Tetrasomic Linkage Mapping of RFLP, PCR, and Isozyme Loci in<i>Lotus corniculatus</i>L

Fjellstrom, Robert G.; Steiner, J. J.; Beuselinck, P. R.

doi:10.2135/cropsci2003.1006

Cited by 7 publications

(2 citation statements)

References 51 publications

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“…DNA-based molecular markers such as amplified fragment length polymorphisms (AFLP) were used to confirm tetrasomic inheritance in the tetraploid forage legume species bird's-foot trefoil (Lotus corniculatus L.), which show suppressed quadrivalent pairing. AFLP successfully confirmed the tetrasomic inheritance prevailing in this species [58]. Similarly, tetrasomic inheritance was also evident in the other forage species such as alfalfa and bahiagrass (Paspalum notatum Fluggé) using DNA-based molecular markers systems [59][60][61].…”

Section: "Omics" Analysis Of Polyploidysupporting

confidence: 60%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

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Polyploidy means having more than two basic sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological (2n gametes) methods. This approach allows an increased gene scope and expression, thus resulting in phenotypic changes such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects that are partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages after optimizing methods, concentration and time, particularly when using colchicine. These experimental polyploids are a valuable tool for understanding gene expression, which seems to be driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers facilitated the identification of rare alleles for particular loci when compared with diploids, and also explained their heterozygosity, phenotypic plasticity and adaptability to diverse environments. Experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g., leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids should undergo selection for several generations to improve their performance and stability.

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Section: "Omics" Analysis Of Polyploidysupporting

confidence: 60%

Induced Polyploidy: A Tool for Forage Species Improvement

et al. 2021

View full text Add to dashboard Cite

show abstract

“…DNA-based molecular makers such as amplified fragment length polymorphism (AFLP) were applied to confirm tetrasomic inheritance in tetraploid forage legume species (Lotus corniculatus), which show suppressed quadrivalent pairing. AFLP successfully confirmed the tetrasomic inheritance prevailing in the species, providing evidence of polysomic tetraploid classification of this species[46]. Similarly, tetrasomic inheritance was also confirmed in the other forage species such as alfalfa, the Paspalum using DNA-based molecular markers systems[47,48,49].…”

mentioning

confidence: 60%

Scope of Whole Genome Duplication in Forages: A Plant Breeder Perspective

Rauf¹,

Ortíz²,

Malinowski³

et al. 2021

Preprint

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Polyploidy is a condition of having more than two sets of chromosomes. Polyploid plants may be artificially obtained through chemical, physical and biological methods (2n gametes). It is considered an interesting approach due to increased genescope and expression, thus resulting in phenotypic changes, such as yield and product quality. Nonetheless, breeding new cultivars through induced polyploidy should overcome deleterious effects partly contributed by genome and epigenome instability after polyploidization. Furthermore, shortening the time required from early chromosome set doubling to the final selection of high yielding superior polyploids is a must. Despite these hurdles, plant breeders have successfully obtained polyploid bred-germplasm in broad range of forages from optimized application methods, concentration and time mainly using colchicine. These experimental polyploids proved to be a valuable tool for understanding gene expression that is driven by dosage dependent gene expression, altered gene regulation and epigenetic changes. Isozymes and DNA-based markers aided on the identification of rare alleles for particular loci when compared with diploids, which may explain their heterozygosity, phenotypic plasticity and adaptability to diverse environments. It has also been observed that experimentally induced polyploid germplasm could enhance fresh herbage yield and quality, e.g. leaf protein content, leaf total soluble solids, water soluble carbohydrates and sucrose content. Offspring of experimentally obtained hybrids underwent selection for several generations to improve their performance and stability.

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