal and multi-differentiation abilities, are vital for the restoration of periodontitis-induced injuries. The anti-inflammatory and anti-apoptotic agent, tetramethylpyrazine (TMP), is a promising agent used for the protection of PdLScs from apoptosis and inflammation induced by periodontitis. The aim of the present study was to investigate the effects of TMP on lipopolysaccharide (LPS)-stimulated hPdLScs. LPS-stimulated hPdLScs were established as the cell model. ccK-8 assay was performed to evaluate cell viability, western blot analysis was performed to measure protein expression and flow cytometry was performed to detect cell apoptosis levels. detection kits were used to evaluate the levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6. Reverse transcription-quantitative PcR analysis was performed to detect gene expression. TMP alleviated the effects of LPS on cell viability, inflammation levels and cell apoptosis. TMP downregulated microRNA (miR)-302b levels in LPS-stimulated cells. Transfection with miR-302b mimic reversed the anti-inflammatory and anti-apoptotic effects of TMP on LPS-stimulated cells. TMP reduced inflammation and the apoptosis of LPS-stimulated human periodontal ligament cells via the downregulation of miR-302b. The anti-inflammatory and anti-apoptotic effects exerted by TMP render it a promising agent for the protection of PdLScs from injuries induced by periodontitis. The findings of the present study may aid in the development of a novel strategy for the treatment of periodontitis and may pave the way for further research.

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Section: Discussionsupporting

confidence: 68%

Tetramethylpyrazine reduces inflammation levels and the apoptosis of LPS‑stimulated human periodontal ligament cells via the downregulation of miR‑302b

Duan

et al. 2020

Int J Mol Med

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“…Previous studies have evidenced that TMP has the pharmacological activity of anti-platelet aggregation and the physiological functions of softening the blood vessels, increasing coronary blood flow, improving microcirculation and scavenging free radicals [2][3][4][5]. It has been used in clinical applications as a medicinal agent with high security for more than 30 years, especially in the treatment of patients with cerebral ischemic diseases, because of its neuroprotective effects and global cerebral ischemia [6,7].…”

Section: Article; Pharmaceutical Biotechnologymentioning

confidence: 99%

Screening and identification ofMonacusstrain with high TMP production and statistical optimization of its culture medium composition and liquid state fermentation conditions using response surface methodology (RSM)

Jia

Guo

Zhou

et al. 2017

Biotechnology & Biotechnological Equipment

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Cong Lv (2017) Screening and identification of Monacus strain with high TMP production and statistical optimization of its culture medium composition and liquid state fermentation conditions using response surface methodology (RSM), Biotechnology & Biotechnological Equipment, 31:4, 828-838, DOI: 10.1080/13102818.2017 C, fermentation time of 9 d, shaker speed of 180 r/min, inoculum size of 7.21% and loading volume of 100 mL/250 mL triangular flask. Under the optimum composition of culture medium and liquid state fermentation conditions, the TMP production of Monacus strain M-3 can reach 13.49 mg/mL. This study is expected to provide a new approach of TMP production through biosynthesis by Monascus strain, and promote the further development of functional foods or medicines from Monascus fermentation products.

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“…However, it must be mentioned that we could not exclude downstream signaling pathway suppression, such as CaMKII. Previous studies have demonstrated that TMP could inhibit the activation of the calcium/calmodulin/calmodulin-dependent protein kinase (Ca2+/CaM/CaMKII) pathway [ 27 ]. TMP may suppress CaMKII to attenuate neuropathic pain.…”

Section: Discussionmentioning

confidence: 99%

Selective suppression of the JNK-MMP2/9 signal pathway by tetramethylpyrazine attenuates neuropathic pain in rats

Jiang

Pan

Wang

et al. 2017

J Neuroinflammation

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Background: Activated astrocytes release matrix metalloproteinase-2/9 (MMP-2/9) to induce central sensitization and maintain neuropathic pain. However, the mechanisms involved in the activation of MMP-2/9 on astrocytes during pain remain poorly understood. Meanwhile, there is a lack of effective treatment to inhibit the activation of MMP-2/9 on astrocytes. In this study, we aim to investigate the effect of tetramethylpyrazine (TMP), a natural compound with analgesic effects but unknown mechanisms, on MMP-2/9 in neuropathic pain. Methods: The nociception was assessed by measuring the incidence of foot withdrawal in response to mechanical indentation in rats (n = 6). Cell signaling was assayed using western blotting (n = 6) and immunohistochemistry (n = 5). The astrocyte cell line C8-D1A was cultured to investigate the in vitro effects. Results: TMP significantly attenuated the maintenance of chronic constrictive injury (CCI)-induced neuropathic pain, inhibited the activation of astrocytes, and decreased the expression of MMP-2/9. Furthermore, our results indicated that TMP could selectively suppress JNK activity but had no notable effects on ERK and p38. Our study also revealed that the effect of TMP may be dependent on the inhibition of TAK1.

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Tetramethylpyrazine alleviated cytokine synthesis and dopamine deficit and improved motor dysfunction in the mice model of Parkinson’s disease

Cited by 20 publications

References 17 publications

Tetramethylpyrazine reduces inflammation levels and the apoptosis of LPS‑stimulated human periodontal ligament cells via the downregulation of miR‑302b

Tetramethylpyrazine reduces inflammation levels and the apoptosis of LPS‑stimulated human periodontal ligament cells via the downregulation of miR‑302b

Screening and identification ofMonacusstrain with high TMP production and statistical optimization of its culture medium composition and liquid state fermentation conditions using response surface methodology (RSM)

Selective suppression of the JNK-MMP2/9 signal pathway by tetramethylpyrazine attenuates neuropathic pain in rats

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