Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents

He, Qiong; Zhu, Jinxia; Xing, Ying; Tsang, Lai-Ling; Yang, Ning; Rowlands, Dewi K.; Chung, Yiu-Wa; Chan, Hsiao-Chang

doi:10.3748/wjg.v11.i27.4173

Cited by 5 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In myometrial cultures, both L. wallichii and TMP were unable to increase adenylate cyclase activity above basal levels, suggesting that neither can directly stimulate cAMP production in this tissue preparation. This is rather unexpected since we have previously shown that TMP can stimulate colonic anion secretion via cAMP pathway (He et al, 2005).…”

Section: Discussionmentioning

confidence: 91%

Traditional Chinese medicine Bak Foong Pills alters uterine quiescence – Possible role in alleviation of dysmenorrhoeal symptoms

Rowlands

Cui

Wong

et al. 2009

Cell Biology International

View full text Add to dashboard Cite

Since contractility of the uterus appears to be the major source of pain during dysmenorrhoea, alleviation of the contractions is believed to be a possible treatment strategy. Bak Foong Pills, a traditional Chinese formulation for use in gynaecological disorders, has long been thought as effective in the treatment of dysmenorrhoeal symptoms. The present study thus aims to investigate whether ethanol extract of Bak Foong Pills (BFP-Ex) or its constituent herbs may have direct effects on alleviating dysmenorrhoeal symptoms by altering uterine tone. This was investigated using isolated uterine preparations and intracellular messenger analysis of adenylate cyclase, via [(3)H]-adenine assay, and calcium, with fluorometry imaging, in myometrial cultures. BFP-Ex can stimulate uterine relaxation following oxytocin-induced contractions ex-vivo. Attempted inhibition of BFP-Ex's relaxatory response with a nitric oxide inhibitor and adenylate cyclase inhibitor, however, had no significant effect, suggesting that most of BFP-Ex's relaxatory response was not due to increases in NO or cAMP. Further studies on tetramethylpyrazine (TMP), a major active ingredient of BFP-Ex, indicated that TMP could modulate intracellular calcium levels in favour of uteri relaxation. The ability of Bak Foong Pills to alleviate menstrual pain may be due to direct regulation of uterine tone.

show abstract

Section: Discussionmentioning

confidence: 91%

Traditional Chinese medicine Bak Foong Pills alters uterine quiescence – Possible role in alleviation of dysmenorrhoeal symptoms

Rowlands

Cui

Wong

et al. 2009

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Measurements were performed in the presence of a transepithelial Cl − gradient, in which the basolateral side solution contained (in mmol/L): 130 NaCl, 2.7 KCl, 1.5 KH 2 PO 4 , 1 CaCl 2 , 0.5 MgCl 2 , 10 Na–HEPES, pH 7.3, and 10 glucose; The apical side solution contained the same components except that 65 mmol/L NaCl was replaced by sodium gluconate and the concentration of CaCl 2 was increased to 2 mmol/L. The basolateral membrane was permeabilized with 250 μg/ml amphotericin B (He et al, 2005 ).…”

Section: Methodsmentioning

confidence: 99%

Stimulation of Airway and Intestinal Mucosal Secretion by Natural Coumarin CFTR Activators

Yang¹,

Xu²,

Sui³

et al. 2011

Front. Pharmacol.

View full text Add to dashboard Cite

Mutations of cystic fibrosis (CF) transmembrane conductance regulator (CFTR) cause lethal hereditary disease CF that involves extensive destruction and dysfunction of serous epithelium. Possible pharmacological therapy includes correction of defective intracellular processing and abnormal channel gating. In a previous study, we identified five natural coumarin potentiators of ΔF508-CFTR including osthole, imperatorin, isopsoralen, praeruptorin A, and scoparone. The present study was designed to determine the activity of these coumarine compounds on CFTR activity in animal tissues as a primary evaluation of their therapeutic potential. In the present study, we analyzed the affinity of these coumarin potentiators in activating wild-type CFTR and found that they are all potent activators. Osthole showed the highest affinity with Kd values <50 nmol/L as determined by Ussing chamber short-circuit current assay. Stimulation of rat colonic mucosal secretion by osthole was tested by the Ussing chamber short-circuit current assay. Osthole reached maximal activation of colonic Cl− secretion at 5 μmol/L. Stimulation of mouse tracheal mucosal secretion was analyzed by optical measurement of single gland secretion. Fluid secretion rate of tracheal single submucosal gland stimulated by osthole at 10 μmol/L was three-fold more rapid than that in negative control. In both cases the stimulated secretions were fully abolished by CFTRinh-172. In conclusion, the effective stimulation of Cl– and fluid secretion in colonic and tracheal mucosa by osthole suggested the therapeutic potential of natural coumarin compounds for the treatment of CF and other CFTR-related diseases.

show abstract

“…The values are means ± SE, *P < 0.05, **P < 0.01. bacteria. In a previous study, it has been proved that TMP, which has been widely used clinically, could promote the secretion of HCO 3 - [8]. It is well known that the epithelial HCO 3 -secretion needs two steps: transportation through basolateral membrane and exit through apical membrane.…”

Section: Dicussionmentioning

confidence: 99%

“…The basolateral presence of bumetanide (0.1 mmol/l), the inhibitor of Na Recently it has been reported that TMP could increase the anion secretion of colonic mucosa [8].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Underlying Cellular Mechanism in the Effect of Tetramethylpyrazine on the Anion Secretion of Colonic Mucosa

Zhao

Duan

Wang

et al. 2005

JJP

View full text Add to dashboard Cite

Abstract:The present study investigated the underlying cellular mechanism in the effect of ligustrazine (tetramethylpyrazine, TMP) on the anion secretion of colonic mucosa in rats using a short-circuit current (I sc ) technique in conjunction with "tool drugs." (i) After a pretreatment of the tissues by bathing the bilateral surface with Cl --free Krebs-Henseleit (K-H) solution for over an hour, a basolateral application of 1 mmol/l TMP produced an increase in I sc , and the total charges transported for 30 min were about 8.7 ± 1.4 mC/cm 2 ; an apical pretreatment of DPC and a basolateral addition of acetazolamide decreased the TMP-induced I sc by about 60% (P < 0.01) and 45% (P < 0.05), respectively; a basolateral application of 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), the inhibitor of Na

show abstract

Tetramethylpyrazine stimulates cystic fibrosis transmembrane conductance regulator-mediated anion secretion in distal colon of rodents

Abstract: TMP may stimulate cAMP-dependent and CFTR-mediated Cl(-) and HCO(3)(-) secretion. This may have implications in the future development of alternative treatment for constipation.

Cited by 5 publications

References 34 publications

Traditional Chinese medicine Bak Foong Pills alters uterine quiescence – Possible role in alleviation of dysmenorrhoeal symptoms

Traditional Chinese medicine Bak Foong Pills alters uterine quiescence – Possible role in alleviation of dysmenorrhoeal symptoms

Stimulation of Airway and Intestinal Mucosal Secretion by Natural Coumarin CFTR Activators

The Underlying Cellular Mechanism in the Effect of Tetramethylpyrazine on the Anion Secretion of Colonic Mucosa

Contact Info

Product

Resources

About