Tetramethylpyrazine protects spinal cord and reduces inflammation in a rat model of spinal cord ischemia-reperfusion injury

Fan, Lihong; Wang, Kunzheng; Shi, Zhibin; Die, Jun; Wang, Chunsheng; Dang, Xiaoqian

doi:10.1016/j.jvs.2010.12.030

Cited by 84 publications

(57 citation statements)

References 35 publications

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“…5,6 SUMOylation is involved in many of the processes activated in the post-ischemic spinal cord. These include DNA damage repair, 16,17 ubiquitin conjugation and proteasomal degradation of proteins, 18,19 NF-kB activation, 20,21 hypoxia-inducible factor activation 22,23 and oxidative stress. 24,25 Therefore, the post-ischemic activation of SUMO2/3 conjugation is highly likely to have a major impact on the fate of post-ischemic neurons.…”

Section: Discussionmentioning

confidence: 99%

Transient ischemia induces massive nuclear accumulation of SUMO2/3-conjugated proteins in spinal cord neurons

Wang

Sheng

et al. 2012

Spinal Cord

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Objectives: The objective of this study is to determine whether transient spinal cord ischemia activates small ubiquitin-like modifier (SUMO1-3) conjugation, a post-translational protein modification that protects neurons from ischemia-like conditions. Methods: Mice were subjected to 8-12 min of spinal cord ischemia and 3-24 h of recovery using a newly developed experimental model. To characterize the model, activation of stress response pathways induced after spinal cord ischemia, previously observed in other experimental models, was verified by western blot analysis. Levels and subcellular localization of SUMO-conjugated proteins in spinal cords were evaluated by western blot analysis and immunohistochemistry, respectively. Results: Following transient spinal cord ischemia, stress responses were activated as indicated by increased phosphorylation of eukaryotic initiation factor 2 (eIF2a), extracellular signal-regulated kinases (ERK1/2) and Akt. SUMO1 conjugation was not altered, but a selective rise in levels of SUMO2/3-conjugated proteins occurred, peaking at 6 h reperfusion. The marked activation of SUMO2/ 3 conjugation was a neuronal response to ischemia, as indicated by co-localization with the neuronal marker NeuN, and was associated with nuclear accumulation of SUMO2/3-conjugated proteins. Conclusion: Our study suggests that spinal cord neurons respond to ischemic stress by activation of SUMO2/3 conjugation. Many of the identified SUMO target proteins are transcription factors and other nuclear proteins involved in gene expression and genome stability. It is therefore concluded that the post-ischemic activation of SUMO2/3 conjugation may define the fate of neurons exposed to a transient interruption of blood supply, and that this pathway could be a therapeutic target to increase the resistance of spinal cord neurons to transient ischemia.

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Section: Discussionmentioning

confidence: 99%

Transient ischemia induces massive nuclear accumulation of SUMO2/3-conjugated proteins in spinal cord neurons

Wang

Sheng

et al. 2012

Spinal Cord

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“…TMP has been identified with several molecular targeting properties, and demonstrates a broad therapeutic capacity such as scavenging oxygen free radicals (Li et al, 2009;Fan et al, 2011). It has antiapoptotic effect associated with down-regulating the expressions of Bcl-2 and Bcl-2 Associated X protein (bax) along with suppressing of caspase-3 (Chang et al, 2007).…”

Section: Research Articlementioning

confidence: 99%

“…On day 7 post hatch, after induction of TD, thiram group was equally divided into two groups designated as TD group (n=60) and TMP treatment group (n=60). TMP group was treated with Tetramethylpyrazine @ 30 mg/kg/day (Fan et al, 2011;Shin et al, 2013;Zhang et al, 2015) by intraperitoneal route from day 7 to day 18.…”

Section: Experiments Designmentioning

confidence: 99%

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Mehmood¹

2018

PVJ

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“…Previous investigations have also demonstrated that increased oxidative stress, neuroinflammation, and apoptotic cell death are associated with SCII [6, 7]. In particular, numerous studies have established that SCII is intimately linked to inflammatory responses and the upregulation of proinflammatory cytokines and chemokines [8, 9]; therefore, natural products containing bioactive molecules with anti-inflammatory properties possess potential as an alternative approach to improve SCII-induced neurological deficits and tissue injuries [10-12]. …”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Effect of Lycopene on Experimental Spinal Cord Ischemia Injury via Cyclooxygenase-2 Suppression

et al. 2019

Neuroimmunomodulation

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Objective: Spinal cord ischemia/reperfusion injury (SCII) is a devastating complication following thoracoabdominal aortic surgeries, often leading to severe neurological deficits. We sought to examine the effects of lycopene, a naturally existing carotenoid with anti-inflammatory properties, in the treatment against SCII. Methods: Rats were assigned into four treatment groups: Sham (sham operation), SCII (SCII-induction), LY25, and LY50 (lycopene treatment at 25 or 50 mg/kg following SCII induction, respectively). Results: Lycopene treatment improved the recovery of neurological functions following SCII and suppressed the neuronal cell death and neuroinflammation at 14 days after SCII. Furthermore, Western blot assay revealed that lycopene treatment attenuated the SCII-induced increase in the protein levels of cyclooxygenase-2 (COX-2), nuclear factor-κB, and activate protein-1, as well as the reduction of heme oxygenase-1. Conclusion: Lycopene exerted neuroprotective functions in SCII and inhibited SCII-elicited neuroinflammation via COX-2 suppression.

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Tetramethylpyrazine protects spinal cord and reduces inflammation in a rat model of spinal cord ischemia-reperfusion injury

Abstract: Treatment with TMP exerted a neuroprotective effect against spinal cord ischemia-reperfusion injury. The anti-inflammatory effect was believed to be one of the contributing mechanisms.

Cited by 84 publications

References 35 publications

Transient ischemia induces massive nuclear accumulation of SUMO2/3-conjugated proteins in spinal cord neurons

Transient ischemia induces massive nuclear accumulation of SUMO2/3-conjugated proteins in spinal cord neurons

Tetramethylpyrazine Mitigates Toxicity and Liver Oxidative Stress in Tibial Dyschondroplasia Chickens

Anti-Inflammatory Effect of Lycopene on Experimental Spinal Cord Ischemia Injury via Cyclooxygenase-2 Suppression

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