Tetraselmis suecica biofilm cell destruction by high-pressure homogenization for protein extraction

Cell destruction level and metabolites green-extraction of Tetraselmis suecica by low and intermediate frequency ultrasound

“…As previously described, T. suecica cells are enclosed by a wall described in the literature as relatively resistant to attack by its surrounding, osmotic shock, temperature elevation or alkaline lysis [13] . The cell wall of T. suecica is of glycoprotein type [33] .…”

“…The biomass was characterized to determine its initial protein, carbohydrate and lipid composition as described by Delran et al [13] . Briefly, moisture content was determined by drying in an oven at 103 °C for 24 h. The ash fraction was measured by calcination at 550 °C for 12 h. Total protein was determined by the elemental analysis method using the conversion factor N = 6.25 [49] .…”

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

“…The biomass dilution, corresponding to a non-mechanical water extraction, was considered a blank for comparison with the other treatments in this study [20,29].…”

“…For instance, Zhang et al [19] found that 10 passes resulted in the highest extraction yield of proteins and carbohydrates from Parachlorella kessleri, showcasing the importance of exploring different pass numbers. Delran et al [20] studied the effect of the pressure applied and the number of passes on the destruction of Tetraselmis suecica cells and reported that a single pass at a pressure of 400 bars was sufficient for protein extraction. None of these studies investigated the effect of a pressure of 300 bars on Tetraselmis suecica cell disruption, and it is therefore possible that the threshold for disruption lies closer to 300 bars than to 400 bars.…”

Cell Destruction Level and Metabolites Green-Extraction of Tetraselmis Suecica by Low and Intermediate Frequency Ultrasound

“…The biomass was characterized to determine its initial protein, carbohydrate and lipid composition as described by Delran et al [13]. Briefly, moisture content was determined by drying in an oven at 103 • C for 24 h. The ash fraction was measured by calcination at 550 • C for 12 h. Total protein was determined by the elemental analysis method using the conversion factor N = 6.25 [49].…”