Tetraphenylethylene-based gemini surfactant as nonviral gene delivery system: DNA complexation, gene transfection and cellular tracking

Zhang, Kexin; Ding, Aixiang; Tan, Zheng-Li; Shi, You‐Di; Lu, Zhong‐Lin

doi:10.1016/j.jphotochem.2017.08.020

Cited by 13 publications

(17 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In consequence, the DNAs were subsequently transcripted into mRNA for translation. This proposed process matches well with our previous investigation using FL AIE vectors. ,, Overall, the intracellular fate of the DNA complex was clearly mapped, which would furnish an intuitive understanding of the transfection pathway.…”

Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

“…In this regard, fluorescent vectors with tracking capabilities stand out because they can provide visible information about some pivotal steps in the transfection process, which allows for the understanding of the transfection mechanism, leading to a better molecular design. 17 Particularly, molecules showing off− on fluorescence (FL) prevail over other common fluorescent molecules due to the on-demand indication. 18 For this purpose, the vector molecules are designed with an aggregation-induced emission (AIE) property, a type of fluorophore that only emits intensively in the aggregated state, 19−21 to switch on the self-indicating emission after compacting with the nucleic acids into nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Two-Photon Near-Infrared AIE Luminogens as Multifunctional Gene Carriers for Cancer Theranostics

Tang

Liu

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

Construction of multifunctional nonviral gene vectors to execute defined tasks holds great potential for the precise and effective treatment of gene-associated diseases. Herein, we have developed four large π-conjugation triphenylamine derivatives bearing two polar [12]aneN3 heads and a lipophilic tail for applications in gene delivery, one/two-photon-triggered near-infrared (NIR) fluorescence bioimaging, and combined photodynamic therapy (PDT) and gene therapy of cancer. These compounds possess typical NIR aggregation-induced emission characteristics, mega Stokes shifts, strong two-photon excitation fluorescence, and excellent DNA condensation abilities. Among them, vector 4 with a tail of n-hexadecane realized a transfection efficiency as high as 6.7 times that of the commercial transfection agent Lipofectamine 2000 in HEK293T cell lines. Using vector 4 as an example, transfection process tracking and ex vivo/in vivo tumoral imaging and retention with high resolution, high brightness, deep tissue penetration, and good biosafety were demonstrated. In addition, efficient singlet oxygen (1O2) generation by the DNA complex formed by vector 4 (4/DNA) resulted in effective PDT. Combined with anticancer gene therapy, collaborative cancer treatment with a dramatically enhanced cancer cell-killing effect was achieved. The development of this “three birds, one stone” approach suggests a new and promising strategy for better cancer treatment and real-time tracking of gene delivery.

show abstract

Section: Resultssupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Two-Photon Near-Infrared AIE Luminogens as Multifunctional Gene Carriers for Cancer Theranostics

Tang

Liu

et al. 2021

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

show abstract

“…Mostly, AIEgen-based bioprobes are directly used for PDT. ,,,,,,,,,− In some cases, the AIEgens are combined with traditional photosensitizers to fluorescently monitor the process of PDT. , In recent studies, the incorporation of AIEgens and peroxalates is also explored for the illumination-free production of ROS via a chemically initiated electron exchange luminescence process between high-energy intermediates and AIEgens . Third, AIEgen-based bioprobes can serve as a positively charged matrix to bind with negatively charged genetic materials (e.g., DNA or siRNA) via electrostatic adsorption for image-guided gene therapy. ,− Once delivered into mammalian cells, these genetic materials either silence the expression of diseased proteins or induce the expression of therapeutic proteins. Fourth, AIEgens with rotor-like structures typically show negligible fluorescence when existing as molecularly dissolved species.…”

Section: Bringing Aie Into Cancer Theranosticsmentioning

confidence: 99%

Aggregation-Induced Emission: A Trailblazing Journey to the Field of Biomedicine

Zhu

Kwok

Lam

et al. 2018

ACS Appl. Bio Mater.

267

190

View full text Add to dashboard Cite

The emergence of the aggregation-induced emission (AIE) concept significantly changes the cognition of the scientific community toward classic photophysical phenomena. More importantly, the AIE phenomenon has brought huge opportunities for the analysis of bioactive species, the monitoring of complicated biological processes, and the elucidation of key physiological and pathological behaviors. As a class of promising luminescent materials, AIE luminogens (AIEgens) are weakly or non-emissive in the form of isolated molecular species but emit particularly strong fluorescence in the aggregated and solid states. Motivated by the prominent advantages such as high brightness, large Stokes shift, excellent photostability, and good biocompatibility, AIEgen-based bioprobes have been widely explored in the field of biomedicine. This review aims to provide a systematic summary of the developmental history and an in-depth perspective of the current landscape of AIE in the biomedical field, with an emphasis on the discussions of major working principles. The milestones of the historical development of AIE in the biomedical field are first reviewed. A total of four major research directions are then extracted, including biomacromolecule sensing (at the molecular level), in vitro cell imaging (at the cellular level), in vivo imaging (at the animal level), and cancer theranostics (at the cellular and animal levels), together with clear-cut tables showing comprehensive cases for further study. Lastly, this review is concluded by the discussions of several perspectives on future directions. It is believed that AIEgen-based bioprobes will play vital roles in the exploration of mysterious life processes by integration with various cutting-edge modalities and techniques with an ultimate goal of addressing more healthcare issues.

show abstract

“…[4,5]. Gemini surfactants have a wide range of applications due to their unique properties, including enhanced oil recovery [6], transfection of genes [7], RNAdelivery [8] Inhibition of iron corrosion [9,10], and environmental protection [11][12][13]. Environmental friendly products to replace traditional surfactants can become a major trend as a result of rising governmental pressure and environmental protection.…”

Section: Introductionmentioning

confidence: 99%

Ester-Gemini and Monomeric Cationic Surfactants; Synthesis, Characterization, Surface Parameters and Biological Activity

et al. 2021

View full text Add to dashboard Cite

Gemini cationic surfactant is a promising generation of surfactants characterized by their unique surface properties and biological activities. Herein, a series of three Gemini cationic surfactants containing ester bond with different alkyl chain length and their corresponding monomeric structures have been synthesized. The monomeric surfactant were synthesized via simple esterification of different fatty acids (stearic, myrisitic, lauric acid) with 2-dimethyl amino ethanol, followed by quaternization with 1-bromohexane; while, and 1,6-dibromohexane was used to prepare the corresponding Gemini cationic surfactants. The structures of the synthesized compounds were elucidated using 1 H-NMR, FTIR spectroscopy. The biological activities and surface properties have been assayed and it was found that the prepared Gemini surfactants have higher antimicrobial efficiencies than the corresponding monomeric structure.

show abstract

Tetraphenylethylene-based gemini surfactant as nonviral gene delivery system: DNA complexation, gene transfection and cellular tracking

Cited by 13 publications

References 55 publications

Two-Photon Near-Infrared AIE Luminogens as Multifunctional Gene Carriers for Cancer Theranostics

Two-Photon Near-Infrared AIE Luminogens as Multifunctional Gene Carriers for Cancer Theranostics

Aggregation-Induced Emission: A Trailblazing Journey to the Field of Biomedicine

Ester-Gemini and Monomeric Cationic Surfactants; Synthesis, Characterization, Surface Parameters and Biological Activity

Contact Info

Product

Resources

About