Tetrandrine ameliorates collagen-induced arthritis in mice by restoring the balance between Th17 and Treg cells via the aryl hydrocarbon receptor

Yuan, Xusheng; Tong, Bei; Dou, Youjun; Wu, Xin; Wei, Zhifeng; Dai, Yue

doi:10.1016/j.bcp.2015.11.025

Cited by 64 publications

(53 citation statements)

References 43 publications

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“…Tetrandrine, a bisbenzylisoquinoline alkaloid, is isolated from the roots of Stephania tetrandra (4). Numerous studies have been reported that tetrandrine can be broadly used for patients with arthritis, hypertension, sepsis, inflammation and silicosis (5)(6)(7)(8)(9). Additionally, tetrandrine has been found to inhibit proliferation and induce apoptosis in various types of cancer cells, such as gastric and colon cancer, hepatocellular and renal cell carcinoma (10)(11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

Autophagy induction enhances tetrandrine-induced apoptosis via the AMPK/mTOR pathway in human bladder cancer cells

Kou

Liu

et al. 2017

Oncology Reports

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Tetrandrine, a bisbenzylisoquinoline alkaloid isolated from the roots of Stephania tetrandra is a traditional Chinese medicine and exerts anticancer capacity in various types of cancers. Previous studies have shown that tetrandrine induces apoptosis in bladder cancer cells via activation of the caspase cascade. However, the underlying mechanism has not yet been reported. Autophagy is a cellular process involved in the degradation of broken proteins and aging organelles to maintain homeostasis. Recent studies indicate that autophagy is implicated in cancer therapy. Thus, we focused on the correlation between autophagy and apoptosis upon tetrandrine treatment in human bladder cancer cells. Firstly, our results observed a marked increase in autophagic double-membrane vacuoles and fluorescent puncta of red fluorescence protein-green fluorescence protein-LC3 (GRP-RFP-LC3) upon tetrandrine treatment, as evidenced by transmission electron microscopy and confocal fluorescence microscopy. Secondly, the expression of LC3-II was increased in tetrandrine-treated T24 and 5637 cells in a time- and concentration-dependent manner. Subsequently, downregulation of p62 and LC3 turnover assay further confirmed that tetrandrine induced autophagic flux in bladder cancer T24 and 5637 cells. Thirdly, the protein levels of phosphorylated-AMP-activated protein kinase (AMPK) and phosphorylated-acetyl-coenzyme A carboxylase (ACC) were upregulated in the tetrandrine-treated cells, while the mammalian target of rapamycin (mTOR)-related proteins were downregulated. Moreover, AICAR, a common AMPK activator, further increased the expression the LC3-II, while AMPK inhibitor compound C partially reversed the LC3-II protein levels in bladder cancer T24 cells. Finally, AICAR significantly reinforced the growth inhibition and apoptosis induction of tetrandrine in T24 and 5637 cells, while compound C had an opposite effect, suggesting that AMPK-mediated autophagy enhanced the cytotoxic and pro-apoptosis effect of tetrandrine in human bladder cancer cells. Taken together, the present study showed that tetrandrine induced autophagy in human bladder cancer cells by regulating the AMPK/mTOR signaling pathway, which contributed to the apoptosis induction by tetrandrine, indicating that tetrandrine may be a potential anticancer candidate for the treatment of bladder cancer, and autophagy may be a possible mechanism for cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Autophagy induction enhances tetrandrine-induced apoptosis via the AMPK/mTOR pathway in human bladder cancer cells

Kou

Liu

et al. 2017

Oncology Reports

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“…Only two compounds, 9 and 52, have been evaluated for their antibacterial effects. Tetrandrine (9) (200.9, 401.9 or 803.8 lM) exhibited a synergistic effect against methicillin-resistant Staphylococcus aureus (MRSA) when combined with the multi-drug resistant (MDR)-efflux pump substrate ethidium bromide, causing a two to fourfold reduction in the minimum inhibitory concentration (MIC) of ethidium bromide against MRSA using a combined test (Lee et al 2011 Lai (2002), Sekiya et al (2004), Seow et al (1988), Lai et al (1999), Yun et al (2018), Yuan et al (2016), Lv et al (2015), Shan et al (2018), Hu et al (2012), Zhang et al (2018a, b, c, d), Zhang (2014), Sun et al (2016) Antifibrotic effects Antifibrotic effects in pulmonary disease . Though the compound exhibited potent inhibitory activity against S. aureus, the required dose of the two compounds was excessive in these studies, the experimental data not being relevant to clinical applications.…”

Section: Antibacterial Effectsmentioning

confidence: 99%

“…The compounds further restored the ratio of T helper 17 (Th17)/ regulatory T (Treg) cells. This has been demonstrated by measurement of serum concentrations of relevant cytokines (IL-17 and IL-10) and the proportion of each cell type (Yuan et al 2016). They have also reduced expression of Rac family small GTPase 1 (Rac1), cell division cycle 42 (Cdc42) and Ras homolog family member A (RhoA), and inhibited Akt and JNK activation, resulting in the subsequent down-regulation of MMP-2/9, F-actin and FAK activation and/or expression (Lv et al 2015).…”

Section: Immunomodulatory Effectsmentioning

confidence: 99%

A critical review: traditional uses, phytochemistry, pharmacology and toxicology of Stephania tetrandra S. Moore (Fen Fang Ji)

2020

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Stephania tetrandra S. Moore (S. tetrandra) is distributed widely in tropical and subtropical regions of Asia and Africa. The root of this plant is known in Chinese as ''Fen Fang Ji''. It is commonly used in traditional Chinese medicine to treat arthralgia caused by rheumatism, wet beriberi, dysuria, eczema and inflamed sores. Although promising reports have been published on the various chemical constituents and activities of S. tetrandra, no review comprehensively summarizes its traditional uses, phytochemistry, pharmacology and toxicology. Therefore, the review aims to provide a critical and comprehensive evaluation of the traditional use, phytochemistry, pharmacological properties, pharmacokinetics and toxicology of S. tetrandra in China, and meaningful guidelines for future investigations.

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“…Tetrandrine in the treatment of experimental auricular burns plays a direct excitatory adrenal function. Tetrandrine not only inhibits cyclooxygenase (COX)-2 and nitric oxide synthase (iNOS) by interfering with NF-κB/COX-2 signaling pathway, but also inhibits histamine, platelet-derived growth factor (PDGF), IL-1β, and TNF-α in inflammatory reactions and promotes the expression of the anti-inflammatory cytokine IL-10 [19].…”

Section: Tetrandrinementioning

confidence: 99%

Research Progress on Anti‐Inflammatory Effects and Mechanisms of Alkaloids from Chinese Medical Herbs

Liu

Chen

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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As the spectrum of diseases keeps changing and life pace keeps going faster, the probability and frequency of diseases caused by human inflammatory reactions also keep increasing. How to develop effective anti-inflammatory drugs has become the hotspot of researches. It has been found that alkaloids from Chinese medical herbs have anti-inflammatory, analgesic, antitumor, anticonvulsant, diuretic, and antiarrhythmic effects, among which the anti-inflammatory effect is very prominent and commonly used in the treatment of rheumatoid arthritis, ankylosing spondylitis, and other rheumatic immune diseases, but its mechanism of action has not been well explained. Based on this, this paper will classify alkaloids according to structural types and review the plant sources, applicable diseases, and anti-inflammatory mechanisms of 16 kinds of alkaloids commonly used in clinical treatment, such as berberine, tetrandrine, and stephanine, with the aim of providing a reference for drug researches and clinical applications. Isoquinoline AlkaloidsIsoquinoline alkaloids are widely distributed in 27 sections of plants, such as Menispermaceae, Berberidaceae, Papaveraceae, and Ranunculaceae [4]. Isoquinoline alkaloids, taking isoquinoline or tetrahydroisoquinoline as the basic parent nucleus, can be divided into 20 categories, including simple isoquinoline, benzylisoquinoline, phenethyl isoquinoline, naphthyl isoquinoline, aporphine, morphine,

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Tetrandrine ameliorates collagen-induced arthritis in mice by restoring the balance between Th17 and Treg cells via the aryl hydrocarbon receptor

Cited by 64 publications

References 43 publications

Autophagy induction enhances tetrandrine-induced apoptosis via the AMPK/mTOR pathway in human bladder cancer cells

Autophagy induction enhances tetrandrine-induced apoptosis via the AMPK/mTOR pathway in human bladder cancer cells

A critical review: traditional uses, phytochemistry, pharmacology and toxicology of Stephania tetrandra S. Moore (Fen Fang Ji)

Research Progress on Anti‐Inflammatory Effects and Mechanisms of Alkaloids from Chinese Medical Herbs

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